Specific food protein and peptides could prevent hypertension pass through mechanism of inhibition of enzyme activity of angiotensin converting enzyme (ACE) or ACE inhibition. Fermented buffalo milk or dadih has the potential to be developed into antihypertensive food. The purpose of this study was to investigate optimum fermentation time based on characteristics, measure the acidity of dadih, protein extraction, degree of hydrolysis, dissolved protein level and the activity of ACE inhibition. Protein extract was fractionated using ultrafiltration membrane assisted with centrifuge and then do the activity of ACE inhibition as ACE inhibitor. Buffalo milk was fermented for 0, 1, 2, 3 and 4 days, zero day as a control. Protein of dadih was extracted using alcohol solution then was fractionated. The fraction that has the highest the activity was analyzed their amino acid composition using ultra performance liquid chromatograph. The result are the best fermentation time during one day, dissolved protein levels was 912.73 ppm, and the highest ACE inhibitor activity was 87.52% and the degree of hydrolysis was 23.38%. Fractionation results based on molecular weight was below 3 kDa, this molecule have the highest ACE inhibitor activity was 99.3%.The amino acid composition that we got was threonine (0.0249%,) proline (0.039%), alanine (0.045%), phenyl alanine smaller than 0.012% and glutamic acid 0.158% respectively. We conclude that dadih can inhibit ACE activity and it has the potential to be developed into functional food.