Nitric Oxide and Glutathione Act Synergistically to Improve PSII Activity and PSI Electron Transfer Under Chilling Stress in Cucumber Leaves

Zhou, Yang; Wang, Xiaoyu; Cui, Jun; Liu, Huiying; Cui, Huimei; Wu, Pei

doi:10.1007/s00344-023-10936-x

Cited by 4 publications

(2 citation statements)

References 59 publications

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“…GSNO/NO regulates the antioxidant system in different abiotic stress environments and improves plant resistance, suggesting that GSH participates in the NO signaling pathway to combat abiotic stresses ( Hasan et al., 2016 ). The previous study demonstrated a synergistic role of NO and GSH in enhancing PSII activity and PSI transduction in cucumber leaves exposed to low-temperature stress ( Yang et al., 2023 ). Currently, only a few studies have reported NO contributions to reducing abiotic stress-induced damage in plants via GSH ( Hasan et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide signal is required for glutathione-induced enhancement of photosynthesis in salt-stressed Solanum lycopersicum L

Cong,

Chen,

Xing

et al. 2024

Front. Plant Sci.

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Reduced glutathione (γ-glutamyl-cysteinyl-glycine, GSH), the primary non-protein sulfhydryl group in organisms, plays a pivotal role in the plant salt stress response. This study aimed to explore the impact of GSH on the photosynthetic apparatus, and carbon assimilation in tomato plants under salt stress, and then investigate the role of nitric oxide (NO) in this process. The investigation involved foliar application of 5 mM GSH, 0.1% (w/v) hemoglobin (Hb, a nitric oxide scavenger), and GSH+Hb on the endogenous NO levels, rapid chlorophyll fluorescence, enzyme activities, and gene expression related to the Calvin cycle in tomato seedlings (Solanum lycopersicum L. cv. ‘Zhongshu No. 4’) subjected short-term salt stress (100 mM NaCl) for 24, 48 and 72 hours. GSH treatment notably boosted nitrate reductase (NR) and NO synthase (NOS) activities, elevating endogenous NO signaling in salt-stressed tomato seedling leaves. It also mitigated chlorophyll fluorescence (OJIP) curve distortion and damage to the oxygen-evolving complex (OEC) induced by salt stress. Furthermore, GSH improved photosystem II (PSII) electron transfer efficiency, reduced QA- accumulation, and countered salt stress effects on photosystem I (PSI) redox properties, enhancing the light energy absorption index (PIabs). Additionally, GSH enhanced key enzyme activities in the Calvin cycle and upregulated their genes. Exogenous GSH optimized PSII energy utilization via endogenous NO, safeguarded the photosynthetic reaction center, improved photochemical and energy efficiency, and boosted carbon assimilation, ultimately enhancing net photosynthetic efficiency (Pn) in salt-stressed tomato seedling leaves. Conversely, Hb hindered Pn reduction and NO signaling under salt stress and weakened the positive effects of GSH on NO levels, photosynthetic apparatus, and carbon assimilation in tomato plants. Thus, the positive regulation of photosynthesis in tomato seedlings under salt stress by GSH requires the involvement of NO.

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Section: Introductionmentioning

confidence: 99%

Nitric oxide signal is required for glutathione-induced enhancement of photosynthesis in salt-stressed Solanum lycopersicum L

Cong,

Chen,

Xing

et al. 2024

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…GSNO/NO regulates the antioxidant system in different abiotic stress environments and improves plant resistance, suggesting that GSH participates in the NO signaling pathway to combat abiotic stresses (Hasan et al, 2016). The previous study demonstrated a synergistic role of NO and GSH in enhancing PSII activity and PSI transduction in cucumber leaves exposed to low-temperature stress (Yang et al, 2023). Currently, only a few studies have reported NO contributions to reducing abiotic stress-induced damage in plants via GSH (Hasan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Exogenous nitric oxide treatment delays the senescence of postharvest mung bean sprouts by regulating ascorbic acid metabolism

Wang,

Qiu,

Zhang

et al. 2024

J Sci Food Agric

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BACKGROUNDThis study evaluated the effects of nitric oxide (NO) treatment on ascorbic acid (AsA) metabolism and mung bean sprout quality. It examined changes in the AsA content, enzyme activity associated with AsA metabolism, antioxidant capacity, cell membrane composition, and cellular structure to clarify the effects of NO on mung bean sprouts.RESULTSNitric oxide treatment preserved mung bean sprout quality by enhancing significantly the activity of enzymes involved in the l‐galactose pathway (including guanosine diphosphate (GDP)glutathione (‐d‐mannose pyrophosphorylase, GDP‐mannose‐3′,5′‐epimerase, GDP‐l‐galactose phosphorylase, l‐galactose‐1‐phosphate phosphatase, l‐galactose dehydrogenase, and l‐galactose‐1,4‐lactone dehydrogenase) and the AsA‐glutathione (GSH)(Beijing Solarbio Science and Technology Co.,Ltd., Beijing, China) cycle (including ascorbate peroxidase, ascorbic acid oxidase, glutathione reductase, dehydroascorbate reductase, and monodehydroascorbate reductase) during the germination and storage stage. Increased enzyme activity led to an increase in AsA content and enhanced antioxidant capacity, and reduced the membrane lipid damage in mung bean sprouts. This was demonstrated by higher levels of DPPH radical scavenging capacity, unsaturated fatty acids and phospholipids, along with lower levels of hydrogen peroxide, superoxide anions, and malondiadehyde, in NO‐treated mung bean sprouts. Scanning electron microscopy also revealed that NO treatment maintained the integrity of the cellular structure of the mung bean sprouts.CONCLUSIONNitric oxide accelerates AsA metabolism effectively by regulating the biosynthesis and regeneration of AsA in mung bean sprouts. These changes increased AsA levels, alleviated membrane lipid damage, delayed senescence, and maintained the quality of mung bean sprouts during storage. © 2024 Society of Chemical Industry.

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Nitric Oxide and Glutathione Act Synergistically to Improve PSII Activity and PSI Electron Transfer Under Chilling Stress in Cucumber Leaves

Cited by 4 publications

References 59 publications

Nitric oxide signal is required for glutathione-induced enhancement of photosynthesis in salt-stressed Solanum lycopersicum L

Nitric oxide signal is required for glutathione-induced enhancement of photosynthesis in salt-stressed Solanum lycopersicum L

Table_1.xlsx

Exogenous nitric oxide treatment delays the senescence of postharvest mung bean sprouts by regulating ascorbic acid metabolism

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