NMR-based metabolomics reveals urinary metabolome modifications in female Sprague–Dawley rats by cranberry procyanidins

Liu, Haiyan; Tayyari, Fariba; Edison, Arthur S.; Su, Zhihua; Gu, Liwei

doi:10.1016/j.jnutbio.2016.05.007

Cited by 23 publications

(20 citation statements)

References 35 publications

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“…This report is the first identification of 3,3-HPHPA in the mouse (Table 1). Of 4 groups that analyzed rat urine for the presence of 3,3-HPHPA, 2 confirmed its presence [24,63] and 2 did not [32,38]. One group reported that inoculation of germ-free rats with feces from specific-pathogen-free rats had no effect on urinary 3,3-HPHPA [24].…”

Section: Gmb and The Small Phenolic Moleculesmentioning

confidence: 99%

Identification and Quantification by Targeted Metabolomics of Antibiotic-Responsive Urinary Small Phenolic Molecules Derived from the Intestinal Microbiota in Mice

Obrenovich

Jaskiw

Zhang

et al. 2019

PAI

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Background: Urinary levels of small molecules generated by the gut microbiota (GMB) constitute potential biomarkers for the state of the GMB. Such metabolites include numerous small phenolic molecules linked to anaerobic bacteria, particularly Clostridium species. Due to multiple technical challenges, however, the relationship between these chemicals and the GMB remains poorly characterized. Improved, high-performance liquid chromatography-mass spectrometry (LC-MS)-based metabolomic analysis can now reliably separate and quantify low levels of multiple small phenolic molecules and their structural isomers.Methods: CF-1 (female mice) were treated over 2 consecutive days with either i) vehicle, ii) one of 2 different antibiotic regimens (clindamycin or piperacillin/tazobactam) known to inhibit intestinal anaerobes and promote colonization by Clostridium difficile and other pathogens or iii) an antibiotic (aztreonam) that suppresses facultative Gram-negative bacteria but not enterococci or anaerobes and does not promote pathogen colonization Urine collected 24 hours after the last treatment was analyzed by LC-MS.Results: We identified over 25 compounds, many of which had not been previously reported in mouse urine. Eleven small phenolic molecules showed significant antibiotic-related changes. Urinary levels of the hydroxyphenylpropionic acids were suppressed by clindamycin and piperacillin/tazobactam treatment, but were elevated in aztreonam-treated mice. In addition, aztreonam treatment was associated with elevated levels of the dihydroxyhydrocinnamic acids.Conclusions: Profiles of differential changes in urinary small phenolic molecules may provide an index of anaerobic bacterial species in the GMB and could prove useful in monitoring susceptibility to overgrowth of pathogens such as C. difficile.

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Section: Gmb and The Small Phenolic Moleculesmentioning

confidence: 99%

Identification and Quantification by Targeted Metabolomics of Antibiotic-Responsive Urinary Small Phenolic Molecules Derived from the Intestinal Microbiota in Mice

Obrenovich

Jaskiw

Zhang

et al. 2019

PAI

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“…Several metabolomics studies have been published in attempts to identify biomarkers of cranberry consumption for compliance verification. [83][84][85] Secondly, bioactive compounds in cranberries that are responsible for preventing UTI need to be identified and their mechanisms elucidated. A-type proanthocyanidins from cranberries inhibited the adherence of uropathogenic E. coli in vitro 82 however, their concentration in urine was too low to have such effects.…”

Section: Future Directionmentioning

confidence: 99%

American cranberries and health benefits – an evolving story of 25 years

Zhao

Liu²,

2018

J Sci Food Agric

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Cranberries contain various types of bioactive components. Scientists have been studying cranberries' beneficial effects on urinary tract health since the 20th century. In the 21st century, the protection provided by cranberry phytochemicals against cancer and vascular diseases has drawn more attention from researchers. Anthocyanins, procyanidins, and flavonols in cranberries were all documented to have potential effects on cancer prevention. The cardiometabolic effects of cranberries have been investigated in several clinical trials. It was found that cranberries positively affect atherosclerotic cholesterol profiles and that they reduced several cardiometabolic risk factors. Nowadays, growing evidence suggests other important roles of cranberries in maintaining digestive health. Cranberry juice or cranberries have been shown to inhibit the colonization of H. pylori in stomach, and protect against intestinal inflammation. For future research, clinical trials with improved study design are urgently needed to demonstrate cranberries' benefits on urinary tract health and cardiometabolic diseases. Hypothesis-driven studies using animals or cell culture are needed to elucidate the mechanisms of cranberries' effects on digestive health. © 2018 Society of Chemical Industry.

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“…Therefore, metabolomics, the multi‐targeted analysis of endogenous metabolites from biological samples, is commonly used for biomarker discovery and to provide insight into the mechanisms underlying the observations (Johnson, Ivanisevic, & Siuzdak, ; Ma et al, ). 1 H nuclear magnetic resonance ( 1 H NMR)‐based metabolomics had been proven to very efficiently identify the metabolic consequences of complex biological responses to the intervention of both endogenous (Drenos et al, ) and exogenous factors, such as natural dietary component effects (Liu, Tayyari, Edison, Su, & Gu, ). A urine sample would be the potential ideal candidate for detection of global metabolic responses because of the ease of sample collection and the high number and intensity of altered metabolites (Roux, Thevenot, Seguin, Olivier, & Junot, ).…”

Section: Introductionmentioning

confidence: 99%

“…component effects (Liu, Tayyari, Edison, Su, & Gu, 2016). A urine sample would be the potential ideal candidate for detection of global metabolic responses because of the ease of sample collection and the high number and intensity of altered metabolites (Roux, Thevenot, Seguin, Olivier, & Junot, 2015).…”

mentioning

confidence: 99%

“…A urine sample would be the potential ideal candidate for detection of global metabolic responses because of the ease of sample collection and the high number and intensity of altered metabolites (Roux, Thevenot, Seguin, Olivier, & Junot, 2015). Therefore, the application of urinary 1 H NMRbased metabolomics, a noninvasive method, and interpretation of complex spectral data sets make it possible to analyze several samples, detect differences between physiologically distinct conditions, and supervise dietary components-induced perturbations (Kumar, Gupta, & Nath, 2016;Liu et al, 2016).…”

mentioning

confidence: 99%

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¹H NMR-based metabolic investigation of the effect ofLentinula edodes-derived polysaccharides on aged mice

Yang

Zheng-xiang

et al. 2017

Journal of Food Biochemistry

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Few comprehensive metabolic profiles of Lentinula edodes‐derived polysaccharides on mammalians have been reported to date. In previous studies, a novel heteropolysaccharide, named L2, obtained from L. edodes had shown anti‐ageing effects in mice. In this study, a 1H NMR‐based metabolomics approach was used to investigate the urinary metabolome changes in aged mice gavaged with L2 for better understanding of anti‐ageing mechanism. Six metabolites in urine were identified as potential markers for L2 treatment. An increase in α‐oxoglutarate (α‐OG) and a decrease in β‐hydroxybutyrate, glutamate, glucose, choline, and lactate were observed. Enrichment pathway analysis indicated that 11 metabolic pathways were disrupted after L2 treatment. These altered metabolic pathways are mainly associated with energy metabolism, amino acid metabolism, and glycolysis. The results suggest that L2 could exert anti‐ageing effects partly by regulating these pathways. Moreover, these findings may help better understand the mechanisms of the bioactivities of L. edodes‐derived polysaccharides. Practical applications In this study, a 1H NMR‐based metabolomics approach was used to investigate the urinary metabolome changes in aged mice gavaged with a novel heteropolysaccharide L2 from Lentinula edodes. The findings may help better understand the mechanisms of bioactivities of L. edodes‐derived polysaccharides, and used for further development of L2 in food and medicine.

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NMR-based metabolomics reveals urinary metabolome modifications in female Sprague–Dawley rats by cranberry procyanidins

Cited by 23 publications

References 35 publications

Identification and Quantification by Targeted Metabolomics of Antibiotic-Responsive Urinary Small Phenolic Molecules Derived from the Intestinal Microbiota in Mice

Identification and Quantification by Targeted Metabolomics of Antibiotic-Responsive Urinary Small Phenolic Molecules Derived from the Intestinal Microbiota in Mice

American cranberries and health benefits – an evolving story of 25 years

¹H NMR-based metabolic investigation of the effect ofLentinula edodes-derived polysaccharides on aged mice

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NMR-based metabolomics reveals urinary metabolome modifications in female Sprague–Dawley rats by cranberry procyanidins

Cited by 23 publications

References 35 publications

Identification and Quantification by Targeted Metabolomics of Antibiotic-Responsive Urinary Small Phenolic Molecules Derived from the Intestinal Microbiota in Mice

Identification and Quantification by Targeted Metabolomics of Antibiotic-Responsive Urinary Small Phenolic Molecules Derived from the Intestinal Microbiota in Mice

American cranberries and health benefits – an evolving story of 25 years

1H NMR-based metabolic investigation of the effect ofLentinula edodes-derived polysaccharides on aged mice

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¹H NMR-based metabolic investigation of the effect ofLentinula edodes-derived polysaccharides on aged mice