Nine Genes Mediate the Therapeutic Effects of Iodine-131 Radiotherapy in Thyroid Carcinoma Patients

Abstract

Background. Thyroid carcinoma (THCA) is one of the most common malignancies of the endocrine system, which is usually treated by surgery combined with iodine-131 (I131) radiotherapy. Aims. This study is aimed at exploring the potential targets of I131 radiotherapy in THCA. Methods. The RNA-sequencing data of THCA in The Cancer Genome Atlas database (including 568 THCA samples) was downloaded. The differentially expressed genes (DEGs) between the tumour samples whether or not subjected to I131 radiotherapy were identified using edgeR package. Using the WGCNA package, the module that was relevant with I131 radiotherapy was selected. The intersection genes of the hub module nodes and the DEGs were obtained as hub genes, followed by the function and pathway enrichment analyses using the clusterProfiler package. Moreover, the protein-protein interaction (PPI) network for the hub genes was constructed using Cytoscape software. In addition, more important hub genes were analysed with function mining using the GenCLiP2 online tool. The qPCR analysis was used to verify the mRNA expression of more important hub genes in THCA tissues. Results. There were 500 DEGs (167 upregulated and 333 downregulated) between the two groups. WGCNA analysis showed that the green module (428 nodes) exhibited the most significant correlation with I131 radiotherapy. A PPI network was built after the identification of 53 hub genes. In the PPI network, CDH5, KDR, CD34, FLT4, EMCN, FLT1, ROBO4, PTPRB, and CD93 exhibited higher degrees, which were mainly implicated in the vascular function. The relative expression of nine mRNAs in the THCA tissues treated with I131 was lower. Conclusion. I131 radiotherapy might exert therapeutic effects by targeting CDH5, KDR, CD34, FLT4, EMCN, FLT1, ROBO4, PTPRB, and CD93 in THCA patients.

A Potential Nine-lncRNAs Signature Identification and Nomogram Diagnostic Model Establishment for Papillary Thyroid Cancer

“…For example, a total of 11 genes have been reported to exert important roles in PTC relapse, which shed novel insights in the lymph node metastasis of PTC [ 39 ]. Han et al determined that nine genes containing CDH5, KDR, CD34, FLT4, EMCN, FLT1, ROBO4, PTPRB, and CD93 could mediate the I 131 radiotherapy in TC patients [ 40 ]. Three genes (NGF, FOS, and GRIA1) are found to be involved in regulation of the features of TC stem cells using WGCNA [ 41 ].…”

Integrative Analysis of Multi-Omics Identified the Prognostic Biomarkers in Acute Myelogenous Leukemia

“…In this study, we clustered AML samples into three consensus clusters at k = 3, and the DMGs were identified and validated in AML, including CD34, HOXA7, and CD96. CD34 plays as a role in response to iodine-131 radiotherapy in thyroid carcinoma ( 29 ). HOXA7 is a member of the HOX family which was involved in the development of multiple cancer types ( 30 ).…”

The Impact of Iodine Concentration Disorders on Health and Cancer

“…Scintigraphy is also important in assessing the prognosis in patients with heart failure, where the catecholamine analog, 123I-meta-iodobenzylguanidine (MIBG), is employed [ 10 ]. Radioiodine therapy is an effective treatment in the case of hyperthyroidism in patients with Graves’ disease, toxic adenoma, and toxic multinodular goiter, and in patients with well-differentiated thyroid cancer [ 11 ]. The radioactive iodine is taken up and accumulated by the hyperactive thyroid gland or by differentiated thyroid tumors.…”