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Matsushita, Ryo; Hattori, Kenji; Hayashi, Kaori; Iizasa, Hisashi; Ichimura, Fujio; Nakashima, Emi

doi:10.1023/a:1011048905732

Cited by 3 publications

(3 citation statements)

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“…21) Without IND, ADM or CDDP, the tumor cells were rejected by the mice that were reinoculated with tumor cells (Table 1). Antitumor immunity was also observed against MCP-1 transfectant when ADM, BLM or CDDP was administered in combination with IND.…”

Section: Resultsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

“…[18][19][20] We have shown that IND and bleomycin (BLM) exhibit a strong synergistic effect. 21) In this report, we carried out further investigations of a combination of IND with adriamycin (ADM) or cisplatin (CDDP), which have different potencies and sites of action compared with BLM.Previously, we have shown that monocyte chemotactant protein-1 (MCP-1) acts synergistically with lipopolysaccharide (LPS) on tumor growth.22) Therefore, we also examined the possibility that MCP-1 expression may influence the synergistic antitumor effects of IND and chemotherapeutic drugs. …”

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Synergistic Effect of Indomethacin with Adriamycin and Cisplatin on Tumor Growth.

Hattori¹,

Matsushita

Kimura

et al. 2001

Biological & Pharmaceutical Bulletin

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Prostaglandins (PGs) are known to be one of the regulators of tumor growth and spread.1-3) PGs facilitate tumor growth by suppressing the immune system 4-6) while indomethacin (IND), an inhibitor of PG production, has been reported to suppress tumor growth in some animal tumor models, [1][2][3][7][8][9][10][11][12] and several reports have suggested that IND facilitates tumors. [13][14][15][16] Although the antitumor efficacy of IND remains controversial, Tanaka et al. reported that IND given to mice with large burdens of colon 26 alleviated the cachexia in these animals, resulting in an prolonged survival time, even although the tumor growth was promoted. 17)These findings suggest that IND may restore tumor-induced disorders in homeostasis. Several studies have been reported showing that IND exhibits synergistic effects with several other biological response-modifiers and chemotherapeutic drugs in transplanted tumor models. [18][19][20] We have shown that IND and bleomycin (BLM) exhibit a strong synergistic effect. 21) In this report, we carried out further investigations of a combination of IND with adriamycin (ADM) or cisplatin (CDDP), which have different potencies and sites of action compared with BLM.Previously, we have shown that monocyte chemotactant protein-1 (MCP-1) acts synergistically with lipopolysaccharide (LPS) on tumor growth.22) Therefore, we also examined the possibility that MCP-1 expression may influence the synergistic antitumor effects of IND and chemotherapeutic drugs. MATERIALS AND METHODSMaterials IND was obtained from Wako Pure Chemical, Ind. (Tokyo, Japan), BLM and CDDP were purchased from Nihon Kayaku (Tokyo, Japan), ADM was purchased from Kyowa Hakko (Tokyo, Japan) and all other chemicals used were of reagent grade.Mice Female BALB/c mice (8-weeks old) were obtained from Clea (Tokyo, Japan). They were maintained under specific pathogen-free conditions and their body weight was measured twice a week between 9:00 and 11:00 a.m. All animal experiments were approved by the Institutional Animal Care and Use Committee and complied with the standards set out in the Guidelines for the Care and Use of Laboratory Animals on the Takara-machi Campus of Kanazawa University.Cell Culture Clone 20 (C20) is a subclone of the colon 26 adenocarcinoma cell line which induces severe cancer cachexia. We previously established a cell line from C20, transfected MCP-1, designated C20bA-2-1.22) These cell lines were grown as monolayer cultures in complete medium consisting of RPMI 1640 medium supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 units/ml penicillin G, and 100 mg/ml streptomycin at 37°C with 5% CO 2 .Inoculation of Tumor Cells The adherent cells were collected after a brief period of trypsinization and counted. Nine-week-old female mice were inoculated in the footpad of the right hind limb with 1ϫ10 5 tumor cells per mouse suspended in 0.04 ml sterilized endotoxin-free PBS. The tumor size at the injected site was determined by measuring the footpad thickness with calipers. At day 1 aft...

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Section: Resultsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

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confidence: 99%

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