NMR Profiling of Ononis diffusa Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines

Graziani, Vittoria; Potenza, Nicoletta; D’Abrosca, Brigida; Troiani, Teresa; Napolitano, Stefania; Fiorentino, Antonio; Scognamiglio, Monica

doi:10.3390/molecules26113266

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Cetuximab, an antibody inhibiting the EGFR signaling pathway, is employed in the treatment of colorectal cancer [45]. While SW48 and CaCO2 colorectal cancer cells exhibit sensitivity to cetuximab, HT29 cancer cells display resistance [46]. Our cytotoxicity studies with benzoic acid yielded similar findings to cetuximab.…”

Section: Discussionsupporting

confidence: 64%

The Cytotoxic Effect of Benzoic Acid on Ten Different Cancer Cell Lines

Öztürkel Kabakaş,

Sezer Kürkçü,

Onat Taşdelen

et al. 2024

Eskişehir Technical University Journal of Science and Technology a - Applied Sciences and Engineering

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Cancer, having numerous types, is among the most dangerous and complex chronic diseases in the world affecting the wellbeing of humans, society and economy. The exploration and reassessment of effective chemicals, compounds, and natural products as potential agents for alleviating the adverse effects of cancer and its related symptoms continue on a global scale. This process involves an initial evaluation of the cytotoxic activities of potential drug candidates or treatment regimens on diverse cancer cell types in an ex vivo context. Benzoic acid (BA), an aromatic carboxylic acid that is widely available and used in the food industry, is one of the phenolic acids that may bear considerable anti-cancer potential. It is useful to find out the comparable effect of BA on various cancer types. Therefore, in this study, we tested the cytotoxicity of BA using MTT assay, on a number of ten different cancer cell lines and one normal cell type, namely prostate cancer (PC3), cervical cancer (HeLA), liver cancer (HUH7), colon cancer (CaCO2, HT29, SW48), bone cancer (MG63 and A673), pharyngeal cancer (2A3), lung cancer (CRM612) and kidney epithelial control cell line (Phoenix), respectively. IC50 (µg/ml) values after 48 and 72-hour exposure to BA were found to differ between 85.54±3.17 to 670.6±43.26, while the IC values for the control cell line Phoenix were 410.54±32.29 and 231.16±25.25, respectively. Taking into account of statistical evaluation of the IC50 values for BA on 11 cell types, we suggest that the molecular and omics approaches can be implemented in more details in order to find cellular and biochemical targets of BA as well as elucidating molecular mode of action, especially starting with the cancer cell lines of MG63, CRM612 and A673, in which the IC50 levels are relatively the lowest compared to those of the control cell line.

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Section: Discussionsupporting

confidence: 64%

The Cytotoxic Effect of Benzoic Acid on Ten Different Cancer Cell Lines

Öztürkel Kabakaş,

Sezer Kürkçü,

Onat Taşdelen

et al. 2024

Eskişehir Technical University Journal of Science and Technology a - Applied Sciences and Engineering

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show abstract

“…Graziani et al employed NMR-based chemical profiling of Ononius diffusa to identify cytotoxic compounds with activity against colorectal cancer cell lines. This approach was found to be much more successful, in terms of time and costs in the detection and identification of bioactive compounds, as compared to classical bioactivity-guided fractionation [ 7 ]. A similar design, integrating chemical profiling with bioactivity tests, allowed Nipun et al [ 8 ] to identify α-glucosidase inhibitors from Psychotria malayana leaves using an LC-MS platform [ 8 ].…”

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confidence: 99%