Various controlled atmospheres and chemical treatments on postharvest nitrate-nitrite conversion in fresh spinach (Spinaciu oleracea L.) were investigated. Substantial 'nitrite accumulated in spinach held in low 0, (1%) at 10°C for lo-15 days relative to that held in air. High CO, (15-18%) at 10°C reduced nitrite accumulation provided the storage period did not exceed 10 days. Nitrate reductase activity was reduced 80% in leaves held for 2 days at 10°C and could not be detected in leaves held for 5 days or longer. Leaves dipped in 1% and 5% DHA-S and subsequently held for 15 days at 10°C showed signs of physiological injury and accumulated high levels of nitrite, particularly at the 1% concentration, relative to water-dipped controls where some nitrite accumulated but no leaf injury was seen. Compared to a water dip, nitrite accumulation was somewhat lessened by dipping in a 500 ppm chloramphenicol solution. Little conversion of nitrate to nitrite occurred in fresh spinach held dry. Nitrite-N levels exceeding 20 ppm were found only in visibly decayed samples.