NMR metabolomics of berry quality in sea buckthorn (Hippophae L.)

Wei, Li; Ruan, Chengjiang; Silva, Jaime A. Teixeira da; Guo, Hai Peng; Zhao, Cheng

doi:10.1007/s11032-012-9768-x

Cited by 30 publications

(10 citation statements)

References 32 publications

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“…Traditionally used for hundreds of years in China and Russia for health-related purposes, sea buckthorn has now gained popularity worldwide due to the unique composition of seed and pulp oil and the abundance of bioactive compounds in fruits, seeds, leaves and bark 1 . The red or orange berries of sea buckthorn contain fleshy pulp rich in vitamin C, carotenoids, flavonoids and monounsaturated palmitoleic acid (up to 39%), and a single seed with an oil content of 8–20% 1 , 2 , comprising high levels of linoleic acid (30–42%) and α-linolenic acid (20–39%) 3 , carotenoids, flavonol glycosides 4 , tocopherol and phytosterols 5 , 6 . The bioactive compounds in sea buckthorn products have clinically proven medicinal effects against tumor progression, inflammation, hypertension and gastric ulcers, and can promote wound healing and tissue regeneration 7 – 11 .…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs involved in lipid biosynthesis and seed size in developing sea buckthorn seeds using high-throughput sequencing

Ding

Ruan

Guan

et al. 2018

Sci Rep

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Sea buckthorn is a plant of medicinal and nutritional importance owing in part to the high levels of essential fatty acids, linoleic (up to 42%) and α-linolenic (up to 39%) acids in the seed oil. Sea buckthorn can produce seeds either via the sexual pathway or by apomixis. The seed development and maturation programs are critically dependent on miRNAs. To understand miRNA-mediated regulation of sea buckthorn seed development, eight small RNA libraries were constructed for deep sequencing from developing seeds of a low oil content line ‘SJ1’ and a high oil content line ‘XE3’. High-throughput sequencing identified 137 known miRNA from 27 families and 264 novel miRNAs. The potential targets of the identified miRNAs were predicted based on sequence homology. Nineteen (four known and 15 novel) and 22 (six known and 16 novel) miRNAs were found to be involved in lipid biosynthesis and seed size, respectively. An integrated analysis of mRNA and miRNA transcriptome and qRT-PCR identified some key miRNAs and their targets (miR164d-ARF2, miR168b-Δ9D, novelmiRNA-108-ACC, novelmiRNA-23-GPD1, novelmiRNA-58-DGAT1, and novelmiRNA-191-DGAT2) potentially involved in seed size and lipid biosynthesis of sea buckthorn seed. These results indicate the potential importance of miRNAs in regulating lipid biosynthesis and seed size in sea buckthorn.

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Section: Introductionmentioning

confidence: 99%

Identification of microRNAs involved in lipid biosynthesis and seed size in developing sea buckthorn seeds using high-throughput sequencing

Ding

Ruan

Guan

et al. 2018

Sci Rep

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“…Sample preparation in NMR usually includes a series of steps such as extraction, phase separation, filtration, and dilution. Similar to traditional techniques, NMR is usually suitable for liquid samples or samples that can be completely dissolved in a solvent such as D 2 O, DMSO‐D 6 , CDCl 2 , or C 6 D 6 (Li and others ). Many measurements have been taken to collect signals with suitable signal‐to‐noise ratio and high resolution.…”

Section: Emerging Detection Techniques For Sugarsmentioning

confidence: 99%

Recent Developments in Methods and Techniques for Rapid Monitoring of Sugar Metabolism in Fruits

Sun

et al. 2016

Comp Rev Food Sci Food Safe

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The major sugars in fruits, including glucose, fructose, and sucrose, play a critical role in fruit quality control and maturity evaluation. Many novel methods and techniques such as enzymatic and nonenzymatic biosensors, nuclear magnetic resonance, and near-infrared spectroscopy have been developed to provide the rapid estimation of sugar inversion under different storage conditions. This review provides a description of recent advancements in these technologies for the determination of sugar contents in fruits. The prospects of emerging spectroscopic technologies such as Raman spectroscopy, hyperspectral imaging, and terahertz imaging, for assessing sugars in intact fruits are discussed. The challenges for further developments of these methods and technologies to meet the increasing demand for rapid and on-line quality control are also presented.

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“…SBP is a good source of edible oil. Bioactive oils (2–38%) with high levels of the uncommon fatty acid (FA) palmitoleic acid (approximately 40%) [2–4], carotenoids, flavonol glycosides, and tocopherol [5–7], can be extracted from the orange or red berry pulp.…”

Section: Introductionmentioning

confidence: 99%

RNA-seq data reveals a coordinated regulation mechanism of multigenes involved in the high accumulation of palmitoleic acid and oil in sea buckthorn berry pulp

Ding

Ruan

et al. 2019

BMC Plant Biol

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Background: Sea buckthorn is a woody oil crop in which palmitoleic acid (C16:1n7, an omega-7 fatty acid (FA)) contributes approximately 40% of the total FA content in berry pulp (non-seed tissue). However, the molecular mechanisms contributing to the high accumulation of C16:1n7 in developing sea buckthorn berry pulp (SBP) remain poorly understood. Results: We identified 1737 unigenes associated with lipid metabolism through RNA-sequencing analysis of the four developmental stages of berry pulp in two sea buckthorn lines, 'Za56' and 'TF2-36'; 139 differentially expressed genes were detected between the different berry pulp developmental stages in the two lines. Analyses of the FA composition showed that the C16:1n7 contents were significantly higher in line 'Za56' than in line 'TF2-36' in the mid-late developmental stages of SBP. Additionally, qRT-PCR analyses of 15 genes involved in FA and triacylglycerol (TAG) biosynthesis in both lines revealed that delta9-ACP-desaturase (ACP-Δ9D) competed with 3-ketoacyl-ACPsynthase II (KASII) for the substrate C16:0-ACP and that ACP-Δ9D and delta9-CoA-desaturase (CoA-Δ9D) gene expression positively correlated with C16:1n7 content; KASII and fatty acid elongation 1 (FAE1) gene expression positively correlated with C18:0 content in developing SBP. Specifically, the abundance of ACP-Δ9D and CoA-Δ9D transcripts in line 'Za56', which had a higher C16:1n7 content than line 'TF2-36', suggests that these two genes play an important role in C16:1n7 biosynthesis. Furthermore, the high expressions of the glycerol-3-phosphate dehydrogenase (GPD1) gene and the WRINKLED1 (WRI1) transcription factor contributed to increased biosynthesis of TAG precursor and FAs, respectively, in the early developmental stages of SBP, and the high expression of the diacylglycerol O-acyltransferase 1 (DGAT1) gene increased TAG assembly in the later developmental stages of SBP. Overall, we concluded that increased ACP-Δ9D and CoA-Δ9D levels coupled with decreased KASII and FAE1 activity is a critical event for high C16:1n7 accumulation and that the coordinated high expression of WRI1, GPD1, and DGAT1 genes resulted in high oil accumulation in SBP. Conclusion: Our results provide a scientific basis for understanding the mechanism of high C16:1n7 accumulation in berry pulp (non-seed tissue) and are valuable to the genetic breeding programme for achieving a high quality and yield of SBP oil.

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NMR metabolomics of berry quality in sea buckthorn (Hippophae L.)

Cited by 30 publications

References 32 publications

Identification of microRNAs involved in lipid biosynthesis and seed size in developing sea buckthorn seeds using high-throughput sequencing

Identification of microRNAs involved in lipid biosynthesis and seed size in developing sea buckthorn seeds using high-throughput sequencing

Recent Developments in Methods and Techniques for Rapid Monitoring of Sugar Metabolism in Fruits

RNA-seq data reveals a coordinated regulation mechanism of multigenes involved in the high accumulation of palmitoleic acid and oil in sea buckthorn berry pulp

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