Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity

Bai, Meng; Cheng, Yahui; Sun, Xiaowei; Wang, Ziyi; Wang, Yongxia; Cui, Xiao‐Long; Xiao, Wei

doi:10.3390/v11070615

Cited by 20 publications

(12 citation statements)

References 48 publications

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“…3). Bai et al (2019) (57) reported that from 51 complete genome sequences of Aeromonas phages in GenBank, the majority of Aeromonas phages were double-strand DNA phages belonging to the Myoviridae (33/51), Podoviridae (7/51) and Siphoviridae (5/51) families, along with one ssDNA virus and five unclassified bacteriophages (58–63).…”

Section: Resultsmentioning

confidence: 99%

WITHDRAWN: Isolation and Characterization of the Lytic Bacteriophages and Its Application in Combination with Amoxicillin against Aeromonas dhakensis

Sawaengwong

Sunthornthummas

Wongyoo

et al. 2022

Preprint

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Aeromonas dhakensis is the most virulent Aeromonas species in tropical and subtropical areas and causes a variety of human diseases. Owing to its resistance against some antibiotics, there is an urgent need for new strategies against this pathogen. This is the first study to isolate and characterize lytic phages against A. dhakensis. Of Aeromonas isolates, only isolate AM could be used to isolate phages using the enrichment technique. In silico detection of antimicrobial resistance genes and virulence factors corresponding to the main bacterial virulence determinants were observed in A. dhakensis AM, which was then used as the host for phage isolation. Four lytic phages, designated vB_AdhS_TS3, vB_AdhM_TS9, vB_AdhM_DL, and vB_AdhS_M4, were isolated. Transmission electron micrographs showed that vB_AdhS_TS3 and vB_AdhS_M4 belonged to Siphoviridae family, whereas vB_AdhM_TS9 and vB_AdhM_DL belonged to Myoviridae family. The pre-treatment (co-inoculation of A. dhakensis and phage) with individual phages and phage cocktails reduced bacterial numbers in the range of 2.82-6.67 and 5.19-5.43 log CFU/mL, respectively, after 6 h of incubation. In post-treatment, the maximum inactivation was achieved with the log reduction of bacterial number in the range of 3.06-5.25 and 4.01-6.49 log CFU/mL after 6 and 12 h of incubation, respectively. A combination of phage cocktail with amoxicillin at sub-MIC showed complete inactivation in pre-treatment and post-treatment The complete genome and G+C content of phages vB_AdhS_TS3, vB_AdhM_DL, and vB_AdhM_TS9 were 115,560, 61,429, and 115,503 bp, respectively. This study demonstrated using phages as an adjuvant with a sublethal concentration of antibiotics as an effective therapeutic strategy.

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Section: Resultsmentioning

confidence: 99%

WITHDRAWN: Isolation and Characterization of the Lytic Bacteriophages and Its Application in Combination with Amoxicillin against Aeromonas dhakensis

Sawaengwong

Sunthornthummas

Wongyoo

et al. 2022

Preprint

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“…Transmission electron microscopy suggested that Akh-2 belongs to the Siphoviridae family due to its icosahedral head and long non-contractile tail. Among the 60 viruses that infect the Aeromonas species, including nine novel phages reported recently [13], only six belong to the Siphoviridae family, and only 3 (AhSzq-1, AhSzw-1, and 4L372X) infect A. hydrophila [13,20]. Although possible applications of these viruses in fish disease control have been suggested, they have not been tested.…”

Section: Discussionmentioning

confidence: 99%

“…The genome was submitted to NCBI under accession number MK318083.1. Among the 17 A. hydrophila and Aeromonas phages of the Siphoviridae family listed by Bai et al [13], phage Akh-2 showed the highest homology to AhSzw-1, with an overall nucleotide sequence identity of 74% ( Figure 5). (10 7PFU/mL) was maintained at the indicated pH levels for three days, and then the titer was determined by plaque assay.…”

Section: Genomic Analysismentioning

confidence: 97%

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

et al. 2020

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Bacteriophages are increasingly being used as biological control agents against pathogenic bacteria. In the present study, we isolate and characterize bacteriophage Akh-2 from Geoje Island, South Korea, to evaluate its utility in controlling motile Aeromonas septicemia. Akh-2 lysed four of the seven Aeromonas hydrophila strains tested. Transmission electron microscopy analysis showed that Akh-2 belongs to the Siphoviridae family, with head and tail sizes of 50 ± 5 and 170 ± 5 nm, respectively. One-step growth curve analysis revealed that the phage has a latent period of 50 ± 5 min and a burst size of 139 ± 5 plaque-forming units per infected cell. The phage appeared stable in a pH range of 6–8 and a temperature range of −80 to 46 °C. Based on next-generation sequencing analysis, its genome is 114,901 bp in size, with a 44.22% G + C content and 254 open reading frames. During an artificial induction of the disease, loach (Misgurnus anguillicaudatus) treated with Akh-2 showed an increased survival rate and time compared with the non-treated control. Our results suggest that Akh-2 is a potential biological agent for the treatment of Aeromonas infections in fish.

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“…These bacteria are usually resistant to first and second line antibiotic therapy involving beta-lactam drugs and third generation cephalosporins via mechanisms such as resistance genes and biofilm formation (Janda and Abbott, 2010). Bacteriophages, which have been suggested as an alternative to antibiotics, have been isolated against environmental and fish pathogen strains of A. hydrophila (Chow and Rouf, 1983;Merino et al, 1990a,b;Gibb and Edgell, 2007;Shen et al, 2012;Jun et al, 2013;Anand et al, 2016;Wang et al, 2016;Le et al, 2018;Yuan et al, 2018;Bai et al, 2019;Cao et al, 2019;Kazimierczak et al, 2019). The host range of these bacteriophages, however, was not reported to extend to clinical strains.…”

Section: Discussionmentioning

confidence: 99%

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

et al. 2020

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The increase in global warming has favored growth of a range of opportunistic environmental bacteria and allowed some of these to become more pathogenic to humans. Aeromonas hydrophila is one such organism. Surviving in moist conditions in temperate climates, these bacteria have been associated with a range of diseases in humans, and in systemic infections can cause mortality in up to 46% of cases. Their capacity to form biofilms, carry antibiotic resistance mechanisms, and survive disinfection, has meant that they are not easily treated with traditional methods. Bacteriophage offer a possible alternative approach for controlling their growth. This study is the first to report the isolation and characterization of bacteriophages lytic against clinical strains of A. hydrophila which carry intrinsic antibiotic resistance genes. Functionally, these novel bacteriophages were shown to be capable of disrupting biofilms caused by clinical isolates of A. hydrophila. The potential exists for these to be tested in clinical and environmental settings.

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Nine Novel Phages from a Plateau Lake in Southwest China: Insights into Aeromonas Phage Diversity

Cited by 20 publications

References 48 publications

WITHDRAWN: Isolation and Characterization of the Lytic Bacteriophages and Its Application in Combination with Amoxicillin against Aeromonas dhakensis

WITHDRAWN: Isolation and Characterization of the Lytic Bacteriophages and Its Application in Combination with Amoxicillin against Aeromonas dhakensis

Isolation, Characterization, and Application of a Bacteriophage Infecting the Fish Pathogen Aeromonas hydrophila

Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains of Aeromonas hydrophila

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