Nitric oxide elicitation for secondary metabolite production in cultured plant cells

Abstract

Nitric oxide (NO) is an important signal molecule in stress responses. Accumulation of secondary metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. NO has been reported to play important roles in elicitor-induced secondary metabolite production in tissue and cell cultures of medicinal plants. Better understanding of NO role in the biosynthesis of such metabolites is very important for optimizing the commercial production of those pharmaceutically significant secondary metabolites. This paper summarizes progress made on several aspects of NO signal leading to the production of plant secondary metabolites, including various abiotic and biotic elicitors that induce NO production, elicitor-triggered NO generation cascades, the impact of NO on growth development and programmed cell death in medicinal plants, and NO-mediated regulation of the biosynthetic pathways of such metabolites. Cross-talks among NO signaling and reactive oxygen species, salicylic acid, and jasmonic acid are discussed. Some perspectives on the application of NO donors for induction of the secondary metabolite accumulation in plant cultures are also presented.

Fungi Treated with Small Chemicals Exhibit Increased Antimicrobial Activity against Facultative Bacterial and Yeast Pathogens

“…It has been described that H. koningii produces various koninginins, which display a wide range of bioactivities including antimicrobial activity against gram negative bacteria [37, 38]. Our observation that also the addition of NO increases the antimicrobial activity of H. koningii (and also in P. miczynskii ) supports the hypothesis that the phytohormone NO influences SM production also in fungi, not only in plants [26, 27, 39]. At present, it is unknown if NO influences chromatin structure in fungi; therefore, further research is needed to study the role of NO in the regulation of secondary metabolite production.…”

Nanoparticles Alter Secondary Metabolism in Plants via ROS Burst

“…BRs, the steroidal phytohormones that play important role in plant growth, secondary metabolite accumulation, stress responses and adaptation (Çoban and Göktürk Baydar, 2016) could ameliorate ZnONP-induced oxidative stress by improving antioxidant potential and redox homeostasis in tomato seedlings (Li et al, 2016). NO, another universal signaling molecule that plays a central role in secondary metabolite production in plant cells (Zhang et al, 2012; Zeng et al, 2014), is also involved in plant–NP interactions. For instance, AgNP-induced phytotoxicity could be alleviated by NO in P. sativum seedlings (Tripathi et al, 2017).…”

Paclitaxel production and PAL activity in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene elicited with nitric oxide and methyl jasmonate

“…Indications that NO is involved in signaling defense responses during plant-pathogen interactions are well documented (Sudha and Ravishankar 2002;Arasimowicz and Floryszak-Wieczorek 2007;Zhang et al 2012;Scheler et al 2013;Yu et al 2014;Trapet et al 2014). Induced NO generation has also been reported, using various fungal elicitors, which mediate the accumulation of secondary metabolites belonging to diverse chemical groups .…”