NMDA receptor NR1 and NR2A/B subunit expression in trigeminal neurons during early postnatal development

Turman, Jack E.; Ajdari, Jurak; Chandler, Scott H.

doi:10.1002/(sici)1096-9861(19990628)409:2<237::aid-cne5>3.3.co;2-9

Cited by 5 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We recently reported that, during postnatal development, synapses onto JC motoneurons exhibit distinct time course of synaptogenesis, changes of synaptic strength, and phenotypic switch of inhibitory transmitters, in close relationship with the temporal pattern of maturation of the orofacial motor system (Paik et al,2007,2011). Similarly, distinct developmental changes were reported for AMPA, NMDA, and metabotropic glutamate receptors in Vmes neurons (Turman et al,1999,2000,2001). However, little is known about the development of morphologically and/or functionally defined synapses on the somata of Vmes neurons (Wu et al,2001; Lazarov,2002,2007; Saito et al,2006).…”

supporting

confidence: 55%

Identification of alpha‐actinin 4 and 67 kDa laminin receptor as stage‐specific markers in esophageal cancer via proteomic approaches

Qin

Xie

et al. 2007

Cancer

View full text Add to dashboard Cite

Unlike other primary sensory neurons, the neurons in the mesencephalic trigeminal nucleus (Vmes) receive most of their synaptic input onto their somata. Detailed description of the synaptic boutons onto Vmes neurons is crucial for understanding the synaptic input onto these neurons and their role in the motor control of masticatory muscles. For this, we investigated the distribution of γ‐aminobutyric acid (GABA)‐, glycine‐, and glutamate‐immunopositive (+) boutons on Vmes neurons and their ultrastructural parameters that relate to transmitter release: Vmes neurons that innervate masseteric muscle spindles were identified by labeling with horseradish peroxidase injected into the muscle, and immunogold staining and quantitative ultrastructural analysis of synapses onto these neurons were performed in adult rats and during postnatal development. The bouton volume, mitochondrial volume, and active zone area of the boutons contacting labeled somata (axosomatic synapses) were similar to those of boutons forming axoaxonic synapses with Vmes neurons but smaller than those of boutons forming axodendritic or axosomatic synapses with most other neurons. GABA+, glycine+, and glutamate+ boutons constituted a large majority (83%) of all boutons on labeled somata. A considerable fraction of boutons (28%) was glycine+, and all glycine+ boutons were also GABA+. Bouton size remained unchanged during postnatal development. These findings suggest that the excitability of Vmes neurons is determined to a great extent by GABA, glycine, and glutamate and that the relatively lower synaptic strength of axosomatic synapses may reflect the role of the Vmes neurons in modulating orofacial motor function. J. Comp. Neurol. 520:3414–3427, 2012. © 2012 Wiley Periodicals, Inc.

show abstract

supporting

confidence: 55%

Identification of alpha‐actinin 4 and 67 kDa laminin receptor as stage‐specific markers in esophageal cancer via proteomic approaches

Qin

Xie

et al. 2007

Cancer

View full text Add to dashboard Cite

show abstract

“…However, electrophysiological studies have shown that the duration of excitatory postsynaptic potentials declines severalfold during the first 3 postnatal weeks (Hestrin,1992; Burgard and Hablitz,1993; Sanes,1993). In addition, the expression of various glutamate receptors subunits by neurons in the trigeminal motor nucleus is also temporally and spatially regulated during development (Turman et al,1999,2000,2001,2002; Ishihama et al,2005; Ishihama and Turman,2006). This is consistent with the possibility that the change in the functional properties of glutamatergic transmission to the JC motoneurons during postnatal development is mediated by a change in the kinetics and subtypes of glutamate receptors expressed by these neurons, without the need for significant change in the fraction of Glut + boutons.…”

Section: Discussionmentioning

confidence: 99%

“…The activities of JC muscles increase during development (Ahlgren,1966; Inoue et al,1989), reflecting increased firing frequency of JC motoneurons (Chandler et al,1994; Inoue et al,1999; Hsiao et al,2002). The developmental changes in expression of various subtypes of glutamate receptors, including AMPA, NMDA, and metabotropic glutamate receptors by the postsynaptic motoneurons have been also studied in detail (Turman et al,1999,2000,2001,2002; Ishihama et al,2005; Ishihama and Turman,2006). In contrast, little information is available on the changes in the presynaptic element—the excitatory boutons onto JC motoneurons—during development.…”

mentioning

confidence: 99%

Liver function critically determines serum retinol-binding protein 4 (RBP4) levels in patients with chronic liver disease and cirrhosis

Tacke

Weiskirchen

2008

Hepatology

View full text Add to dashboard Cite

We read with great interest the recent work by Petta et al. suggesting serum levels of retinol-binding protein 4 (RBP4) as a marker for the degree of steatosis in patients infected with hepatitis C virus (HCV). 1 RBP4 has provoked exceptional attention because it has been linked to the pathogenesis of insulin resistance in mice and humans. 2,3 Petta et al. now reported an association between elevated serum RBP4 and hepatic steatosis as well as insulin resistance in patients with nonalcoholic steatohepatitis (NASH), and serum RBP4 appeared to be a strong independent predictor of hepatic steatosis in nondiabetic, nonobese patients chronically infected with HCV genotype 1. 1 However, from our own 4 and other studies, 5 we would like to stress important potential confounding factors that have neither been evaluated nor discussed by the authors and might greatly hamper the diagnostic usefulness of serum RBP4 in patients with liver disease in the real clinical setting. It is well known that the liver is the major source of circulating RBP4 in humans, 3 and therefore the hepatic biosynthetic capacity may greatly influence serum RBP4. Although Petta's study excluded patients with Child B and Child C cirrhosis, we could demonstrate in a large cohort of patients with chronic liver diseases of various origins 6,7 that serum RBP4 significantly decreased with the stage of liver cirrhosis (Fig. 1A). 4 Moreover, these differences remained also significant, if patients without cirrhosis were compared to healthy controls or to patients with Child A cirrhosis, which were the subgroups of patients included in the current study by Petta et al. Concordantly, serum RBP4 closely correlated with indicators of liver function in our analysis, such as cholinesterase (r ϭ 0.639), albumin (r ϭ 0.482), or coagulation factors II (r ϭ 0.641) or VII (r ϭ 0.647, all P Ͻ 0.001), in patients with chronic liver diseases. 4 We corroborated this finding in an animal model of liver fibrosis, as the hepatic RBP4 messenger RNA expression was significantly reduced in rats after surgical biliary obstruction compared to sham-operated controls. 4 Interestingly, these findings were confirmed in an independent study by Bahr et al., attributing reduced serum RBP4 in patients with liver cirrhosis to a decreased hepatic RBP4 synthesis. 5 We now performed an additional analysis, comparing patients with absent to mild (Յ30%) and moderate to severe (Ͼ30%) steatosis as determined by liver histology as suggested by Petta et al. Nonetheless, we could not confirm the reported increase in serum RBP4 concentrations in our patients (Fig. 1B). Instead, the degree of liver fibrosis and cirrhosis was the major histological parameter associated with reduced serum RBP4 (Fig. 1C). 4 Patients with viral hepatitis in our study did not significantly differ from other etiologies of liver disease (data not shown).We are well aware that the patient cohort of Petta's work considerably differed from our study, most likely accounting for the observed discrepancies. Whereas Petta...

show abstract

“…At present, the functional significance of these excitatory and inhibitory Vsup premotoneurons in jaw movement is unknown. Recently, it has been reported that various subtypes of glutamate receptors such as NMDA and groups I and II metabotropic glutamate receptors are expressed in the Vsup (Turman et al, 1999, 2001). We speculate that excitatory and inhibitory Vsup premotoneurons receive different types of peripheral inputs and are involved in neural circuits with different functions.…”

Section: Resultsmentioning

confidence: 99%

Ultrastructural analysis of glutamate‐, GABA‐, and glycine‐immunopositive boutons from supratrigeminal premotoneurons in the rat trigeminal motor nucleus

Paik¹,

Lee²,

Choi³

et al. 2008

J of Neuroscience Research

View full text Add to dashboard Cite

The supratrigeminal region (Vsup) is important for coordination of smooth jaw movement. However, little is known about the synaptic connections of the Vsup premotoneurons with the trigeminal motor neurons. In the present study, we examined axon terminals of Vsup premotoneurons in the contralateral trigeminal motor nucleus (Vmo) by a combination of anterograde tracing with cholera toxin B-horseradish peroxidase (CTB-HRP), postembedding immunohistochemistry for the amino acid transmitters glutamate, GABA, and glycine, and electron microscopy. Tracer injections resulted in anterograde labeling of axon terminals of the Vsup premotoneurons in the motor trigeminal nucleus (Vmo). The labeled boutons in Vmo exhibited immunoreactivity for glutamate, GABA, or glycine: glutamate-immunopositive boutons (69%) were more frequently observed than GABA- or glycine-immunopositive boutons (19% and 12%, respectively). Although most labeled boutons (97%) made synaptic contacts with a single postsynaptic dendrite, a few glutamate-immunopositive boutons (3%) showed synaptic contact with two dendrites. No labeled boutons participated in axoaxonic synaptic contacts. Most labeled boutons (78%) were presynaptic to dendritic shafts, and the remaining 22% were presynaptic to somata or primary dendrites. A large proportion of GABA- or glycine-immunopositive boutons (40%) were presynaptic to somata or primary dendrites, whereas most glutamate-immunopositive boutons (86%) were presynaptic to dendritic shafts. These results indicate that axon terminals of Vsup premotoneurons show simple synaptic connection with Vmo neurons. This may provide the anatomical basis for the neural information processing responsible for jaw movement control.

show abstract

NMDA receptor NR1 and NR2A/B subunit expression in trigeminal neurons during early postnatal development

Cited by 5 publications

References 0 publications

Identification of alpha‐actinin 4 and 67 kDa laminin receptor as stage‐specific markers in esophageal cancer via proteomic approaches

Identification of alpha‐actinin 4 and 67 kDa laminin receptor as stage‐specific markers in esophageal cancer via proteomic approaches

Liver function critically determines serum retinol-binding protein 4 (RBP4) levels in patients with chronic liver disease and cirrhosis

Ultrastructural analysis of glutamate‐, GABA‐, and glycine‐immunopositive boutons from supratrigeminal premotoneurons in the rat trigeminal motor nucleus

Contact Info

Product

Resources

About