Nitro-<i>L</i>-Arginine Interferes with the Cadmium Reduction of Nitrate/<i>Griess </i>Reaction Method of Measuring Nitric Oxide Production

Nithipatikom, Kasem; Pratt, Phillip F.; Campbell, William B.

doi:10.1515/cclm.1996.34.2.133

Cited by 8 publications

(10 citation statements)

References 14 publications

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“…Absorbance was converted to concentration using a calibration curve prepared with sodium nitrite in distilled water. Serum nitrate was measured as nitrite after enzymatic reduction by an improved method (26)(27)(28). Values obtained by this procedure represent the sum of nitrite and nitrate.…”

Section: Serum Nitrite Levels (Nox)mentioning

confidence: 99%

Ghrelin, Nitrite and Paraoxonase/Arylesterase Concentrations in Cement Plant Workers

Aydın¹,

Aydın²,

Croteau³

et al. 2010

Journal of Medical Biochemistry

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Kratak sadr`aj: Izlaganje cementnoj pra{ini na radnom mestu povezano je s pove}anim rizikom od promena na jetri, plu}nih bolesti i nastanka kancera. Mogu}i mehanizmi koji uzrokuju bolesti su smanjeni antioksidativni kapacitet i po vi{e na lipidna peroksidacija u plazmi. U skladu s tim, u ovoj studiji prou~avani su nivoi paraoksonaze (PON1) -arilesteraze (AE), grelina, HDL-C, LDL-C i nitrita (NOx) u serumu kod radnika izlo`enih cementnoj pra{ini. U studiji je u~e stvovalo dvadeset osam radnika u cementari i 30 dobro voljaca (kontrola), starosti 29-54 godine. Koncentra cije PON1, AE, NOx, grelina i HDL-holesterola i LDL-holesterola u serumu merene su u obe grupe. PON-1, AE, grelin i HDL-holesterol bili su ni`i kod radnika u cementari nego u kontrolnoj grupi. Nivoi nitrita i LDL-C u serumu kod radnika u cementari bili su vi{i (p<0,05) nego u kontrolnoj grupi radnika. Nije prime}ena korelacija izme|u nivoa HDL-holesterola i PON1 u serumu, kao ni izme|u HDL-holesterola i grelina. Slaba nega tivna korelacija otkrivena je izme|u grelina i NOx u serumu. Rezultati studije sna`no ukazuju na to da HDL-paraoksonaza, AE, grelin, HDL-C i visoki nivoi NOx i LDL-C verovatno igraju ulogu u bolesti koja uklju~uje oksidativna o{te}enja. Me|utim, potrebna su dalja istra`ivanja kako bi se ispitala veza izme|u izlaganja pra{ini na radnom mestu i respiratornih simptoma.

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Section: Serum Nitrite Levels (Nox)mentioning

confidence: 99%

Ghrelin, Nitrite and Paraoxonase/Arylesterase Concentrations in Cement Plant Workers

Aydın¹,

Aydın²,

Croteau³

et al. 2010

Journal of Medical Biochemistry

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“…These compounds include ascorbate, reduced thiols, phosphate, N G -nitro-L-arginine and heparin [28,[33][34][35][36][37]. Most of these compounds can cause interference in both enzyme-reduction and cadmiumreduction methods.…”

Section: Sample Preparation and Other Considerationsmentioning

confidence: 99%

Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay

Sun

Zhang

Broderick

et al. 2003

Sensors

592

301

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“…This method is based on the principle of measuring the absorbance at 545 nm of the complex formed when nitrate is reduced to nitrite in the presence of nitrate reductase enzyme, and the resulting nitrite reacts with sulfanylamide and N-ethylendiamin. 31 …”

Section: Methodsmentioning

confidence: 99%

The effect of iloprost and sildenafil, alone and in combination, on myocardial ischaemia and nitric oxide and irisin levels

Aydın¹,

Kuloğlu²,

Aydın³

et al. 2017

CVJA

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SummaryAimInsufficient oxygen supply to organs and tissues due to reduced arterial or venous blood flow results in ischaemia, during which, although ATP production stops, AMP and adenosine continue to be produced from ATP. The fate of irisin, which causes the production of heat instead of ATP during ischaemia, is unknown. Iloprost and sildenafil are two pharmaceutical agents that mediate the resumption of reperfusion (blood supply) via vasodilatation during ischaemic conditions. Our study aimed to explore the effects of iloprost and sildenafil on irisin levels in the heart, liver and kidney tissues and whether these pharmaceutical agents had any impact on serum irisin and nitric oxide levels in rats with induced experimental myocardial ischaemia.MethodsThe study included adult male Sprague-Dawley rats aged 10 months and weighing between 250 and 280 g. The animals were randomly allocated to eight groups, with five rats in each group. The groups were: sham (control), iloprost (ILO), sildenafil (SIL), ILO + SIL, myocardial ischaemia (MI), MI + ILO, MI + SIL and MI + ILO + SIL. The treatment protocols were implemented before inducing ischaemia, which was done by occluding the left coronary artery with a plastic ligature for 30 minutes. Following the reperfusion procedure, all rats were sacrificed after 24 hours, and their heart, liver and kidney tissues and blood samples were collected for analyses. An immunohistochemical method was used to measure the change in irisin levels, the ELISA method to quantify blood irisin levels, and Griess’ assay to determine nitric oxide (NO) levels in the serum and tissue. Myocardial ischaemia was confirmed based on the results of Masson’s trichrome staining, as well as levels of troponin and creatine kinase MB.ResultsIrisin levels in biological tissue and serum dropped statistically significantly in the ischaemic group (MI), but were restored with ILO and SIL administration. Individual SIL administration was more potently restorative than individual ILO administration or the combined administration of the two agents. NO level, on the other hand, showed the opposite tendency, reaching the highest level in the MI group, and falling with the use of pharmaceutical agents.ConclusionsIndividual or combined administration of ILO and SIL reduced myocardial ischaemia and NO levels, and increased irisin levels. Elevated levels of irisin obtained by drug administration could possibly contribute to accelerated wound recovery by local heat production. Sildenafil was more effective than iloprost in eliminating ischaemia and may be the first choice in offsetting the effects of ischaemia in the future.

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Nitro-L-Arginine Interferes with the Cadmium Reduction of Nitrate/Griess Reaction Method of Measuring Nitric Oxide Production

Cited by 8 publications

References 14 publications

Ghrelin, Nitrite and Paraoxonase/Arylesterase Concentrations in Cement Plant Workers

Ghrelin, Nitrite and Paraoxonase/Arylesterase Concentrations in Cement Plant Workers

Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay

The effect of iloprost and sildenafil, alone and in combination, on myocardial ischaemia and nitric oxide and irisin levels

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