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Jakobsson, Staffan; Borg, Bertil; Haux, Carl; Hyllner, Sven Johan

doi:10.1023/a:1007776016610

Cited by 120 publications

(19 citation statements)

References 22 publications

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“…The apparent differences between the predicted and observed molecular masses could be accounted for by post-translational modifications as up to four N-glycosylation sites and one O-glycosylation site were predicted (20) by their deduced amino acid sequences. This is consistent with our previous investigation where spiggin assayed positively for the presence of carbohydrate (6). Conversely, only a single distinct protein band of 203 kDa was detected within the urinary bladder of male sticklebacks that also concurred with our previous investigations (6).…”

Section: Discussionsupporting

confidence: 93%

“…This is consistent with our previous investigation where spiggin assayed positively for the presence of carbohydrate (6). Conversely, only a single distinct protein band of 203 kDa was detected within the urinary bladder of male sticklebacks that also concurred with our previous investigations (6). These observations suggest that formation of spiggin involves multimerization of the constituent kidney subunits in the urinary bladder.…”

Section: Discussionsupporting

confidence: 92%

“…Effects of Steroid Treatments-Steroid treatments were performed using adult females, as their kidneys do not undergo hypertrophy under natural conditions (6). Nonbreeding females housed at 9°C and a photoperiod of 8:16 h light:dark were anesthetized with 0.1% (v/v) 2-phenoxyethanol (Sigma) and implanted intraperitoneally with Silclear silicone tubing (10-mm length, 0.6-mm inner diameter, 1.2-mm outer diameter) containing steroids dissolved in cocoa butter.…”

Section: Methodsmentioning

confidence: 99%

“…Previously we have characterized spiggin from the urinary bladder content of male sticklebacks as being a 203-kDa cysteine-rich glycoprotein (6). No similarities in amino acid composition could be observed between spiggin and murine kidney androgen-regulated protein, elastin, collagen, fibroin, or vitelline envelope proteins (7)(8)(9).…”

mentioning

confidence: 98%

“…However, a distinct example occurs in the teleostean stickleback family (Gasterosteidae) where the secondary proximal epithelium cells of the male kidney undergo hypertrophy during the breeding season (5). In male three-spined stickleback (Gasterosteus aculeatus) this structural reorganization is due to the synthesis of an adhesive protein called "spiggin," which is under the regulation of 11-ketoandrogens (6). This agglutinate is subsequently secreted into the urinary bladder for storage and employed as a structural and highly elastic adhesive thread to assemble a nest from plant material in which the female lays her eggs (5,6).…”

mentioning

confidence: 99%

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Molecular Cloning and Characterization of Spiggin

Jones

Lindberg

Jakobsson

et al. 2001

Journal of Biological Chemistry

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One of the most definitive examples of a vertebrate extraorganismal structural protein can be found in three-spined sticklebacks (Gasterosteus aculeatus). In the breeding male the kidney hypertrophies and synthesizes an adhesive protein called "spiggin," which is secreted into the urinary bladder from where it is employed as a structural thread for nest building. This paper describes the first molecular characterization of spiggin and demonstrates that this adhesive is a protein complex assembled from a potential of three distinct subunits (␣, ␤, and ␥). These subunits arise by alternative splicing, and 11-ketoandrogens induce their expression in stickleback kidneys. Analysis of the predicted amino acid sequence of each subunit reveals a modular organization whose structural elements display a similarity to the multimerization domains found within von Willebrand Factor-related proteins. These results implicate that spiggin utilizes a conserved multimerization mechanism for the formation of a viscous agglutinate from its constituent subunits in the urinary bladders of male sticklebacks. This novel extraorganismal structural protein is therefore ideally suited to its function as an adhesive thread.Extrorganismal proteins are common among invertebrates. Examples range from the fibroin threads forming spider webs and silkworm cocoons (1, 2) to the collagen-based matrixes of the byssus threads of Mytilus and the Drosophila sgs family (3, 4).

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Molecular Cloning and Characterization of Spiggin

Jones

Lindberg

Jakobsson

et al. 2001

Journal of Biological Chemistry

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Extra‐Organismic Adhesive Proteins

2003

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Introduction Occurrence Barnacles Algae Mussels Molecular Genetics Functional Biomechanics Measurement of Adhesion Strength Applications of Mussel Adhesive Proteins Mucoadhesion and Surface Chemistry Corrosion Inhibitors Synthetic Biomimics Outlook and Perspectives Patents

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