Nitrate reductase and glutamine synthetase activity, internal N pools, and growth of Ulva lactuca: responses to long and short-term N supply

Teichberg, Mirta; Heffner, Leanna; Fox, Sophia E.; Valiela, Iván

doi:10.1007/s00227-006-0561-4

Cited by 56 publications

(34 citation statements)

References 44 publications

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“…The δ 15 N of fertilizers used in our enrichments were -2.8 ‰ and -0.6 ‰ for NO 3 -and NH 4 + , respectively, values that are relatively light compared to the source of ambient DIN in the 2 estuaries. The decrease in the δ 15 N signatures in fronds enriched with NO 3 -and NH 4 + is a reflection of the change in internal N pools within fronds as ambient DIN was replaced by fertilizer N. In an earlier study, we found that this change in U. lactuca took place over 2 to 5 d (Teichberg et al 2007). The lighter δ 15 N signatures indicate rapid assimilation of the fertilizer source by the end of the incubation period.…”

Section: Internal Nutrient Poolsmentioning

confidence: 71%

“…Ancillary experiments with open cages during the time of the experiments showed a similar loss in % tissue N (data not shown). Therefore, we believe the first 2 factors were responsible for the loss in % tissue N, which we have discussed more thoroughly in another study (Teichberg et al 2007). The C:N of the macroalgal fronds differed among species, sites, and nutrient treatments (Fig.…”

Section: Internal Nutrient Poolsmentioning

confidence: 83%

“…have higher affinity for NH 4 + , Naldi & Wheeler 2002. The ability to take up NO 3 -may depend on the level of nitrate reductase activity in the algae (Lartigue & Sherman 2005, Teichberg et al 2007. In another study, we found nitrate reductase activity to be significantly lower in G. tikvahiae than in U. lactuca (M. Teichberg unpubl.…”

Section: Introductionmentioning

confidence: 85%

See 2 more Smart Citations

Macroalgal responses to experimental nutrient enrichment in shallow coastal waters: growth, internal nutrient pools, and isotopic signatures

Teichberg¹,

Fox²,

Aguila³

et al. 2008

Mar. Ecol. Prog. Ser.

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Increased nutrient inputs to temperate coastal waters have led to increased occurrences of macroalgal blooms worldwide. To identify nutrients that are limiting to macroalgae and to determine whether different forms of these nutrients and long-term ambient nutrient conditions affect macroalgal response, we used in situ enrichment methods and tested the response of 2 bloom-forming species of macroalgae, Ulva lactuca and Gracilaria tikvahiae, from shallow estuaries of Waquoit Bay, Massachusetts, USA, that receive different land-derived N inputs. We enriched caged macroalgal fronds with nitrate, ammonium, phosphate, and N + P combinations, and measured growth, nutrient content, and δ 15 N signatures of fronds after 2 wk of incubation. In these estuaries, P did not limit growth, however, the 2 species differed in growth response to N additions. Growth of U. lactuca was greater in Childs River (CR), the estuary with higher nitrate inputs, than in Sage Lot Pond (SLP); growth in SLP increased with nitrate and ammonium enrichment. In contrast, growth of G. tikvahiae was greater in SLP than in CR, but had no growth response to N enrichment in either site. C and N contents differed initially between species and sites, and after nutrient enrichment. Final tissue % N increased and C:N decreased after nitrate and ammonium enrichment. δ 15 N values of the macroalgae demonstrated uptake of the experimental fertilizers, and a higher affinity and faster turnover of internal N pools with ammonium than nitrate enrichment in both species. We suggest that U. lactuca blooms in areas with both high nitrate and ammonium water column concentrations, and is more N-limited in oligotrophic waters where DIN levels are too low to sustain high growth rates. G. tikvahiae has a greater N storage capacity than U. lactuca, which may allow it to grow in less nutrientrich waters.

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Section: Internal Nutrient Poolsmentioning

confidence: 71%

Section: Internal Nutrient Poolsmentioning

confidence: 83%

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Macroalgal responses to experimental nutrient enrichment in shallow coastal waters: growth, internal nutrient pools, and isotopic signatures

Teichberg¹,

Fox²,

Aguila³

et al. 2008

Mar. Ecol. Prog. Ser.

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“…Herbivores are able to choose their food based on palatability using cues like tissue %N (Mattson 1980, Boyer et al 2004, plant morphology (Hay 1997), the presence of structural compounds like cellulose and lignin (Cebrián & Duarte, 1998), or grazing deterrents (Hay & Fenical 1988). Augmented nutrient supply has been shown to increase tissue %N of macrophytes in Waquoit Bay (Teichberg et al 2007(Teichberg et al , 2008, and we might expect to see differences in the nutrient content of primary producers across the gradient of N loads. Uptake and assimilation of the available nutrients reduces the C to nutrient ratio of seagrass or algal tissues, making producers a better quality food source for grazers (McGlathery 1995, Hemmi & Jormalainen 2002, Boyer et al 2004, Olsen & Valiela 2010).…”

Section: Implications Of Increased N Load For C Pathways Through Bentmentioning

confidence: 99%

δ15N and δ13C reveal differences in carbon flow through estuarine benthic food webs in response to the relative availability of macroalgae and eelgrass

Olsen¹,

Fox²,

Teichberg

et al. 2011

Mar. Ecol. Prog. Ser.

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“…While nutrient assays provide useful information of short term DIN concentrations, they tend to fluctuate widely across short timescales and hence do not provide a time integrated assessment of available nitrogen. In contrast, nitrogen isotope values of rapidly growing algal indicator species (e.g., Ulva australis) provide an assessment of the available nitrogen pool over weeks (Aguiar et al 2003, Teichberg et al 2007) and can be used to identify its source (i.e., wastewater, agricultural products or atmospheric deposition; Cole et al 2004). This technique has been successfully applied to identify linkages between terrestrial catchments and estuarine habitats (e.g., McClelland and Valiela 1998, Valiela et al 2000, Bowen et al 2007 but not on open rocky coasts.…”

Section: Introductionmentioning

confidence: 99%

Land‐to‐sea connectivity: linking human‐derived terrestrial subsidies to subtidal habitat change on open rocky coasts

Gorman

Russell

Connell³

2009

Ecological Applications

115

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Transfer of CopyrightThe Author(s) shall retain the right to quote from, reprint, translate and reproduce the work, in part or in full, in any book or article he/she may later write, or in any public presentation. The Author may post the work in a publicly accessible form on his/her personal or home institution's webpages. DATE: 27 August 2012Ecological Applications, 19(5), 2009Applications, 19(5), , pp. 1114Applications, 19(5), -1126Applications, 19(5), Ó 2009 Abstract. Spatial subsidies are considered strong where differences in resource availability between donor and recipient systems are greatest. We tested whether human activities on land can increase subsidies of terrigenous nitrogen to open rocky coasts and whether these differences can predict apparent deforestation of kelp forests. We first identified landscapescale variation in the human-mediated transfer of dissolved inorganic nitrogen (DIN) from natural, agricultural, and urban catchments to temperate coasts after episodes of rainfall. Compared to natural catchments, subsidies of DIN were on average 8-407 times greater in urban catchments, and 1-63 times greater in agricultural catchments. Urban derived nitrogen was attributed to the release of sewage effluent, as delineated by d15 N isotopic values of transplanted algae. Having made this link, we then assessed whether this catchment-scale variation may account for variation in structure of subtidal habitats, particularly as related to theory of nutrient-driven shifts of habitat from perennial (i.e., canopy-forming algae) to opportunistic species (i.e., turf-forming algae). We not only detected patterns consistent with this theory, but also established that the size and total proportion of patches of turf-forming algae were greater where the ratio of donor : recipient nitrogen loads was greater (i.e., size of subsidy). An important realization was that deforestation may be more strongly related to variation in the size of subsidy rather than size of human populations, particularly among urban catchments. These data directly link the type of human activity within catchments to the modification of land-to-sea subsidies and indirectly support theory that predicts terrestrial inputs to have greater ecological effects where the disparity in resource availability between donor and recipient is exacerbated. Our evidence has been used by coastal managers to reconsider their management of coastal systems and has subsequently contributed to new water-recycling policy and initiatives.

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Nitrate reductase and glutamine synthetase activity, internal N pools, and growth of Ulva lactuca: responses to long and short-term N supply

Cited by 56 publications

References 44 publications

Macroalgal responses to experimental nutrient enrichment in shallow coastal waters: growth, internal nutrient pools, and isotopic signatures

Macroalgal responses to experimental nutrient enrichment in shallow coastal waters: growth, internal nutrient pools, and isotopic signatures

δ15N and δ13C reveal differences in carbon flow through estuarine benthic food webs in response to the relative availability of macroalgae and eelgrass

Land‐to‐sea connectivity: linking human‐derived terrestrial subsidies to subtidal habitat change on open rocky coasts

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