Nitric Oxide Promotes Infectious Bone Resorption by Enhancing Cytokine-Stimulated Interstitial Collagenase Synthesis in Osteoblasts

Lin, Sze‐Kwan; Kok, Sang‐Heng; Kuo, Mark Yen‐Ping; Lee, Ming‐Shu; Wang, Chih-Chiang; Lan, Wan–Hong; Hsiao, Michael; Goldring, Steven R.; Hong, Chuang‐Ye

doi:10.1359/jbmr.2003.18.1.39

Cited by 53 publications

(52 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Another study preformed by Uchida et al (2000) in mouse primary osteoblasts showed elevated mRNA levels of MMP-3,-13 after TNF-induction. Rifas et al (1994) reported that MMP-2 synthesis in normal human osteoblasts is not regulated by bone resorbing agents and in contrast to our Wndings, he reported that MMP-9 is not regulated as well (Fujisaki et al 2006;Lin et al 2003;Rifas et al 1994;Uchida et al 2000).…”

Section: Discussioncontrasting

confidence: 99%

See 1 more Smart Citation

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

David

Reznick

Srouji

et al. 2008

Histochem Cell Biol

View full text Add to dashboard Cite

An intimate interplay exists between the bone and the immune system, which has been recently termed osteoimmunology. The activity of immune cells affects the intrinsic balance of bone mineralization and resorption carried out by the opposing actions of osteoblasts and osteoclasts. The aim of this study was to determine the possible interaction between inflammatory-induced conditions and matrix metalloproteinases-2,-9 (MMP-2,-9) synthesis and secretion by bone marrow-derived osteoprogenitor cells during advanced stages of osteogenesis. Rat bone marrow-derived mesenchymal stem cells (MSCs) were cultured in the presence of osteogenic supplements in order to direct the cells towards the osteogenic differentiation lineage. At the late stages of osteogenesis, assessed by histochemistry, immunohistochemistry and RT-PCR, cultures were exposed to pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 alpha (IL-1 alpha). Biochemical, histochemical and molecular biology techniques were used to discern the influence of pro-inflammatory cytokines on MMP-2,-9 synthesis and secretion. Results indicated that MMP-9 synthesis and secretion were significantly induced after exposure to the cytokines (TNF-alpha, IL-1 alpha) treatment, while MMP-2 levels remained unchanged. These results indicate that in response to inflammatory processes, osteoblasts, in addition to osteoclasts, can also be involved and contribute to the process of active bone resorption by secretion and activation of MMPs.

show abstract

Section: Discussioncontrasting

confidence: 99%

“…Similarly, Lin et al (2003) showed that TNFincreased MMP-1 mRNA levels in mouse osteoblasts. Another study preformed by Uchida et al (2000) in mouse primary osteoblasts showed elevated mRNA levels of MMP-3,-13 after TNF-induction.…”

Section: Discussionmentioning

confidence: 92%

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

David

Reznick

Srouji

et al. 2008

Histochem Cell Biol

View full text Add to dashboard Cite

show abstract

“…Both iNOS and eNOS have been found to play roles in osteoblast differentiation. High concentrations of NO release resulting from pathological iNOS expression is thought to promote bone resorption and bone formation (Lin et al, 2003;Chae et al, 1997) by reducing osteoblast activity (Hukkanen et al, 1995). In contrast, during development eNOS is constitutively expressed by osteoblast-like cells.…”

Section: Introductionmentioning

confidence: 99%

NO/beta-catenin crosstalk modulates primitive streak formation prior to embryonic stem cell osteogenic differentiation

Ding

Keller

Martinez

et al. 2012

Journal of Cell Science

View full text Add to dashboard Cite

SummaryNitric oxide (NO) has been shown to play a crucial role in bone formation in vivo. We sought to determine the temporal effect of NO on murine embryonic stem cells (ESCs) under culture conditions that promote osteogenesis. Expression profiles of NO pathway members and osteoblast-specific markers were analyzed using appropriate assays. We found that NO was supportive of osteogenesis specifically during an early phase of in vitro development (days 3-5). Furthermore, ESCs stably overexpressing the inducible NO synthase showed accelerated and enhanced osteogenesis in vitro and in bone explant cultures. To determine the role of NO in early lineage commitment, a stage in ESC differentiation equivalent to primitive streak formation in vivo, ESCs were transfected with a T-brachyury-GFP reporter. Expression levels of T-brachyury and one of its upstream regulators, b-catenin, the major effector in the canonical Wnt pathway, were responsive to NO levels in differentiating primitive streak-like cells. Our results indicate that NO may be involved in early differentiation through regulation of b-catenin and T-brachyury, controlling the specification of primitive-streak-like cells, which may continue through differentiation to later become osteoblasts.

show abstract

“…Some studies have shown that NO induces both expression and activation of MMP-1 in chondrocyte cultures, as well as induces MMP-1 and MMP-2 expression and activity in fibroblasts (Lin et al, 2003;Choe et al, 2003). Therefore, iNOS expression in mouse skin was evaluated by immunohistochemistry and the results show an agreement with this supposition, as the skin from group 1 mice treated with vehicle or 10% L-arginine had a lower amount of collagen fibers than skin treated with 15% L-arginine, which also did not exhibit iNOS expression in the epithelium or showed very little iNOS expression in the connective tissue.…”

Section: Discussionmentioning

confidence: 99%

The in vivo effect of L-arginine on skin elasticity in mice

Souza

Oliveira

Andrade

et al. 2017

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

The human skin aging process is a complex mechanism that can be induced both by intrinsic and extrinsic factors. Observations include a decrease in the biosynthetic and proliferative capacity of cells, increased expression of matrix metalloproteinases, reduction in collagen type I expression, and the progressive disappearance of elastic tissue in the papillary dermis. L-arginine, the substrate of nitric oxide synthesis, is involved in angiogenesis and cell proliferation, as well as an indirect precursor of collagen synthesis via the proline pathway. The aim of this study was to examine the tensile strength, histology, and immunohistochemistry of female and male mice skin receiving different concentrations of topically applied L-arginine, in order to evaluate the possibility of using L-arginine as an active cosmetic ingredient in antiaging products. The results suggest that the application of L-arginine improves the mechanical resistance of skin from older female mice (20 weeks old) and promotes the formation of a larger amount of collagen and elastic fibers in the skin when applied at a concentration of 15%.

show abstract

Nitric Oxide Promotes Infectious Bone Resorption by Enhancing Cytokine-Stimulated Interstitial Collagenase Synthesis in Osteoblasts

Cited by 53 publications

References 35 publications

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

Exposure to pro-inflammatory cytokines upregulates MMP-9 synthesis by mesenchymal stem cells-derived osteoprogenitors

NO/beta-catenin crosstalk modulates primitive streak formation prior to embryonic stem cell osteogenic differentiation

The in vivo effect of L-arginine on skin elasticity in mice

Contact Info

Product

Resources

About