NMR studies of pH-induced transport of carboxylic acids across phospholipid vesicle membranes

Cramer, John A.; Prestegard, James H.

doi:10.1016/0006-291x(77)91042-7

Cited by 62 publications

(28 citation statements)

References 3 publications

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“…Undissociated organic acids can permeate the cell membrane by diffusion and release protons in the cell. The influx of protons is thought to induce acidification of the cytoplasm and dissipate the membrane proton potential (⌬pH) (6,9,10). In a recent report, we demonstrated that L. casei strain Shirota exerts a bactericidal effect on STEC strains during coculture and that this effect is dependent on the lactic acid produced by L. casei during culture (M. Ogawa, K. Shimizu, K. Nomoto, R. Tanaka, T. Hamabata, S. Yamasaki, T. Takeda, and Y. Takeda, submitted for publication).…”

Section: Discussionmentioning

confidence: 99%

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

et al. 2001

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Section: Discussionmentioning

confidence: 99%

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

et al. 2001

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“…and dissipate the membrane proton potential (6,10,13). This leads to the disruption of the proton motive force and the inhibition of substrate transport mechanisms, energy-yielding processes, and macromolecule synthesis (7,12).…”

Section: Vol 72 2004 Probiotics Protect Mice From Stec Infection 2245mentioning

confidence: 99%

Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7

et al. 2004

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The anti-infectious activity of probiotic Bifidobacteria against Shiga toxin-producing Escherichia coli (STEC) O157:H7 was examined in a fatal mouse STEC infection model. Stable colonization of the murine intestines was achieved by the oral administration of Bifidobacterium breve strain Yakult (naturally resistant to streptomycin sulfate) as long as the mice were treated with streptomycin in their drinking water (5 mg/ml). The pathogenicity of STEC infection, characterized by marked body weight loss and subsequent death, observed in the infected controls was dramatically inhibited in the B. breve-colonized group. Moreover, Stx production by STEC cells in the intestine was almost completely inhibited in the B. breve-colonized group. A comparison of anti-STEC activity among several Bifidobacterium strains with natural resistance to streptomycin revealed that strains such as Bifidobacterium bifidum ATCC 15696 and Bifidobacterium catenulatum ATCC 27539 T did not confer an anti-infectious activity, despite achieving high population levels similar to those of effective strains, such as B. breve strain Yakult and Bifidobacterium pseudocatenulatum DSM 20439. The effective strains produced a high concentration of acetic acid (56 mM) and lowered the pH of the intestine (to pH 6.75) compared to the infected control group (acetic acid concentration, 28 mM; pH, 7.15); these effects were thought to be related to the anti-infectious activity of these strains because the combination of a high concentration of acetic acid and a low pH was found to inhibit Stx production during STEC growth in vitro.

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“…Optimal antifungal activity takes place at low pH and near pK since it favors the undissociated form of the acid that is freely permeable across the plasma membrane microorganisms (Bogaert & Naidu, 2000). The neutral form diffuses across the fungal cytoplasmic membrane (Cramer & Prestegard, 1977) and dissociates due to a higher pH and the preservative action is thought to be due to the accumulation of anions and protons inside the cell (Brul & Coote, 1999;Jay, 2000). Protons released, destabilize the neutral intracellular pH and break the cell membrane gradient (Salmond, Kroll, & Booth, 1984).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory activity of lactic and acetic acid on Aspergillus flavus growth for food preservation

et al. 2012

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NMR studies of pH-induced transport of carboxylic acids across phospholipid vesicle membranes

Cited by 62 publications

References 3 publications

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

Protective Effect ofLactobacillus caseiStrain Shirota on Shiga Toxin-ProducingEscherichia coliO157:H7 Infection in Infant Rabbits

Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7

Inhibitory activity of lactic and acetic acid on Aspergillus flavus growth for food preservation

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