Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium

Abstract

Strain pht-3BT was isolated from a pyrene-degrading consortium of an enriched sediment from the Pacific Ocean, collected during the screening of polycyclic aromatic hydrocarbon-degrading bacteria. Cells were Gram-negative, short rods that were motile by means of flagella. Growth was observed at 0-7 % NaCl and 10-41 6C. DNA-DNA hybridization between strain pht-3B T and these reference strains revealed 55, 54, 28and 42 % DNA-DNA relatedness, respectively. The dominant fatty acids were C 19 : 0 v8c cyclo (22.6 %) and summed feature 8 (consisting of C 18 : 1 v7c and/or C 18 : 1 v6c; 60.4 %). The G+C content of the chromosomal DNA was 63 mol%. These characteristics were in good agreement with those of members of the genus Nitratireductor. According to cell morphology, physiology, fatty acid composition, 16S rRNA gene sequence analysis and DNA-DNA relatedness, the isolate belonged to the genus Nitratireductor but could be readily distinguished from recognized species of the genus. Therefore a novel species is proposed to accommodate strain pht-3B

Nitratireductor alexandrii sp. nov., from phycosphere microbiota of toxic marine dinoflagellate Alexandrium tamarense

“…At the time of writing, the genus Nitratireductor comprises eight strains with validly published names. The type strains of these species were isolated from various environmental habitats including marine denitrification system [1], seaweed [2], black beach sand [3], deep-sea water [4], pyrene-degrading consortium [5], salt lake [6] and estuary [7], as well as diatom culture [8]. Members of the genus Nitratireductor are Gram-negative, aerobic, rod-shaped or coccoid bacteria with variable motility and nitrate-reducing abilities.…”

“… § Data from original study: a , Kim et al [3]; b , Lai et al [5]; c , Labbe et al [1]; d , Jang et al [8]; e , Kang et al [2]; f , Ou et al [7]; g , Lai et al [4]; h , Yu et al [6]. …”

Genome Sequence of Nitratireductor pacificus Type Strain pht-3B

“…T (MCCC 1A01024 T , CCTCC AB 209302 T , LMG 25541 T ) was isolated from deep sea sediments of the Pacific Ocean enriched with pyrene as the sole carbon and energy source (2,5). Until now, only strains of this species have been isolated from a marine environment (http: //www.mccc.org.cn), although 49 strains of the genus Nitratireductor were present.…”

Nitratireductor lucknowense sp. nov., a novel bacterium isolated from a pesticide contaminated soil

“…The genus Nitratireductor was proposed by Labbe et al (2004) in order to accommodate Gram-negative, facultative aerobe, white-pigmented and rod shaped bacteria, isolated from a marine denitrification system, which formed a novel lineage in the family ''Phyllobacteriaceae''. At the time of writing, the genus contains six validly described species, namely Nitratireductor aquibiodomus (Labbé et al 2004), Nitratireductor basaltis (Kim et al 2009), Nitratireductor kimnyeongensis (Kang et al 2009), Nitratireductor indicus (Lai et al 2011), N. pacificus (Lai et al 2010) and Nitratireductor aquimarinus (Jang et al 2011). We have reported the bacterial diversity of several chlorinated pesticide contaminated sites and evaluated their functional capabilities for biodegradation (Manickam et al 2010).…”

“…hydrocarbon (PAH) contaminated sediment, it was found to have the capability of degrading the fourringed PAH pyrene (Lai et al 2010). The genus Nitratireductor was proposed by Labbe et al (2004) in order to accommodate Gram-negative, facultative aerobe, white-pigmented and rod shaped bacteria, isolated from a marine denitrification system, which formed a novel lineage in the family ''Phyllobacteriaceae''.…”