Carbon Nanosphere (CNs) has been successfully synthesized from bamboo fibers at low temperatures by carbonization and activation. For activation used Potassium Hydroxide (KOH) at temperature 105°C, 155°C, 205°C, 255°C and 305°C. X-Ray Diffraction (XRD) spectra shows hexagonal and amorphous phase and Fourier Transform Infrared (FTIR) spectra shows decrease C-O bond with increasing activation temperature. Transmission Electron Microscopy (TEM) image for activation temperature of 105°C confirmed that sources the formation of Carbon Nanosphere. In this study shows bamboo fiber has a high potential as a carbon nanosphere material.