Nitrate–NRT1.1B–SPX4 cascade integrates nitrogen and phosphorus signalling networks in plants

Hu, Bin; Jiang, Zhimin; Wang, Wei; Qiu, Yahong; Zhang, Zhihua; Yong-qiang, Liu; Li, Aifu; Gao, Xiaokai; Liu, Linchuan; Qian, Yangwen; Huang, Xiahe; Yu, Feifei; Kang, Shenghong; Wang, Yiqin; Xie, Junpeng; Cao, Shuting; Zhang, Lianhe; Wang, Yingchun; Xie, Qi; Kopriva, Stanislav; Chu, Chengcai

doi:10.1038/s41477-019-0384-1

Cited by 323 publications

(310 citation statements)

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“…Thus, these findings uncovered a mechanism not only for nitrate signal propagation from the plasma membrane to the nucleus, but also for integration of nitrate and phosphate signaling (Hu et al, 2019). This regulatory event is vital for signal transmission, but the essential component to understand the working mechanism of OsSPX4 degradation is lacking.…”

Section: Reviewmentioning

confidence: 99%

“…As mentioned above, AtNIGT1s are under the control of both nitrate and phosphate starvation signals, and expression of the phosphate starvation-induced transcript is also dependent on nitrate provision, indicating that the upstream nitrate signal sensing and transduction underlies N-P interaction. Indeed, a recent study revealed that nitrate plays an essential role in activating phosphate utilization (Hu et al, 2019). Nitrate activates the expression of PSI genes, and this regulatory event is dependent on the nitrate sensor OsNRT1.1B, which further indicates the direct interaction between signaling pathways of nitrate and phosphate (Hu et al, 2015(Hu et al, , 2019.…”

Section: Reviewmentioning

confidence: 99%

“…Indeed, a recent study revealed that nitrate plays an essential role in activating phosphate utilization (Hu et al, 2019). Indeed, a recent study revealed that nitrate plays an essential role in activating phosphate utilization (Hu et al, 2019).…”

Section: Reviewmentioning

confidence: 99%

“…Indeed, a recent study revealed that nitrate plays an essential role in activating phosphate utilization (Hu et al, 2019). Interestingly, OsNRT1.1B can interact with OsSPX4 and promotes its degradation, and their interaction is highly facilitated by nitrate (Hu et al, 2019). OsNRT1.1B represents the frontier for perceiving nitrate signals on the plasma membrane, while OsPHR2 represents the frontier for activating PSI gene expression in the nucleus.…”

Section: Reviewmentioning

confidence: 99%

“…Based on current knowledge, the signaling network for the NRT1.1-SPX cascade and NIGT1s involved in N-P balance can be understood (Fig. It has been observed that nitrate-induced expression of PSI genes is greatly enhanced under phosphate starvation (Hu et al, 2019;Medici et al, 2019). Under low nitrate conditions, regardless of phosphate availability, the SPX protein accumulates to a relatively high level, thus retaining both PHR and NLP in the cytoplasm and repressing the expression of both PSI genes and nitrate-responsive genes (Fig.…”

Section: Regulation Of the N-p Interaction Is Modulated At Differementioning

confidence: 99%

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Nitrogen–phosphorus interplay: old story with molecular tale

Chu

2019

New Phytologist

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Summary Nitrogen (N) and phosphorus (P) are the two most abundant mineral nutrients used by plants, and are also the mostly widely used fertilizer elements driving crop yield improvement in agricultural production. The coordinated utilization of N and P is essential to maintain optimal plant growth and achieve maximal crop yield. The signaling pathways of N and P are generally studied separately, so our understanding of N–P interactions is very limited. A series of recent studies have revealed the critical components regulating N–P interactions in both Arabidopsis thaliana and rice (Oryza sativa), and have shed light on our in‐depth understanding of the network integrating N and P signaling pathways. Here, we summarize recent progress on N–P interaction and propose possible working mechanisms integrating these N–P interactive regulation pathways. We further discuss future work that might reveal the N–P interactive regulation network in plants.

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Section: Regulation Of the N-p Interaction Is Modulated At Differementioning

confidence: 99%

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Nitrogen–phosphorus interplay: old story with molecular tale

Chu

2019

New Phytologist

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Thepho1;2a′‐m1.1allele ofPhosphate1conditions misregulation of the phosphorus starvation response in maize (Zea maysssp.maysL.)

et al. 2022

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Plant PHO1 proteins play a central role in the translocation and sensing of inorganic phosphate. The maize (Zea mays ssp. mays) genome encodes two co-orthologs of the Arabidopsis PHO1 gene, designated ZmPho1;2a and ZmPho1;2b. Here, we report the characterization of the transposon footprint allele Zmpho1;2a 0 -m1.1, which we refer to hereafter as pho1;2a. The pho1;2a allele is a stable derivative formed by excision of an Activator transposable element from the ZmPho1;2a gene. The pho1;2a allele contains an 8-bp insertion at the point of transposon excision that disrupts the reading frame and is predicted to generate a premature translational stop. We show that the pho1;2a allele is linked to a dosage-dependent reduction in Pho1;2a transcript accumulation and a mild reduction in seedling growth. Characterization of shoot and root transcriptomes under full nutrient, low nitrogen, low phosphorus, and combined low nitrogen and low phosphorus conditions identified 1100 differentially expressed genes between wild-type plants and plants carrying the pho1;2a mutation. Of these 1100 genes, 966 were upregulated in plants carrying pho1;2a, indicating the wildtype PHO1;2a to predominantly impact negative gene regulation. Gene set enrichment analysis of the pho1;2a-misregulated genes revealed associations with phytohormone signaling and the phosphate starvation response. In roots, differential expression was broadly consistent across all nutrient conditions. In leaves, differential expression was largely specific to low phosphorus and combined low nitrogen and low phosphorus conditions. Of 276 genes upregulated in the leaves of pho1;2a mutants in the low phosphorus condition, 153 were themselves induced in wild-type plants with respect to the full nutrient condition. Our observations suggest that Pho1;2a functions in the fine-tuning of the transcriptional response to phosphate starvation through maintenance and/or sensing of plant phosphate status.

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