Nitric oxide within periaqueductal gray modulates glutamatergic neurotransmission and cardiovascular responses during mechanical and thermal stimuli

Ishide, Takeshi; Amer, Ahmed; Maher, Timothy J.; Ally, Ahmmed

doi:10.1016/j.neures.2004.10.003

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…The air jet stress model employed in this study is complex, involving components that are both physical (restraint, and perhaps cold owing to the dissipation of body heat produced by a constant stream of air) and psychological. Our findings provide no insight as to whether neurons in the l/dlPAG contribute more to the response to the physiological or psychological components of the model, but previous reports suggest a dominant role for the former (17,18,25,51). Nevertheless, this, as well as the exact placement and role of these neurons in the relevant stress-related forebrain circuitry, particularly with regard to the DMH, are yet to be fully elucidated.…”

Section: Perspectives and Significancecontrasting

confidence: 65%

Microinjection of muscimol into the periaqueductal gray suppresses cardiovascular and neuroendocrine response to air jet stress in conscious rats

Menezes¹,

Zaretsky²,

Sarkar³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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Section: Perspectives and Significancecontrasting

confidence: 65%

Microinjection of muscimol into the periaqueductal gray suppresses cardiovascular and neuroendocrine response to air jet stress in conscious rats

Menezes¹,

Zaretsky²,

Sarkar³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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“…This excitatory amino acid and its receptors have been identified in the ARC and vlPAG (Albin et al, 1990;Eyigor et al, 2001;Ishide et al, 2005;Guo and Longhurst, 2007;Kiss et al, 2005). Vesicular glutamate transporters (VGLUTs) specifically transport glutamate into vesicles of neurons, and thus offer a unique marker to distinctively identify neurons that use glutamate as a neurotransmitter (Fremeau et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, glutamatergic neurons and receptors are present in the ARC and vlPAG (Eyigor et al, 2001;Ishide et al, 2005;Guo and Longhurst, 2007;Kiss et al, 2005). In this regard, we have noted that ARC neurons activated by EA at the P5-P6 acupoints, as identified by their expression of c-Fos, contain vesicular glutamate transporter 3 (VGLUT3), a marker for glutamate (Guo and Longhurst, 2007;Guo et al, 2004;Noh et al, 2010;Seal et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Activation of reciprocal pathways between arcuate nucleus and ventrolateral periaqueductal gray during electroacupuncture: Involvement of VGLUT3

Guo¹,

Longhurst²

2010

Brain Research

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Electroacupuncture (EA) at the Jianshi-Neiguan acupoints (P5-P6, overlying the median nerve) attenuates sympathoexcitatory responses through activation of the arcuate nucleus (ARC) and ventrolateral periaqueductal gray (vlPAG). Activation of the ARC or vlPAG respectively leads to neuronal excitation of the both nuclei during EA. However, direct projections between these two nuclei that could participate in central neural processing during EA have not been identified. The vesicular glutamate transporter 3 (VGLUT3) marks glutamatergic neurons. Thus, the present study evaluated direct neuronal projections between the ARC and vlPAG during EA, focusing on neurons containing VGLUT3. Seven to ten days after unilateral microinjection of a rodamineconjugated microsphere retrograde tracer (100 nl) into the vlPAG or ARC, rats were subjected to EA or served as a sham-operated control. Low frequency (2 Hz) EA was performed bilaterally for 30 min at the P5-P6 acupoints. Perikarya containing the microsphere tracer were found in the ARC and vlPAG of both groups. Compared to controls (needle placement without electrical stimulation), c-Fos immunoreactivity and neurons double-labeled with c-Fos, an immediate early gene and the tracer were increased significantly in the ARC and vlPAG of EA-treated rats (both P<0.01). Moreover, some neurons were triple-labeled with c-Fos, the retrograde tracer and VGLUT3 in the two nuclei following EA stimulation (P<0.01, both nuclei). These results suggest that direct reciprocal projections between the ARC and vlPAG are available to participate in prolonged modulation by EA of sympathetic activity and that VGLUT3-containing neurons are an important neuronal phenotype involved in this process.

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“…The PAG plays a pivotal role in modulating nociceptive impulses and electrical stimulation of the dorsolateral and ventrolateral PAG, which produces analgesia [49][50][51]. In addition, the dorsolateral and ventrolateral PAG contain a column of NOScontaining cells, which may release NO that could participate in the inhibitory modulation of pain [52][53][54]. NO might also promote the release of serotonin, an important neurotransmitter involved in the inhibition of nociceptive impulses in the dorsal horn of the spinal cord [55].…”

Section: Discussionmentioning

confidence: 99%

The Nitric oxide/_CGMP/KATP pathway mediates systemic and central antinociception induced by resistance exercise in rats

Galdino

Xavier

Almeida

et al. 2014

International Journal of Neuroscience

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Resistance exercise (RE) is characterized to increase strength, tone, mass, and/or muscular endurance and also for produces many beneficial effects, such as blood pressure and osteoporosis reduction, diabetes mellitus control, and analgesia. However, few studies have investigated endogenous mechanisms involved in the RE-induced analgesia. Thus, the aim of this study was evaluate the role of the NO/CGMP/KATP pathway in the antinociception induced by RE. Wistar rats were submitted to acute RE in a weight-lifting model. The nociceptive threshold was measured by mechanical nociceptive test (paw-withdrawal). To investigate the involvement of the NO/CGMP/KATP pathway the following nitric oxide synthase (NOS) non-specific and specific inhibitors were used: N-nitro-l-arginine (NOArg), Aminoguanidine, N5-(1-Iminoethyl)-l-ornithine dihydrocloride (l-NIO), Nω-Propyl-l-arginine (l-NPA); guanylyl cyclase inhibitor, 1H-[1,2,4]oxidiazolo[4,3-a]quinoxalin-1-one (ODQ); and KATP channel blocker, Glybenclamide; all administered subcutaneously, intrathecally and intracerebroventricularly. Plasma and cerebrospinal fluid (CSF) nitrite levels were determined by spectrophotometry. The RE protocol produced antinociception, which was significantly reversed by NOS specific and unspecific inhibitors, guanylyl cyclase inhibitor (ODQ) and KATP channel blocker (Glybenclamide). RE was also responsible for increasing nitrite levels in both plasma and CSF. These finding suggest that the NO/CGMP/KATP pathway participates in antinociception induced by RE.

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Nitric oxide within periaqueductal gray modulates glutamatergic neurotransmission and cardiovascular responses during mechanical and thermal stimuli

Cited by 17 publications

References 35 publications

Microinjection of muscimol into the periaqueductal gray suppresses cardiovascular and neuroendocrine response to air jet stress in conscious rats

Microinjection of muscimol into the periaqueductal gray suppresses cardiovascular and neuroendocrine response to air jet stress in conscious rats

Activation of reciprocal pathways between arcuate nucleus and ventrolateral periaqueductal gray during electroacupuncture: Involvement of VGLUT3

The Nitric oxide/_CGMP/KATP pathway mediates systemic and central antinociception induced by resistance exercise in rats

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Nitric oxide within periaqueductal gray modulates glutamatergic neurotransmission and cardiovascular responses during mechanical and thermal stimuli

Cited by 17 publications

References 35 publications

Microinjection of muscimol into the periaqueductal gray suppresses cardiovascular and neuroendocrine response to air jet stress in conscious rats

Microinjection of muscimol into the periaqueductal gray suppresses cardiovascular and neuroendocrine response to air jet stress in conscious rats

Activation of reciprocal pathways between arcuate nucleus and ventrolateral periaqueductal gray during electroacupuncture: Involvement of VGLUT3

The Nitric oxide/CGMP/KATP pathway mediates systemic and central antinociception induced by resistance exercise in rats

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The Nitric oxide/_CGMP/KATP pathway mediates systemic and central antinociception induced by resistance exercise in rats