Nicotine Stimulates Osteoclast Resorption in a Porcine Marrow Cell Model

Henemyre, Claudia; Scales, Donald K.; Hokett, Steven D.; Cuenin, Michael F.; Peacock, Mark E.; Parker, M. Harry; Brewer, P.; Chuang, Augustine

doi:10.1902/jop.2003.74.10.1440

Cited by 71 publications

(66 citation statements)

References 38 publications

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“…Er et al [13] found that fibroblast metabolism could be altered by nicotine action and intercellular matrix synthesis was affected. It also affects periodontal ligament fibroblasts and stimulates osteoclasts activity [19,42]. In our study, nicotine administration caused a change in fibroblast activity due to the change of collagene fiber synthesis and decreased MMP2 expression.…”

Section: Discussionsupporting

confidence: 51%

Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane

et al. 2018

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Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of Matrix metalloproteinases-2 (MMP2) and Vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane. In this experimental study, the groups were divided into nicotine and control groups. While the nicotine groups in rats (n=6) were administered 2mg/kg Nicotine sulphate for 28 days, the control group in rats (n=6) were only administered 1,5 ml physiologic saline solution subcutaneously for 28 days. Histological sections were prepared and immunohistochemically stained for MMP2 and VEGF. The sections were stained with Trichrome-Masson were observed under light microscope. VEGF and MMP2 immunoreactivity of periodontal gingiva and dentin was assessed by immunohistochemical staining. In conclusion, nicotine effect reduces Matrix metalloproteinases production and disrupt collagen synthesis and causes periodontitis. We observed that nicotine increases periodontitis by disrupting periodontal membrane and prevent tooth to anchor in dental alveoli by disrupting epithelial structure.

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Section: Discussionsupporting

confidence: 51%

Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane

et al. 2018

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“…It also affects periodontal ligament fibroblasts (Pinto et al, 2002) and stimulates osteoclasts activity (Yuhara et al;Henemyre et al, 2003). The group treated with nicotine, cement and vimentin expression was observed in the periodontal membrane density irregularities along the collagen fibers were observed.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Nicotine on the Incisive Teeth and Expression of Vimentin in Rats

Deveci

Kalkanlı

et al. 2013

Int. J. Morphol.

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FARUK. E. R.; DEVECI, E.; KALKANLI, S. & DEVECI, B.The effects of nicotine on the incisive teeth and expression of vimentin in rats. Int. J. Morphol., 31(2):516-519, 2013. SUMMARY:Nicotine is an alkaloid toxic effects of oral cavity. In this study,14 adult Sprague-Dawley rats weighing 230-260 mg (±10 mg) were used as experimental animal. The rats of experimental group (n=6) were nicotinized systemically with nicotine sulphate, 2mg/kg subcutaneously, daily in period of 28 days. Pulp, alveolar bone tissue, periodontal membrane and gingival epithelial junction were investigated in these regions in incisive teeth longitudinal cross-section. Thinning of the collagen fibers in the pulp tissue, vascular congestion, and inflammatory cell infiltration were observed. Mesenchymal tissues that is stained positive for vimentin lay underneath the epithelium. A strong expression of vimentin can be observed in formed periodontal ligament.

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“…Previous in vitro studies reported that nicotine stimulated the formation and differentiation of osteoclasts, 7,8 suppressed osteogenesis in osteoblasts, directly stimulated osteoclast precursors, and induced an imbalance of osteoblasts and osteoclasts in vitro. 9, 10 Rothem et al 11 suggested that nicotine affected osteoblast cell proliferation in a biphasic manner, including toxic and antiproliferative effects at high levels of nicotine and stimulatory effects at low levels.…”

Section: Introductionmentioning

confidence: 95%

Short-term effects of nicotine on orthodontically induced root resorption in rats

Wang

et al. 2015

The Angle Orthodontist

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Objective: To investigate the effect of nicotine exposure on root resorption in an in vivo rat model of orthodontic tooth movement (OTM), and its association with odontoclastogenesis and receptor activator of nuclear factor-kappa B ligand (RANKL) expression. Materials and Methods: Forty-eight 10-week-old male Wistar rats were divided into three groups. The negative control group was untreated. The left maxillary first molars in the nicotine-treated group and the positive control group received OTM with an initial force of 0.6 N in the mesial direction. Also, the nicotine-treated group received intraperitoneal injection of nicotine at 7 mg/kg per day. After 21 days, the rats were humanely killed. Eight rats from each group were randomly chosen for crater volume analysis by micro-computed tomography. For the remaining eight rats in each group, specimen slices were generated for histologic examination to determine the odontoclast number and the mean optical density value of RANKL. Results: The resorption volumes in the nicotine-treated group were significantly larger than those in the control groups. Also, the nicotine-treated group displayed significantly higher number of odontoclasts and elevated RANKL expression compared to the control groups. Conclusions: In an in vivo rat model, nicotine exposure promotes odontoclastogenesis and RANKL expression, evoking aggravated root resorption during OTM.

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Nicotine Stimulates Osteoclast Resorption in a Porcine Marrow Cell Model

Cited by 71 publications

References 38 publications

Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane

Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane

The Effects of Nicotine on the Incisive Teeth and Expression of Vimentin in Rats

Short-term effects of nicotine on orthodontically induced root resorption in rats

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