Nine-Analyte Detection Using an Array-Based Biosensor

Taitt, Chris R.; Anderson, George P.; Lingerfelt, Brian M.; Feldstein, s. Mark. J.; Ligler, Frances S.

doi:10.1021/ac0260185

Cited by 135 publications

(65 citation statements)

References 47 publications

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“…The biosensor takes advantage of the planar waveguide coupled with PDMS flow cells to allow simultaneous detection and quantification of multiple target analytes (up to nine currently demonstrated) in multiple samples. 33 Due to public health risks, much of the recent work in assay development has concentrated on the rapid detection of foodborne contaminants. The NRL Array Biosensor has been used for the measurement of a number of protein toxins, such as staphylococcal enterotoxin B (SEB) 22,23 and botulinum toxoid A, 22 and bacteria, including Campylobacter spp., 29 Shigella spp., 29 Escherichia coli 32 and Salmonella enterica serovar Typhimurium, 28 in a variety of spiked food matrices.…”

Section: Antibody-based Detectionmentioning

confidence: 99%

The Array Biosensor: Portable, Automated Systems

et al. 2007

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Section: Antibody-based Detectionmentioning

confidence: 99%

The Array Biosensor: Portable, Automated Systems

et al. 2007

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“…In addition, classic CT determinations require the use of either animal methods (5,6) or tissue culture methods (7,8), which are also time-consuming and are subjective in the interpretation of results. Efforts have been made recently to develop more-sensitive methods, including enzyme-linked immunosorbent assays (9), latex agglutination assays (10), coagglutination assays (5), liposome-based assays (9,11), radioimmunoassays (12), hydrogel-based immunoassays (13), monosaccharide-and antibody array-based assays (14)(15)(16)(17), PCR-based molecular assays (10,(18)(19)(20)(21), and biosensor-based assays (22)(23)(24)(25)(26)(27)(28)(29)(30)(31). A reliable laboratory tool is desirable for clinical services and epidemiological investigation, to characterize CT activities rapidly and quantitatively.…”

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confidence: 99%

Quantitative Detection of Vibrio cholera Toxin by Real-Time and Dynamic Cytotoxicity Monitoring

et al. 2013

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eWe report here the quantitative detection of Vibrio cholerae toxin (CT) in isolates and stool specimens by dynamic monitoring of the full course of CT-mediated cytotoxicity in a real-time cell analysis (RTCA) system. Four cell lines, including Y-1 mouse adrenal tumor cells, Chinese hamster ovary (CHO) cells, small intestine epithelial (FHs74Int) cells, and mouse adrenal gland (PC12-Adh) cells, were evaluated for their suitability for CT-induced cytotoxicity testing. Among them, the Y-1 line was demonstrated to be the most sensitive for CT-mediated cytotoxicity, with limits of detection of 7.0 pg/ml for purified CT and 0.11 ng/ml for spiked CT in pooled negative stool specimens. No CT-mediated cytotoxicity was observed for nontoxigenic V. cholerae, non-V. cholerae species, or non-V. cholerae enterotoxins. The CT-RTCA assay was further validated with 100 stool specimens consecutively collected from patients with diarrhea and 200 V. cholerae isolates recovered from patients and the environment, in comparison to a reference using three detection methods. The CT-RTCA assay had sensitivities and specificities of 97.5% and 100.0%, respectively, for V. cholerae isolates and 90.0% and 97.2% for stool specimens. For stool specimens spiked with CT concentrations ranging from 3.5 pg/ml to 1.8 ng/ml, the inoculation-to-detection time was 1.12 ؎ 0.38 h, and the values were inversely correlated with CT concentrations ( ‫؍‬ ؊1; P ‫؍‬ 0.01). The results indicate that the CT-RTCA assay with the Y-1 cell line provides a rapid and sensitive tool for the quantitative detection of CT activities in clinical specimens. Vibrio cholerae is a Gram-negative, comma-shaped, bacterial pathogen causing cholera, an acute secretory diarrheal disease. Epidemic cholera is common in developing countries and affects about 100,000 people annually (1). Cholera toxin (CT) is a major virulence determinant of V. cholerae, leading to rapidly progressing dehydration, shock, metabolic acidosis, and even death within hours without adequate and appropriate therapy (2). CT is a key biomarker of V. cholerae that is used for forecasting and assessing epidemic disease, monitoring and controlling cholera outbreaks, preventing epidemics, and guiding medical personnel in providing timely treatment of patients. There has been urgent demand for the development of novel sensitive assays for the detection and identification of CT proteins.Conventional biochemical and immunological methods for CT detection and identification can be completed only after V. cholerae is isolated. The bacterial isolation and subsequent CT detection and identification are laborious and usually require several days, resulting in significant delays in patient care and disease control (3, 4). In addition, classic CT determinations require the use of either animal methods (5, 6) or tissue culture methods (7,8), which are also time-consuming and are subjective in the interpretation of results. Efforts have been made recently to develop more-sensitive methods, including enzyme-linked immunosorbent...

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“…In the array biosensor, target analytes are captured by antibodies on a patterned glass slide. Fluorescent reporter antibodies are added and, after evanescent wave excitation by a laser, images are recorded by a charge-coupled device camera (68,179,276,277). The array biosensor has been tested with target analytes in different complex matrices, including toxins in clinical fluids, environmental samples and food matrices (179), and bacteria in food (251,277).…”

Section: Immunological Detectionmentioning

confidence: 99%

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

et al. 2005

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SUMMARY Recent events have made public health officials acutely aware of the importance of rapidly and accurately detecting acts of bioterrorism. Because bioterrorism is difficult to predict or prevent, reliable platforms to rapidly detect and identify biothreat agents are important to minimize the spread of these agents and to protect the public health. These platforms must not only be sensitive and specific, but must also be able to accurately detect a variety of pathogens, including modified or previously uncharacterized agents, directly from complex sample matrices. Various commercial tests utilizing biochemical, immunological, nucleic acid, and bioluminescence procedures are currently available to identify biological threat agents. Newer tests have also been developed to identify such agents using aptamers, biochips, evanescent wave biosensors, cantilevers, living cells, and other innovative technologies. This review describes these current and developing technologies and considers challenges to rapid, accurate detection of biothreat agents. Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents.

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Nine-Analyte Detection Using an Array-Based Biosensor

Cited by 135 publications

References 47 publications

The Array Biosensor: Portable, Automated Systems

The Array Biosensor: Portable, Automated Systems

Quantitative Detection of Vibrio cholera Toxin by Real-Time and Dynamic Cytotoxicity Monitoring

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

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