Nitrogen requirements for maximizing petroleum bioremediation in a sub-Antarctic soil

Walworth, James; Pond, Andrew; Snape, Ian; Rayner, J. C. W.; Ferguson, Susan H.; Harvey, Paul

doi:10.1016/j.coldregions.2006.07.001

Cited by 131 publications

(101 citation statements)

References 18 publications

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“…However, it is rarely necessary to add the total amount of N and P called for by this theoretical ratio because nutrients are recycled during the course of treatment as microorganisms turnover, especially in the case of oily sludge which has high concentrations of C. It has been demonstrated that excess nitrogen can depress the rate of microbial activity and petroleum degradation in contaminated soils due to depression of osmotic soil water potential [20]. In this study, manure addition significantly increased the counts of TPH and PAH degraders in the sludge compared with the control ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Prepared bed bioremediation of oily sludge in an oilfield in northern China

Liu

Luo

Teng

et al. 2009

Journal of Hazardous Materials

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a b s t r a c tField-scale bioremediation of oily sludge in prepared beds was studied at Shengli oilfield in northern China. The influence of manure, coarse sand, sawdust, a specialized microbial preparation and greenhouse conditions on the efficiency of removal of oil and grease was evaluated. After bioremediation for 230 d, oil and grease content fell by 32-42 g kg −1 dry sludge in treated plots, indicating removal of 27-46% compared with only 15% in the control plot. Addition of manure, coarse sand, sawdust and greenhouse conditions significantly (p < 0.05) increased the amount removed. Moreover, the physico-chemical properties of the sludge in all treated plots improved significantly after bioremediation. Microbial biomass in sludge and community-level physiological profiling examined using BIOLOG microplates was also studied. Total petroleum hydrocarbon degraders and polycyclic aromatic hydrocarbon degraders increased in all treated oily sludge. The activity of sludge microbial communities increased markedly in the treated plots compared with the control. Canonical correspondence analysis showed that differences in substrate utilization patterns were highly correlated (p < 0.05) with sludge hydrolyzable N and oil and grease content. The biological toxicity of the oily sludge was lower following bioremediation in most of the treated plots as evaluated using Photobacterium phosphoreum T3.

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Section: Discussionmentioning

confidence: 99%

Prepared bed bioremediation of oily sludge in an oilfield in northern China

Liu

Luo

Teng

et al. 2009

Journal of Hazardous Materials

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“…Several options for remediation were assessed, and the limitations of natural hydrocarbon degradation were investigated [15]. Based on a series of laboratory studies, in situ remediation, which enhanced microbial hydrocarbon degradation by aeration and nutrient addition, was determined to be the best option [16]. Full-scale onsite remediation began in 2009.…”

Section: Field Sitementioning

confidence: 99%

“…In cold regions, these natural processes take extended periods of time, but can be enhanced through remediation practices. For example, adding nutrients and oxygen stimulate microbial populations, enabling them to degrade hydrocarbons faster and encouraging further volatilization [15,16]. Testing the toxicity of aged and fresh petroleum hydrocarbons in soils allows for realistic measures of toxicity, because the contaminants interact with the environment and change through time.…”

Section: Introductionmentioning

confidence: 99%

Toxicity of diesel contaminated soils to the subantarctic earthworm Microscolex macquariensis

Mooney

King

Wasley

et al. 2012

Enviro Toxic and Chemistry

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Abstract-Several fuel spills have occurred on subantarctic Macquarie Island (54830 0 S 158857 0 E) associated with storing fuel and generating power for the island's research station. The Australian Antarctic Division began full-scale, on-site remediation of these sites in 2009. To develop appropriate target concentrations for remediation, acute and chronic tests were developed with the endemic earthworm, Microscolex macquariensis, using avoidance, survival, and reproduction as endpoints. Uncontaminated low (3%), medium (11%), and high (38-48%) carbon content soils from Macquarie Island were used to examine the influence of soil carbon on toxicity. Soils were spiked with Special Antarctic Blend (SAB) diesel and used either immediately to simulate a fresh spill or after four weeks to simulate an aged spill. Earthworms were sensitive to fresh SAB, with significant avoidance at 181 mg/kg; acute 14-d survival median lethal concentration (LC50) of 103 mg/kg for low carbon soil; and juvenile production median effective concentration (EC50) of 317 mg/kg for high carbon soil. Earthworms were less sensitive to aged SAB than to fresh SAB in high carbon soil for juvenile production (EC50 of 1,753 and 317 mg/kg, respectively), but were more sensitive for adult survival (LC50 of 2,322 and 1,364 mg/kg, respectively). Using M. macquariensis as a surrogate for soil quality, approximately 50 to 200 mg SAB/kg soil would be a sufficiently protective remediation target.

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“…The negative environmental impacts and high costs associated with excavating and transporting contaminated soils for off-site processing and treatment have led the Australian Antarctic Division (AAD) to abandon dig-and-haul methods in favour of implementing alternative onsite in situ remediation techniques [2,3]. The in situ techniques utilised on subantarctic Macquarie Island include injection of nutrients and aeration into the soils, both of which promote natural attenuation by encouraging hydrocarbon-degrading microbes [3,[10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…This study is focused on subantarctic Macquarie Island, for which there is data for biological responses to total petroleum hydrocarbons (TPH) exposure for earthworms [21], soil microbes [13,14] and the grass Poa foliosa [22]. This is insufficient information to enable guideline development to assess the risk of fuel-contaminated soils on native subantarctic species.…”

Section: Introductionmentioning

confidence: 99%

Impact of hydrocarbons from a diesel fuel on the germination and early growth of subantarctic plants

Macoustra

King

Wasley

et al. 2015

Environ. Sci.: Processes Impacts

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. (2015). Impact of hydrocarbons from a diesel fuel on the germination and early growth of subantarctic plants. Environmental Science Processes and Impacts, 17 (7), 1238-1248. Impact of hydrocarbons from a diesel fuel on the germination and early growth of subantarctic plants AbstractSpecial Antarctic Blend (SAB) is a diesel fuel dominated by aliphatic hydrocarbons that is commonly used in Antarctic and subantarctic regions. The past and present use of SAB fuel at Australia's scientific research stations has resulted in multiple spills, contaminating soils in these pristine areas. Despite this, no soil quality guidelines or remediation targets have been developed for the region, primarily due to the lack of established indigenous test species and subsequent biological effects data. In this study, twelve plant species native to subantarctic regions were collected from Macquarie Island and evaluated to determine their suitably for use in laboratory-based toxicity testing, using germination success and seedling growth (shoot and root length) as endpoints. Two soil types (low and high organic carbon (OC)) were investigated to reflect the variable OC content found in soils on Macquarie Island. These soils were spiked with SAB fuel and aged for 14 days to generate a concentration series of SAB-contaminated soils. Exposure doses were quantified as the concentration of total petroleum hydrocarbons (TPH, nC9-nC18) on a soil dry mass basis. Seven species successfully germinated on control soils under laboratory conditions, and four of these species (Colobanthus muscoides Hook.f., Deschampsia chapmanii Petrie, Epilobium pendunculare A.Cunn. and Luzula crinita Hook.f.) showed a dose-dependent inhibition of germination when exposed to SAB-contaminated soils. Contaminated soils with low OC were generally more toxic to plants than high organic carbon soils. Increasing soil-TPH concentrations significantly inhibited shoot and root growth, and root length was identified as the most sensitive endpoint. Although the test species were tolerant to SAB-contaminated soils in germination assays, development of early life stages (up to 28 days) were generally more sensitive indicator of exposure effects, and may be more useful endpoints for future testing. Disciplines Medicine and Health Sciences | Social and Behavioral Sciences Publication DetailsMacoustra, G. K., King, C. K., Wasley, J., Robinson, S. A. & Jolley, D. F. (2015). Impact of hydrocarbons from a diesel fuel on the germination and early growth of subantarctic plants. Environmental Science Processes and Impacts, 17 (7) Keywords: total petroleum hydrocarbons, total organic carbon, monocots, dicots, germination, root and shoot growth. 2 ABSTRACTSpecial Antarctic Blend (SAB) is a diesel fuel dominated by aliphatic hydrocarbons that is commonly used in Antarctic and subantarctic regions. The past and present use of SAB fuel at Australia's scientific research stations has resulted in multiple spills, contaminating soils in these pristine areas. Despite this, no soil quality guidel...

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Nitrogen requirements for maximizing petroleum bioremediation in a sub-Antarctic soil

Cited by 131 publications

References 18 publications

Prepared bed bioremediation of oily sludge in an oilfield in northern China

Prepared bed bioremediation of oily sludge in an oilfield in northern China

Toxicity of diesel contaminated soils to the subantarctic earthworm Microscolex macquariensis

Impact of hydrocarbons from a diesel fuel on the germination and early growth of subantarctic plants

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