2018
DOI: 10.1371/journal.pone.0200658
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NMR-based metabolic profiling of urine, serum, fecal, and pancreatic tissue samples from the Ptf1a-Cre; LSL-KrasG12D transgenic mouse model of pancreatic cancer

Abstract: Pancreatic cancer is the third leading cause of cancer deaths in the United States with more than 53,000 expected to be diagnosed with the disease in 2018. The median survival time after diagnosis is four to six months. The poor survival statistics are due in part to the fact that pancreatic cancer is typically asymptomatic until it reaches advanced stages of the disease. Although surgical resection provides the best chance of survival, pancreatic cancer is rarely detected when surgery is still possible due, i… Show more

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Cited by 11 publications
(13 citation statements)
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“…To be considered significant, a bucket had to have either a p -value <0.05 or an AUC >0.70 [58]. In MiaPaCa-2 cells, 53 (Table S1) metabolites out of a total of 74 metabolites identified (Table S2) were present at significantly different abundances depending on drying technique.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To be considered significant, a bucket had to have either a p -value <0.05 or an AUC >0.70 [58]. In MiaPaCa-2 cells, 53 (Table S1) metabolites out of a total of 74 metabolites identified (Table S2) were present at significantly different abundances depending on drying technique.…”
Section: Resultsmentioning
confidence: 99%
“…One-dimensional 1 H Carr-Purcell-Meiboom-Gill (CPMG) NMR spectra were collected using a spectral width of 20.0 ppm and 32K points resulting in 1.21 s acquisition time, 512 scans, 4 dummy scans, 1.80 s recycle delay and 1 ms mixing time. Two-dimensional 1 H- 1 H TOCSY NMR experiments were performed using a spectral width of 10.0 ppm and 2K points, 1.5 s recycle time and 60 ms mixing time [14,17,19,20,21,58,59,60,61]. Two-dimensional 1 H- 13 C HSQC NMR experiments were performed using non-uniform sampling in the indirect carbon dimension [62,63].…”
Section: Methodsmentioning
confidence: 99%
“…In addition, the association between linoleic acid metabolism and the severity of AP has been demonstrated [ 32 ]. Pentose and glucuronate interconversions are an insulin-relevant tissue-specific signaling pathway [ 33 ], which is the pathway associated with metabolites in fecal samples of patients with pancreatic cancer [ 34 ]. The pathological changes observed in the pancreatic tissues of the AP group were in line with the injuries induced by cerulein.…”
Section: Discussionmentioning
confidence: 99%
“…D16-00100). Surgical procedures were carried out as previously described 22–27. All authors completed online and in-class animal training prior to animal work.…”
Section: Methodsmentioning
confidence: 99%
“…All dissections and intestinal histologies were performed as described previously 22–29. Details regarding the histological procedures can be found in online supplementary material.…”
Section: Methodsmentioning
confidence: 99%