Nitric oxide synthase distribution in the rat intestine: A histochemical analysis

Nichols, Kim; Staines, William; Krantis, Anthony

doi:10.1016/0016-5085(93)91060-u

Cited by 111 publications

(55 citation statements)

References 31 publications

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“…It has been demonstrated that the specificity and sensitivity of NADPH-diaphorase histochernistry is greatly influenced by slight modifications in fixation and histochemical procedures. However, the method used in the present study (WARD et al, 1992b;YOUNG et al, 1992;NICHOLS et al, 1993) is widely accepted as one of the most specific methods for the detection of NADPH-diaphorase associated with NOSs. Hence, those NADPH-diaphorase positive nerves demonstrated in the present study probably contain NOSs.…”

Section: Nos and Nadph-diaphorasementioning

confidence: 99%

“…Procedures of histochemical NADPH-diaphorase reaction have been described by previous authors (HOPE and VINCENT, 1989;WARD et al, 1992b;YOUNG et al, 1992;NICHOLS et al, 1993;LI and FURNESS, 1993). Briefly, pieces of the gut containing the end-ileum, cecum and proximal colon were filled out with warm (about 3TC) Ringers solution injected into the lumen, making the rising intraluminal pressure act on all parts of the circumference equally.…”

Section: Nadph-diaphorase Histochemistrymentioning

confidence: 99%

See 1 more Smart Citation

Nitrinergic Nerves Controlling Pacemaker Activities of the Inner Sublayer (P-layer) in the Canine Proximal Colon Circular Muscles.

Nahar

Chowdhury

Tokuno

et al. 1996

Archives of Histology and Cytology

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Section: Nos and Nadph-diaphorasementioning

confidence: 99%

Section: Nadph-diaphorase Histochemistrymentioning

confidence: 99%

Nitrinergic Nerves Controlling Pacemaker Activities of the Inner Sublayer (P-layer) in the Canine Proximal Colon Circular Muscles.

Nahar

Chowdhury

Tokuno

et al. 1996

Archives of Histology and Cytology

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“…Fresh tumor tissue was fixed with modified Zamboni's fixative 43 for 2 hours. The fixative was removed with ice-cold 100 mmol/L sodium phosphate buffer, pH 7.2 (PB), and the tumor fragment was stored in a 1:1 mixture of 10% sucrose, 3% Triton X-100 in PB for a minimum of 24 hours.…”

Section: Histochemical Assays For Detecting Tumorinfiltrating Host Cellsmentioning

confidence: 99%

Neutrophils, Nitric Oxide Synthase, and Mutations in the Mutatect Murine Tumor Model

Sandhu

Privora

Wenckebach

et al. 2000

The American Journal of Pathology

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Mutatect MN-11 is a tumor line that can be grown subcutaneously in syngeneic C57BL/6 mice. The frequency of spontaneously arising mutants at the hypoxanthine phosphoribosyltransferase (Hprt) locus was observed to be elevated as a result of in vivo growth. The objective of the present study was to identify factors in the tumor microenvironment that might explain this increase in mutant frequency (MF). When tumors were examined histologically, neutrophils were found to be the predominant infiltrating cell type. Quantitative estimates of the number of neutrophils and MF of tumors in different animals revealed a statistically significant correlation (r ‫؍‬ 0.63, P < 0.0001). Immunohistochemical analysis for inducible nitric oxide synthase (iNOS) demonstrated its presence, mainly in neutrophils. Biochemical analysis of tumor homogenates for nitric oxide synthase (NOS) activity indicated a statistically significant correlation with MF (r ‫؍‬ 0.77, P < 0.0001). Nitrotyrosine was detected throughout the tumor immunohistochemically; both cytoplasmic and nuclear staining was seen. To increase the number of infiltrating neutrophils, tumors were injected with chemoattractant interleukin-8 and prostaglandin E2. This produced a statistically significant increase in neutrophil content (P ‫؍‬ 0.005) and MF (P ‫؍‬ 0.0002). As in control MN-11 tumors, neutrophil content and MF were strongly correlated (r ‫؍‬ 0.63, P ‫؍‬ 0.003). Because neutrophils are a potential source of genotoxic reactive oxygen and/or nitrogen species, our results support the notion that these tumor-infiltrating cells may be mutagenic and contribute to the burden of genetic abnormalities associated with tumor progression.

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“…Endogenous NO derives from enzymatic conversion of L-arginine through NO-synthase, a family of isoenzymes, the NO-generating activity of which can be inhibited stereospecifically by L-but not by D-arginine analogues (Ignarro, 1991). In the gut, NO-synthase activity has been found throughout the intestinal wall in enteric neurones (Aimi et al, 1993), enteric blood vessels (Nichols et al, 1993) and enterocytes (M'Rabet-Touil et al, 1993). Intes-tinal NO can furthermore be produced by neutrophils (McCall et al, 1989), macrophages (Hibbs et al, 1988) and mast cells (Salvemini et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Significance of nitric oxide in the stimulation of intestinal fluid absorption in the rat jejunum in vivo

Schirgi‐Degen

Beubler

1995

British J Pharmacology

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1 The effects of inhibiting nitric oxide (NO)-synthase on fluid transport, mucosal cyclic GMP and cyclic AMP levels and intraluminal prostaglandin E2 (PGE2)-release were studied in a model of ligated jejunal loops of anaesthetized rats in vivo. Experiments were performed under basal conditions as well as under conditions, when net fluid secretion was induced by Escherichia coli heat stable enterotoxin a (E. coli STa) or PGE2. 2 Intravenous infusion of the NO-synthase inhibitor N0-nitro-L-arginine methyl ester (L-NAME, 0.25-50mgrkg-', 45min) dose-dependently reversed net fluid absorption to net secretion, whereas infusion of D-NAME, the inactive enantiomer of L-NAME, in corresponding doses did not influence net fluid transport. Nw-nitro-L-arginine (L-NOARG, 25 mg kg-'), another NO-synthase inhibitor, also elicited net secretion of fluid. -3 L-NAME (25 mg kg ')-induced net fluid secretion was reversed to net absorption by infusion of L-arginine (400 mg kg') or sodium nitroprusside (1 mg kg-') and s.c. administration of indomethacin (10 mg kg-'). Hexamethonium (1 mg kg-', s.c.), a ganglionic blocker and granisetron (100 gsg kg-', s.c.), a 5-HT3-receptor antagonist, did not influence L-NAME-induced net secretion. 4 Net fluid secretion induced by intraluminal instillation of E. coli STa (10 units ml-') was enhanced by infusion of L-NAME (25mgkg-') and was inhibited by infusion of L-arginine (400mgkg-') and sodium nitroprusside (1 mg kg-'). D-Arginine (400 mg kg-') did not influence E. coli STa-induced fluid secretion. Likewise, net fluid secretion induced by i.a. infusion of PGE2 (79 ng ml-, 30 min) was enhanced by infusion of L-NAME and was inhibited by L-arginine and sodium nitroprusside. D-Arginine (400 mg kg-') did not influence PGE2-induced fluid secretion. 5 PGE2 levels in intraluminal fluid were not elevated after infusion of L-NAME (25mgkg-') compared to controls. 6 Mucosal cyclic GMP and cyclic AMP levels after L-NAME-treatment were not different from control values. 7 These results indicate that nitric oxide plays an important role in the regulation of intestinal fluid transport. The data suggest a nitric oxide-dependent proabsorptive tone in the intestine, which possibly involves the enteric nervous system and suppression of prostaglandin formation. This proabsorptive tone also may downregulate fluid secretion induced by E. coli STa or PGE2.

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Nitric oxide synthase distribution in the rat intestine: A histochemical analysis

Cited by 111 publications

References 31 publications

Nitrinergic Nerves Controlling Pacemaker Activities of the Inner Sublayer (P-layer) in the Canine Proximal Colon Circular Muscles.

Nitrinergic Nerves Controlling Pacemaker Activities of the Inner Sublayer (P-layer) in the Canine Proximal Colon Circular Muscles.

Neutrophils, Nitric Oxide Synthase, and Mutations in the Mutatect Murine Tumor Model

Significance of nitric oxide in the stimulation of intestinal fluid absorption in the rat jejunum in vivo

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