NMR-Based Metabolomics Revealed the Underlying Inflammatory Pathology in Reactive Arthritis Synovial Joints

Dubey, Durgesh; Kumar, Sandeep; Rawat, Atul; Guleria, Anupam; Kumari, Reena; Ahmed, Sakir; Singh, Rajeev; Misra, Ramnath; Kumar, Dinesh

doi:10.1021/acs.jproteome.1c00620

Cited by 4 publications

(9 citation statements)

References 88 publications

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“…Conversely, elevated levels of glucose in serum were observed in ReA patients (Guleria et al, 2019). Regarding lactate, the product of anaerobic glycolysis, PsA patients showed higher levels than those observed in RA patients, while two studies showed opposite results in ReA patients (Guleria et al, 2019;Souto-Carneiro et al, 2020;Dubey et al, 2021).…”

Section: Spondyloarthritis Cases Vs Rheumatoid Arthritis Casesmentioning

confidence: 97%

“…5 studies included JIA patients (Zeft et al, 2014;Stoll et al, 2016;Butbul et al, 2020;Vernocchi et al, 2020;Funk and Becker, 2021). 5 studies included ReA patients (Ahmed et al, 2019;Guleria et al, 2019;Li et al, 2019;Muhammed et al, 2020;Dubey et al, 2021). 2 studies included uSpA patients (Ahmed et al, 2019;Muhammed et al, 2020), 1 study included ERA patients (Stoll et al, 2016), 1 study included IBD-SpA (Berlinberg et al, 2021).…”

Section: Study Selection and Characteristicsmentioning

confidence: 99%

“…Nineteen studies assessed the metabolome by serum/plasma. Feces (Shao et al, 2016;Stoll et al, 2016;He et al, 2019;Vernocchi et al, 2020), urine (Kapoor et al, 2013;Nanus et al, 2015;Wang et al, 2016;Butbul et al, 2020), and synovial fluid (Ahmed et al, 2019;Li et al, 2019;Muhammed et al, 2020;Dubey et al, 2021) were commonly studied in these metabolomic researches. In addition, tissues such as ligament tissue (Wang et al, 2016), colon biopsies (Berlinberg et al, 2021), synovial membrane tissue (Rocha et al, 2021), saliva (Lv et al, 2021), and exhaled breath (Zeft et al, 2014) were also used for analysis in 1 study each.…”

Section: Study Selection and Characteristicsmentioning

confidence: 99%

“…Lipid profiles could be used to differentiate between PsA and RA to a large extent. Higher glycerol and cholesterol levels were found in both PsA and ReA patients than in RA patients (Madsen et al, 2010;Dubey et al, 2021). Additionally, ReA patients showed lower levels of LDL and VLDL in serum and synovial fluid (Guleria et al, 2019;Dubey et al, 2021).…”

Section: Spondyloarthritis Cases Vs Rheumatoid Arthritis Casesmentioning

confidence: 99%

“…Higher glycerol and cholesterol levels were found in both PsA and ReA patients than in RA patients (Madsen et al, 2010;Dubey et al, 2021). Additionally, ReA patients showed lower levels of LDL and VLDL in serum and synovial fluid (Guleria et al, 2019;Dubey et al, 2021). The level of acetate, however, was highly heterogeneous among different studies.…”

Section: Spondyloarthritis Cases Vs Rheumatoid Arthritis Casesmentioning

confidence: 99%

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Metabolomic analysis in spondyloarthritis: A systematic review

Huang

Pu²,

Wang³

et al. 2022

Front. Microbiol.

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Spondyloarthritis (SpA) is a group of rheumatic diseases that cause joint inflammation. Accumulating studies have focused on the metabolomic profiling of SpA in recent years. We conducted a systematic review to provide a collective summary of previous findings on metabolomic profiling associated with SpA. We systematically searched PubMed, Medline, Embase and Web of Science for studies on comparisons of the metabolomic analysis of SpA patients and non-SpA controls. The Newcastle–Ottawa Scale (NOS) was used to assess the quality of the included articles. From 482 records identified, 31 studies were included in the analysis. A number of metabolites were differentially distributed between SpA and non-SpA cases. SpA patients showed higher levels of glucose, succinic acid, malic acid and lactate in carbohydrate metabolism, higher glycerol levels and lower fatty acid (especially unsaturated fatty acid) levels in lipid metabolism, and lower levels of tryptophan and glutamine in amino acid metabolism than healthy controls. Both conventional and biological therapy of SpA can insufficiently reverse the aberrant metabolism state toward that of the controls. However, the differences in the results of metabolic profiling between patients with SpA and other inflammatory diseases as well as among patients with several subtypes of SpA are inconsistent across studies. Studies on metabolomics have provided insights into etiological factors and biomarkers for SpA. Supplementation with the metabolites that exhibit decreased levels, such as short-chain fatty acids (SCFAs), has good treatment prospects for modulating immunity. Further studies are needed to elucidate the role of disordered metabolic molecules in the pathogenesis of SpA.

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Section: Spondyloarthritis Cases Vs Rheumatoid Arthritis Casesmentioning

confidence: 97%

Section: Study Selection and Characteristicsmentioning

confidence: 99%

Section: Study Selection and Characteristicsmentioning

confidence: 99%

Section: Spondyloarthritis Cases Vs Rheumatoid Arthritis Casesmentioning

confidence: 99%

Section: Spondyloarthritis Cases Vs Rheumatoid Arthritis Casesmentioning

confidence: 99%

See 3 more Smart Citations

Metabolomic analysis in spondyloarthritis: A systematic review

Huang

Pu²,

Wang³

et al. 2022

Front. Microbiol.

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Clinical metabolomics by NMR revealed serum metabolic signatures for differentiating sarcoidosis from tuberculosis

Rai,

Yadav,

Jain

et al. 2023

Metabolomics

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Metabolomics-based Approach to Analyze the Therapeutic Targets and Metabolites of a Synovitis Ointment for Knee Osteoarthritis

Zhang

Yang

et al. 2023

CPA

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Background: Knee osteoarthritis (KOA) is a clinically common degenerative joint disease that is not fatal but has a high prevalence. Synovitis ointment (HMYG) is a traditional Chinese medicine formula that has been clinically proven to treat KOA; however, its therapeutic targets remain unknown. Objective: This study aimed to identify metabolites and potential targets of synovitis ointment alleviation in rats with KOA using ultra-high-performance liquid-chromatography–mass spectrometry (UHPLC-MS) metabolomics. Methods: The meniscus on each side of the knee was removed to model KOA in rats. The synovitis ointment treatment was provided for 4 weeks. The lateral diameter of the knee was measured once a week, and after 4 weeks, serum was collected to observe changes in the knee through a metabolomic analysis. Results: Synovitis ointment reduced the lateral diameter of the knee joint, relieved knee swelling, and improved knee volume. In total, 28 differential metabolites, which were mainly involved in arginine and proline metabolism and apoptosis, were identified in the Con and HMYG groups. 15-Deoxy-d-12,14-PGJ2 and fomepizole were found to be the key metabolites after the HMYG treatment of KOA. Compared with known drugs (diclofenac diethylamine emulsion and Jin Huang San), 2-(S-Glutathionyl) acetyl glutathione, daidzein, pelargonic acid, and sulfamethoxazole increased in the HMYG, and the metabolic pathways included the oxytocin signaling pathway, platelet activation, olfactory transduction, phototransduction, and cGMP–PKG signaling pathway. The expression levels of cleaved-caspase-3, Bcl-2, PIK3a, TP53, TGFB1, and NFKB1 were reversed after HMYG treatment. Conclusion: It has been observed that synovitis ointment relieves KOA. UHPLC–MS can analyze the potential mechanism of action of the herbal compound of the synovitis ointment.

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NMR-Based Metabolomics Revealed the Underlying Inflammatory Pathology in Reactive Arthritis Synovial Joints

Cited by 4 publications

References 88 publications

Metabolomic analysis in spondyloarthritis: A systematic review

Metabolomic analysis in spondyloarthritis: A systematic review

Clinical metabolomics by NMR revealed serum metabolic signatures for differentiating sarcoidosis from tuberculosis

Metabolomics-based Approach to Analyze the Therapeutic Targets and Metabolites of a Synovitis Ointment for Knee Osteoarthritis

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