Nitrile hydratase from Rhodococcus erythropolis: Metabolization of steroidal compounds with a nitrile group

The effects of storage time and temperature on the stability of salivary phosphatases, transaminases and dehydrogenase

“…Storage time and temperature, and oral health condition are some of the factors that may influence the stability of saliva components, thus being sources of errors in laboratory determinations. Storage and temperature are factors that influence steroid hormone concentrations because of their degradation by the action of salivary enzymes and the decomposing action of oral microorganisms (Kaufmann et al, 1999). A large range of errors in the measurement of testosterone and estradiol occur when saliva is stored at temperatures above −20°C (Toone et al, 2013).…”

Nitrile-Degrading Bacteria Isolated from Compost

“…Similarity searches for putative genes involved in nitrile-degradation revealed two genes (BKP42_65670 and BKP42_65680), coding for one of the two putative NHase subunits. These enzymes are known to be responsible for nitrile degradation in various R. erythropolis strains (Kaufmann et al, 1999;Brandão et al, 2003;Vejvoda et al, 2007;Kamble et al, 2013). The deduced protein sequences showed highest identity to the alpha subunit of Rhodococcus sp.…”

Identification of bioactive products from environmental transformation of steroids

“…[90][91] Both of these applications rely upon daily doses of ~1-2 mg of active compound. [91][92] Also, metabolism of DNG in microbially active systems 93 has yielded metabolites with an aromatic A-ring, a structural feature typically resulting in significant estrogenic activity. This suggests that other transformation mechanisms, such as photolysis, may also form estrogenic transformation products.…”

“…1.58, δ 2.72 vs. 2.00 and δ 1.07 vs. 0.96, respectively), which could be ascribed to a deshielding effect of the hydroxyl group on H-8, Hax-11, and H-18. 93 This compound (i.e., STS 433) was previously described as a product of DNG metabolism by the nitrile hydratase-containing microorganism Rhodococcus erythropolis. 93 Aromatic A was isolated at ~ 5% yield at pH 7, and formed at trace levels at pH 5. hereafter referred to as "Aromatic B", all agree with those of known DNG metabolite STS 825, 93,[96][97] which has also been identified as a product of DNG photolysis in acetonitrile.…”

“…93 This compound (i.e., STS 433) was previously described as a product of DNG metabolism by the nitrile hydratase-containing microorganism Rhodococcus erythropolis. 93 Aromatic A was isolated at ~ 5% yield at pH 7, and formed at trace levels at pH 5. hereafter referred to as "Aromatic B", all agree with those of known DNG metabolite STS 825, 93,[96][97] which has also been identified as a product of DNG photolysis in acetonitrile. 98 Although the photolysis of DNG has previously been examined, its irradiation in organic solvent, which lead to some variation in product distribution relative to the results reported herein, is not environmentally relevant.…”