Nitrate reductase in cotyledons of cucumber seedlings as affected by nitrate, phytochrome and calcium

Bergareche, Carmen; Ayuso, Roberto; Masgrau, Carles; Simón, Esther

doi:10.1034/j.1399-3054.1994.910218.x

Cited by 3 publications

(8 citation statements)

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“…Sinapis alba a, c, mRNA Mohr et al (1992 [64] ) Spirodela polyrhiza a, c, mRNA Appenroth et al (1992 [3] ) Zea mays a, c, mRNA Raghuram and Sopory (1999 [79] ); Sharma et al (1994 [90] ) Curcubita maxima a, c, mRNA Bergareche et al (1994 [9] ); Oelmüller and Briggs (1990 [69] ); Rajasekhar et al (1988 [80] ) Lycopersicon esculentum a, c, mRNA Migge et al (1997 [61] ); Goud and Sharma (1994 [30] ); Becker et al (1992 [8] ) Hordeum vulgare a, c, mRNA Melzer et al (1989 [60] ); Lillo and Henriksen (1984 [45] )…”

Section: Lfrmentioning

confidence: 99%

“…Discussing the signal transduction of light-induced gene expression of NR, the effect of different photoreceptors, i.e., phytochrome versus chlorophyll (photosynthesis), should not be neglected. In one of the first papers investigating possible steps of the signal transduction of phytochrome-mediated NR gene expression, Bergareche et al (1994 [9] ) showed, in etiolated cucumber seedlings, that the effect of light could only be observed in the presence of exogenously applied Ca 2+ , and that a Ca 2+ ionophore mimicked the effect of red light. Further work to elucidate the signal transduction chain leading from phytochrome to NR gene expression was done by Sopory and co-workers (Jawaharlal Nehru University, New Delhi, India).…”

Section: Signal Transduction In Light-induced Nr Gene Expressionmentioning

confidence: 99%

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Light Regulation of Nitrate Reductase in Higher Plants: Which Photoreceptors are Involved?

Lillo

Appenroth

2001

Plant Biology

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The regulation by light of nitrate reductase (NR) in higher plants is very complex. NR is firstly regulated by transcriptional control. In etiolated plants, phytochrome is the main photoreceptor involved and its low fluence response (LFR) is the common response mode. The effect of the very low fluence response (VLFR) has been reported for one of the isoforms in Arabidopsis thaliana, NIA2. The existence of a far‐red light‐mediated high irradiance response (HIR) has been demonstrated in phytochrome A over‐expressing tobacco, confirming classical photophysiological experiments. For Lemna aequinoctialis, the existence of a red light‐mediated high irradiance response has been assumed. In green plants, the effect of phytochrome on the level of NR protein is very modest, and the effect of light is mediated via photosynthesis, most probably through biochemical signals. The molecular basis of this switch from the action of phytochromes in etiolated tissue to effects of photosynthesis in green tissue is not known. The influence of light on NR is also regulated by fast post‐translational modification. This mechanism is based on the phosphorylation/dephosphorylation of a serine residue in the hinge 1 region of NR and the subsequent Mg2+/polyamine‐dependent binding of the phosphorylated form to a 14‐3‐3 protein. Several kinases phosphorylate NR, and phosphatases not only act on NR but also dephosphorylate NR kinases. No influence of phytochrome is detectable. Instead, post‐translational modification depends on the effect of photosynthesis. Photosynthesis may exert its effect on this process via biochemical products of photosynthesis (glucose, sugar phosphates), via substrates (nitrate, NADH) and/or by influencing Ca2+ flux.

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Section: Lfrmentioning

confidence: 99%

Section: Signal Transduction In Light-induced Nr Gene Expressionmentioning

confidence: 99%

Light Regulation of Nitrate Reductase in Higher Plants: Which Photoreceptors are Involved?

Lillo

Appenroth

2001

Plant Biology

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“…This suggests that white light induces NR gene transcription indirectly through photosynthates. On the other hand, the steady state NR mRNA levels of etiolated seedlings of various species increase in response to red (Rajasekhar et al, 1988;Melzer et al, 1989;Becker et al, 1992;Bergareche et al, 1994) and blue (Melzer et al, 1989) light. In the constitutive photomorphogenic mutant copl, NRZ, along with many other light-regulated genes, is derepressed in the dark (Deng et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

A chlorate-resistant mutant defective in the regulation of nitrate reductase gene expression in Arabidopsis defines a new HY locus.

Lin

Cheng

1997

Plant Cell

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Light acts both directly as a signal and indirectly through photosynthesis to regulate the expression of genes encoding nitrate reductase (NR). Here, we report the isolation and characterization of a novel chlorate-resistant mutant that is defective in the regulation of NR gene expression. The response of NR2, but not NR1 or the gene encoding nitrate reductase (NiR), to light signals was impaired in this Arabidopsis mutant, designated cr88. In addition to NR2, the light regulation of the genes encoding the chlorophyll a/b binding protein (CAB) and the small subunit of ribulose bisphosphate carboxylase (RBCS) was also impaired in this mutant. These results suggest that the pathway through which light regulates the expression of NR2, CAB, and RBCS genes is different from those that regulate the expression of NR1 and NiR. An examination of the deetiolation process under different light spectrum showed that cr88 is defective in red light-mediated deetiolation. Complementation tests with various long hypocotyl (hy) mutants indicated that CR88 identifies a new HY locus. The possible functions of CR88 are discussed.

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“…The Ca 2+ -enhanced effect on histone phosphorylation coincides with the same stimulatory effect observed with R and FR light treatments (Figs. 1 and 2a) suggesting that Ca 2+ could be involved in this phytochrome response, as was suggested by Bergareche et al (1994) in their study of phytochrome control of NR activity in cucumber cotyledons. Fallon et al (1993) also provided evidence for the involvement of Ca 2+ -sensitive protein phosphorylation in the transduction sequence of R light signal in etiolated wheat leaf protoplasts.…”

Section: Effect Of Calcium On In Vitro Protein Phosphorylationmentioning

confidence: 76%

“…Phytochromes control the nitrate reductase (NR) activity of Cucumis sativus cotyledons only in the presence of external calcium; and calcium ionophore mimics the effect of R light (Bergareche et al, 1994). In addition, phosphoinositides and protein phosphorylation are involved in the phototransduction pathway of NR response in cucumber cotyledons (Vidal et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Protein kinase activity in Cucumis sativus cotyledons: Effect of calcium and light

et al. 2007

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Nitrate reductase in cotyledons of cucumber seedlings as affected by nitrate, phytochrome and calcium

Cited by 3 publications

References 0 publications

Light Regulation of Nitrate Reductase in Higher Plants: Which Photoreceptors are Involved?

Light Regulation of Nitrate Reductase in Higher Plants: Which Photoreceptors are Involved?

A chlorate-resistant mutant defective in the regulation of nitrate reductase gene expression in Arabidopsis defines a new HY locus.

Protein kinase activity in Cucumis sativus cotyledons: Effect of calcium and light

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