Nitrogen uptake and remobilization in tetraploid 'Langdon' durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1

Kade, M.; Barneix, Atilio J.; Olmos, Sofı́a; Dubcovsky, Jorge

doi:10.1111/j.1439-0523.2005.01110.x

Cited by 79 publications

(70 citation statements)

References 27 publications

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“…RubisCO). This hypothesis is supported by previous studies in tetraploid wheat, which showed that lines with the non-functional GPC-B1 allele had significantly lower concentrations of soluble amino acids in the flag leaf than their corresponding isogenic lines carrying the functional GPC-B1 allele (Kade et al 2005). The concentration of soluble amino acids is important because a substantial percentage of the N in the wheat grain is supplied by amino acids remobilized from vegetative tissues (Barneix 2007;Gregersen et al 2008;Waters et al 2009).…”

Section: N Accumulation During Grain Developmentsupporting

confidence: 73%

“…Increasing the rate of N remobilization to developing grains utilizes stored N more efficiently and increases GPC (Kade et al 2005). While most of the carbohydrates found in the wheat grain are the result of assimilation during the grain filling period (Austin et al 1977a;Blake et al 2007), grain protein is predominantly derived from the remobilization of N from degraded leaf proteins, which are stored during wheat vegetative growth (Dalling et al 1976;Austin et al 1977b;Simpson et al 1983;Kichey et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Functional characterization of GPC-1 genes in hexaploid wheat

Avni

Zhao

Pearce

et al. 2013

Planta

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In wheat, monocarpic senescence is a tightly regulated process during which nitrogen (N) and micronutrients stored pre-anthesis are remobilized from vegetative tissues to the developing grains. Recently, a close connection between senescence and remobilization was shown through the map-based cloning of the GPC (Grain Protein Content) gene in wheat. GPC-B1 encodes a NAC transcription factor associated with earlier senescence and increased grain protein, iron and zinc content, and is deleted or non-functional in most commercial wheat varieties. In the current research, we identified 'loss of function' ethyl methane sulphonate mutants for the two GPC-B1 homoeologous genes; GPC-A1 and GPC-D1, in a hexaploid wheat mutant population. The single gpc-a1 and gpc-d1 mutants, the double gpc-1 mutant and control lines were grown under field conditions at four locations and were characterized for senescence, GPC, micronutrients and yield parameters. Our results show a significant delay in senescence in both the gpc-a1 and gpc-d1 single mutants and an even stronger effect in the gpc-1 double mutant in all the environments tested in this study. The accumulation of total N in the developing grains showed a similar increase in the control and gpc-1 plants until 25 days after anthesis (DAA) but at 41 and 60 DAA the control plants had higher Grain N content than the gpc-1 mutants. At maturity, GPC in all mutants was significantly lower than in control plants while grain weight was unaffected. These results demonstrate that theGPC-A1 and GPC-D1 genes have a redundant function and play a major role in the regulation of monocarpic senescence and nutrient remobilization in wheat.

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Section: N Accumulation During Grain Developmentsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Functional characterization of GPC-1 genes in hexaploid wheat

Avni

Zhao

Pearce

et al. 2013

Planta

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“…Uauy et al (2006a) discovered that the Gpc-B1 DIC allele accelerates leaf senescence and suggested that the differences in GPC were pleiotropic effects of the changes in senescence. The effect of Gpc-B1 on senescence also explains the higher levels of soluble proteins and amino acids observed in flag leaves after anthesis in plants carrying the DIC Gpc-B1 allele relative to those with the LDN allele (Kade et al 2005). Chromosome 6B from DIC has been previously associated with higher grain mineral concentrations (Cakmak et al 2004), an effect that was later determined to be associated with the 250 kb region including Gpc-B1 (Distelfeld et al 2007).…”

Section: Introductionmentioning

confidence: 94%

Colinearity between the barley grain protein content (GPC) QTL on chromosome arm 6HS and the wheat Gpc-B1 region

et al. 2008

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“…Even so, higher rates of N remobilization were associated with the higher grain protein concentration of nearly isogenic recombinant substitution lines carrying the high grain protein concentration Gpc-B1 locus from wild emmer wheat (Triticum turgidum subsp. dicoccoides; Kade et al, 2005;Uauy et al, 2006). All of these results indicate that efforts to increase N availability during grain filling should focus on processes related to N assimilation and temporary storage in vegetative organs rather than on protein degradation and translocation.…”

mentioning

confidence: 98%

Dynamics of Light and Nitrogen Distribution during Grain Filling within Wheat Canopy

2008

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In monocarpic species, during the reproductive stage the growing grains represent a strong sink for nitrogen (N) and trigger N remobilization from the vegetative organs, which decreases canopy photosynthesis and accelerates leaf senescence. The spatiotemporal distribution of N in a reproductive canopy has not been described in detail. Here, we investigated the role of the local light environment on the spatiotemporal distribution of leaf lamina N mass per unit leaf area (SLN) during grain filling of field-grown wheat (Triticum aestivum). In addition, in order to provide some insight into the coordination of N depletion between the different vegetative organs, N dynamics were studied for individual leaf laminae, leaf sheaths, internodes, and chaff of the top fertile culms. At the canopy scale, SLN distribution paralleled the light gradient below the flag leaf collar until almost the end of grain filling. On the contrary, the significant light gradient along the flag leaf lamina was not associated with a SLN gradient. Within the top fertile culms, the time course of total (alive 1 necrotic tissues) N concentration of the different laminae and sheaths displayed a similar pattern. Another common pattern was observed for internodes and chaff. During the period of no root N uptake, N depletion of individual laminae and sheaths followed a first-order kinetics independent of leaf age, genotype, or N nutrition. The results presented here show that during grain filling, N dynamics are integrated at the culm scale and strongly depend on the local light conditions determined by the canopy structure.

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Nitrogen uptake and remobilization in tetraploid 'Langdon' durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1

Cited by 79 publications

References 27 publications

Functional characterization of GPC-1 genes in hexaploid wheat

Functional characterization of GPC-1 genes in hexaploid wheat

Colinearity between the barley grain protein content (GPC) QTL on chromosome arm 6HS and the wheat Gpc-B1 region

Dynamics of Light and Nitrogen Distribution during Grain Filling within Wheat Canopy

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