Nilai Diagnostik Antigen Tb Dengan Rapid Test Device (Tb Ag) Untuk Tuberkulosis Paru

Abstract

In Indonesia, the diagnosis of pulmonary tuberculosis relies primarily on an identification of acid-fast bacilli on sputum smears. However, microscopic device has several limitations. The sensitivity of microscopic examination is variable. The quality of smear microscopic results is heavily depend on the workload, and the skill of the technician’s reading the slide. TB antigen rapid test device (TB Ag) is fast, easy and does not either need skillness of the operator. The kit detects specific secreted antigen M.tuberculosis coded by: RD (Region of Difference) 1, RD2 and RD3. These RD1−3 were found deleted from BCG (Bacille Calmette-Guerine) vaccine strain. In the present study, the diagnostic value of TB Ag was assessed. Sputum samples were examined from 59 suspected tuberculosis patients and 22 non tuberculosis patients. The samples of the suspected tuberculosis patients were collected as three consecutive sputum specimens (spot, morning, spot). The total 199 specimens were examined by sputum smear microscopy and TB Ag. M.tuberculosis culture by using Lowenstein Jensen media, which was used as a gold standard. The sensitivity and specificity of microscopic sputum smear were 83.8% (95% CI: 70.0–89.4) and 96.3% (95% CI: 89.8–98.7), respectively. While, the sensitivity and specificity of TB Ag were 72.6% (95% CI: 63.9–79.9) and 90.9% (95% CI: 72.2–97.5), respectively. The concordance between microscopic sputum smear and TB Ag was 70.8%. TB Ag can be considered as a new diagnostic tool for the diagnosis of pulmonary tuberculosis, especially at the health services where there is no expert technician available for microscopic sputum smear examination.

The Examination of ESAT-6, CFP-10, MPT-64 Antigens of Mycobacterium tuberculosis in Urine of Pediatric Tuberculosis Patient with Immunochromatography to Support the Diagnosis

“…The immunochromatographic method is a rapid test and has high validity (Gustiani et al 2014). In another study, it was concluded that TBAg rapid test can be used as new diagnostic method in pulmonary TB, especially in inadequate health services (Sari 2011). There are several studies on TBAg rapid test, but the use of urine samples in pediatric TB patient has never been performed before.…”

Validity of Examination of Complex Specific Antigen Mycobacterium Tuberculosis Rapid Immunochromatography Method in Patients with Pulmonary Tuberculosis

“…The sensitivity and specificity of the results of the current study are different from studies conducted by other studies, Sari research (2011) stated that the sensitivity value of the TB Ag rapid test conducted in Surabaya was 72.6% and a specificity of 90.9%. That research concluded that the TB Ag rapid test could be considered as a new diagnostic tool for the diagnosis of pulmonary tuberculosis, especially in healthcare settings that do not have laboratory technicians who are experienced in examining the staining results of acid-resistant bacilli (Sari & Aryati, 2016). According to research conducted by Shen et al (2011), it was stated that to identify ESAT6/CFP10 by rapid ICT, a minimum concentration of M. tuberculosis in culture isolates is required of 3x104 to 3x105 CFU/ml.…”

Kesesuaian Uji Cepat Antigen Mycobacterium tuberculosis dengan Pulasan BTA pada Tersangka Tuberkulosis Paru

“…10 Penelitian yang dilakukan Sari tahun 2010 mendapatkan sensitivitas dan spesifisitas uji cepat antigen M. tuberculosis masing-masing 72,6% dan 90,9%. 11 Deteksi antigen TB telah dikembangkan melalui temuan antigen spesifik yang berasal dari protein yang disekresi oleh M. tuberculosis. Antigen protein tersebut adalah early secretory antigenic target 6 (ESAT6), culture filtrate protein 10 (CFP10), dan Mycobacterium protein tuberculosis 64 (MPT64), yang disandi oleh gen region of diffences 1 (RD1), RD2, dan RD3.…”

“…10 Tidak jauh berbeda dengan yang didapatkan pada penelitian di Bandung yaitu sensitivitas dan spesifisitas uji cepat antigen M. tuberculosis masing-masing 72,6% dan 90,9% sedang sensitivitas dan spesifisitas pulasan BTA 83,8% dan 96,3%. 11 Penelitian tentang kesesuaian yang sama dengan peneliti belum ada temukan.…”