Nitric oxide modulates specific steps of auxin-induced adventitious rooting in sunflower

Yadav, Sunita; David, Anisha; Bhatla, Satish C.

doi:10.4161/psb.5.10.12159

Cited by 21 publications

(12 citation statements)

References 24 publications

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“…In addition, MT altered the expression of a large number of auxin-related genes. The polar localization of PIN proteins is responsible for auxin transport and disruption, which directly affect IAA transport to regulate the ARF process (Yadav et al, 2010 ). A previous study indicated that PIN1 promoted the auxin lateral efflux from the hypocotyl vasculature to the pericycle founder cells in ARF (Della Rovere et al, 2013 ), which suggests that MT-induced PIN1 expression was beneficial for ARF in our study (Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

Promoting Roles of Melatonin in Adventitious Root Development of Solanum lycopersicum L. by Regulating Auxin and Nitric Oxide Signaling

et al. 2016

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Melatonin (MT) plays integral roles in regulating several biological processes including plant growth, seed germination, flowering, senescence, and stress responses. This study investigated the effects of MT on adventitious root formation (ARF) of de-rooted tomato seedlings. Exogenous MT positively or negatively influenced ARF, which was dependent on the concentration of MT application. In the present experiment, 50 μM MT showed the best effect on inducing ARF. Interestingly, exogenous MT promoted the accumulation of endogenous nitric oxide (NO) by down-regulating the expression of S-nitrosoglutathione reductase (GSNOR). To determine the interaction of MT and NO in ARF, MT synthesis inhibitor p-chlorophenylalanine, NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt as well as GSNOR-overexpression plants with low NO levels were used. The function of MT was removed by NO scavenger or GSNOR-overexpression plants. However, application of MT synthesis inhibitor did little to abolish the function of NO. These results indicate that NO, as a downstream signal, was involved in the MT-induced ARF. Concentrations of indole-3-acetic acid and indole-3-butyric acid, as well as the expression of several genes related to the auxin signaling pathway (PIN1, PIN3, PIN7, IAA19, and IAA24), showed that MT influenced auxin transport and signal transduction as well as auxin accumulation through the NO signaling pathway. Collectively, these strongly suggest that elevated NO levels resulting from inhibited GSNOR activity and auxin signaling were involved in the MT-induced ARF in tomato plants. This can be applied in basic research and breeding.

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Section: Discussionmentioning

confidence: 99%

Promoting Roles of Melatonin in Adventitious Root Development of Solanum lycopersicum L. by Regulating Auxin and Nitric Oxide Signaling

et al. 2016

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“…In these studies a co-action of NO and auxin has often become apparent, with NO being induced by auxin. In sunflower, it was suggested that NO could participate with auxin in adventitious root initiation and expression (extension), whereas induction would depend only on auxin (Yadav et al, 2010). Studies on AR of Tagetes erecta (marigold; Liao et al, 2009), Vigna radiata (mung bean; Li and Xue, 2010), and Chrysanthemum (Liao et al, 2010) have suggested that H 2 O 2 and NO may act together, possibly as parallel independent pathways dependent on Ca 2+ , converging on the activation of MAPK cascades leading to AR.…”

Section: Phytohormonal Balances: the Seesaw Of Promotion Versus Reprementioning

confidence: 99%

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Costa¹,

Almeida²,

Ruedell³

et al. 2013

Front. Plant Sci.

311

280

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Adventitious rooting (AR) is a multifactorial response leading to new roots at the base of stem cuttings, and the establishment of a complete and autonomous plant. AR has two main phases: (a) induction, with a requirement for higher auxin concentration; (b) formation, inhibited by high auxin and in which anatomical changes take place. The first stages of this process in severed organs necessarily include wounding and water stress responses which may trigger hormonal changes that contribute to reprogram target cells that are competent to respond to rooting stimuli. At severance, the roles of jasmonate and abscisic acid are critical for wound response and perhaps sink strength establishment, although their negative roles on the cell cycle may inhibit root induction. Strigolactones may also inhibit AR. A reduced concentration of cytokinins in cuttings results from the separation of the root system, whose tips are a relevant source of these root induction inhibitors. The combined increased accumulation of basipetally transported auxins from the shoot apex at the cutting base is often sufficient for AR in easy-to-root species. The role of peroxidases and phenolic compounds in auxin catabolism may be critical at these early stages right after wounding. The events leading to AR strongly depend on mother plant nutritional status, both in terms of minerals and carbohydrates, as well as on sink establishment at cutting bases. Auxins play a central role in AR. Auxin transporters control auxin canalization to target cells. There, auxins act primarily through selective proteolysis and cell wall loosening, via their receptor proteins TIR1 (transport inhibitor response 1) and ABP1 (Auxin-Binding Protein 1). A complex microRNA circuitry is involved in the control of auxin response factors essential for gene expression in AR. After root establishment, new hormonal controls take place, with auxins being required at lower concentrations for root meristem maintenance and cytokinins needed for root tissue differentiation.

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“…34 The process of AR formation consists of 3 physiologically interdependent phases -induction, initiation and extension. 34,35 AR formation involves the interaction of environmental and endogenous factors among which the phytohormone auxin plays an important role. The three phases of AR formation that is induction, initiation and extension are regulated by alterations in the endogenous level of auxin suggesting its central role.…”

Section: Introductionmentioning

confidence: 99%

“…58 Using various pharmacological agents, a probable signaling cascade (linking NO, cyclic GMP and mitogen-activated protein kinases) for auxin-induced AR formation has been proposed in cucumber seedlings. [59][60][61] Yadav et al, 35 reported NO modulation during initiation and extension phases of auxininduced AR formation in sunflower seedlings. Exogenous supply of NO donors-sodium nitroprusside (SNP) and S-nitroso N-acetyl penicillamine (SNAP) to cucumber explants mimicks the effect of IAA, leading to AR formation.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide mediates strigolactone signaling in auxin and ethylene-sensitive lateral root formation in sunflower seedlings

Bharti

Bhatla²

2015

Plant Signaling & Behavior

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Strigolactones (SLs) play significant role in shaping root architecture whereby auxin-SL crosstalk has been observed in SL-mediated responses of primary root elongation, lateral root formation and adventitious root (AR) initiation. Whereas GR24 (a synthetic strigolactone) inhibits LR and AR formation, the effect of SL biosynthesis inhibitor (fluridone) is just the opposite (root proliferation). Naphthylphthalamic acid (NPA) leads to LR proliferation but completely inhibits AR development. The diffusive distribution of PIN1 in the provascular cells in the differentiating zone of the roots in response to GR24, fluridone or NPA treatments further indicates the involvement of localized auxin accumulation in LR development responses. Inhibition of LR formation by GR24 treatment coincides with inhibition of ACC synthase activity. Profuse LR development by fluridone and NPA treatments correlates with enhanced [Ca 2C ] cyt in the apical region and differentiating zones of LR, indicating a critical role of [Ca 2C ] in LR development in response to the coordinated action of auxins, ethylene and SLs. Significant enhancement of carotenoid cleavage dioxygenase (CCD) activity (enzyme responsible for SL biosynthesis) in tissue homogenates in presence of cPTIO (NO scavenger) indicates the role of endogenous NO as a negative modulator of CCD activity. Differences in the spatial distribution of NO in the primary and lateral roots further highlight the involvement of NO in SL-modulated root morphogenesis in sunflower seedlings. Present work provides new report on the negative modulation of SL biosynthesis through modulation of CCD activity by endogenous nitric oxide during SL-modulated LR development.

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Nitric oxide modulates specific steps of auxin-induced adventitious rooting in sunflower

Cited by 21 publications

References 24 publications

Promoting Roles of Melatonin in Adventitious Root Development of Solanum lycopersicum L. by Regulating Auxin and Nitric Oxide Signaling

Promoting Roles of Melatonin in Adventitious Root Development of Solanum lycopersicum L. by Regulating Auxin and Nitric Oxide Signaling

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Nitric oxide mediates strigolactone signaling in auxin and ethylene-sensitive lateral root formation in sunflower seedlings

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