Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

He, Xiang; Zhang, Hao; Ye, Xiangsheng; Hong, Juan; Ding, Guangda

doi:10.3390/plants10102160

Cited by 11 publications

(7 citation statements)

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“…4), which is a known N-responsive element in other plants (Lea et al 2007;Konishi and Yanagisawa 2010). In addition, presence of MYC, G-Box, GATA-motif is also reported to be N-responsive in different plants (Witko 2003;Konishi and Yanagisawa 2013;Zhang et al 2015;He et al 2021;Islam et al 2021). Though there is no report on N-responsive elements in wheat genes, this analysis confirms N-responsive of the above elements in wheat.…”

Section: Discussionsupporting

confidence: 62%

“…However, the list of these N-responsive elements in NR gene may not be complete and comprehensive, as there are still gaps in the reference genome sequence. Presence of these N-responsive elements are occurring in combination with the phytohormoneresponsive elements in the promoter region indicates the complex regulatory network between the N assimilation and hormonal pathways (He et al 2021).…”

Section: Discussionmentioning

confidence: 99%

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Characterization and Expression Analysis ofNitrate Reductase 6-1ABDGene in Hexaploid Bread Wheat Under Different Nitrogen Regime

Gayatri

Ravi

Chauhan

et al. 2023

Preprint

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Nitrate reductase (NR) is the key rate-limiting enzyme of the nitrogen (N) assimilation process in plants, which has not been characterized in bread wheat under nitrogen stress, especially with respect to their homeologues. Total 9 NRs were identified and classified into 3 groups, which showed a close relationship with different wheat ancestors. The occurrence of N-responsive cis-acting regulatory elements like MYB, MYC, G-Box and GATA-motif confirmed their N-responsiveness. Expression of all the three groups of NR under N-stress revealed NR 6-1ABD group to be the most N-responsive, which was characterized further in detail. The study was carried out in two genotypes contrasting for their N-responsiveness (HD 2967: Highly responsive to applied N, and Choti Lerma: Less responsive to applied N) selected on the basis of field evaluation. Homeologous differences within a genotype were found much more than the genotypic differences of a specific homeologue coding sequence. Among the three homeologues, though NR 6-1D homeologue was found most responsive to N-stress, the contribution was maximum for this homeologue followed by NR 6-1A and least by NR 6-1B. We found that the expression of homeologues was linked to the presence of N-responsive cis-elements. All the homeologues of NR 6-1 in Choti Lerma were found less responsive to N-stress, in comparison to HD 2967, which might also be linked to N-use efficiency. Homeologous expression of NR 6-1ABD revealed negligible contribution of B-homeologue to N-stress. Homeologous differences of NR 6-1ABD was found much more than the genotypic differences. Hence, our study on wheat NR will be helpful in manipulating the specific homeologue of the NR gene in the future.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Characterization and Expression Analysis ofNitrate Reductase 6-1ABDGene in Hexaploid Bread Wheat Under Different Nitrogen Regime

Gayatri

Ravi

Chauhan

et al. 2023

Preprint

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“…According to KEGG pathway enrichment analysis, the DEGs associated with drought stress were assigned to phenylalanine biosynthesis ( ANTHOCYANIDIN REDUCTASE ) at the beginning of the phenylalanine metabolic pathway, in which phenylalanine ammonia lyase (PAL) acts not only as the rate‐limiting enzyme, but also as the key enzyme in response to drought, salt, and cold stress (Savoi et al, 2016). Studies show that amino acid metabolism in plants, as the precursor of many metabolites, has multiple functions in plant growth and stress responses (Ding, 2021; Thomas et al, 2008). In terms of tolerance to drought stress (Mostafa & Wang, 2020; Zhang et al, 2022c, d), nitrogen metabolism plays a non‐negligible role in resistance to drought stress.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Met is a precursor for the synthesis of ethylene, polyamines, and glucosinolates, and Met is converted to glycine to regulate seed development. Additionally, metabolites improve the ability of plants to resist abiotic stress (Di et al, 2002; Ding, 2021; Lu et al, 2011; Thomas et al, 2008). At the same time, it was found that the amino acid mixture improved the morphology and biochemical parameters, reduced the salt damage and increased the yield of broad bean (Abdelhamid et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Response of exogenous melatonin on transcription and metabolism of soybean under drought stress

Cao,

Zou,

Qin

et al. 2023

Physiologia Plantarum

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Amino acid metabolism is an important factor in regulating nitrogen source assimilation and source/sink transport in soybean. Melatonin can improve plant stress resistance, but whether it affects amino acid metabolism is not known. Therefore, this study investigated whether exogenous melatonin had an effect on amino acid metabolism of soybean under drought conditions and explored its relationship with yield. The treatments were normal water supply treatment (WW), drought stress treatment (D), drought stress and melatonin treatment group (D + M), sprayed with 100 μmol/L melatonin. The effects of melatonin on amino acid metabolism and grain filling were studied by physiological and omics experiments using Kangxian 9 (drought‐sensitive variety) and Suinong 26 (drought‐resistant variety) soybean cultivars. The results showed that drought stress decreased the activity of carbon and nitrogen metabolizing enzymes, which inhibited the accumulation of dry matter and protein, and decreased the yield. In the drought‐sensitive soybean variety, glycoenzymes and amino acid synthetases synthetic genes were upregulated in melatonin‐treated soybeans, hence carbon and nitrogen metabolism enzyme activity increased, increasing the carbohydrate and amino acid contents simultaneously. This resulted in higher dry matter and yield than drought‐stressed soybean not treated with melatonin. In the drought‐resistant variety, the grain weight per plant increased by 7.98% and 6.57% in 2020 and 2021, respectively, while it increased by 23.20% and 14.07% in the drought‐sensitive variety during the respective years. In conclusion, melatonin treatment can enhance the activity of nitrogen and carbon metabolism and amino acid content by upregulating the expression of soybean metabolic pathway and related genes, thus increasing the yield of soybean under drought stress.

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“…The GS , GOGAT , and NR gene families have been studied in other plant species, like rice, pecan, and rapeseed ( He et al, 2021 ; Li et al, 2022 ; Qiao et al, 2023 ), a comprehensive genome-wide analysis of these gene families in soybean is still lacking. This study aims to fill this gap by identifying and analyzing these gene families in six legume species: Glycine max , Cicer arietinum , Lotus japonicus , Phaseolus lunatus , Phaseolus vulgaris , and Vigna unguiculata , with chromosome numbers of 20, 8, 6, 11, 11, and 11, respectively.…”

Section: Introductionmentioning

confidence: 99%

Genomic organization and expression profiles of nitrogen assimilation genes in Glycine max

Elsanosi,

Zhu,

Zhou

et al. 2024

PeerJ

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Background Glutamine synthetase (GS), glutamate synthase (GOGAT), and nitrate reductase (NR) are key enzymes involved in nitrogen assimilation and metabolism in plants. However, the systematic analysis of these gene families lacked reports in soybean (Glycine max (L.) Merr.), one of the most important crops worldwide. Methods In this study, we performed genome-wide identification and characterization of GS, GOGAT, and NR genes in soybean under abiotic and nitrogen stress conditions. Results We identified a total of 10 GS genes, six GOGAT genes, and four NR genes in the soybean genome. Phylogenetic analysis revealed the presence of multiple isoforms for each gene family, indicating their functional diversification. The distribution of these genes on soybean chromosomes was uneven, with segmental duplication events contributing to their expansion. Within the nitrogen assimilation genes (NAGs) group, there was uniformity in the exon-intron structure and the presence of conserved motifs in NAGs. Furthermore, analysis of cis-elements in NAG promoters indicated complex regulation of their expression. RT-qPCR analysis of seven soybean NAGs under various abiotic stresses, including nitrogen deficiency, drought-nitrogen, and salinity, revealed distinct regulatory patterns. Most NAGs exhibited up-regulation under nitrogen stress, while diverse expression patterns were observed under salt and drought-nitrogen stress, indicating their crucial role in nitrogen assimilation and abiotic stress tolerance. These findings offer valuable insights into the genomic organization and expression profiles of GS, GOGAT, and NR genes in soybean under nitrogen and abiotic stress conditions. The results have potential applications in the development of stress-resistant soybean varieties through genetic engineering and breeding.

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Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses

Cited by 11 publications

References 70 publications

Characterization and Expression Analysis ofNitrate Reductase 6-1ABDGene in Hexaploid Bread Wheat Under Different Nitrogen Regime

Characterization and Expression Analysis ofNitrate Reductase 6-1ABDGene in Hexaploid Bread Wheat Under Different Nitrogen Regime

Response of exogenous melatonin on transcription and metabolism of soybean under drought stress

Genomic organization and expression profiles of nitrogen assimilation genes in Glycine max

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