nNOS is involved in cardiac remodeling induced by chronic ethanol consumption

Silva, Susana Maria; Silva, Sérgio; Meireles, Manuela; Leal, Sandra

doi:10.1016/j.tox.2015.01.009

Cited by 6 publications

(8 citation statements)

References 44 publications

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“…The blood alcohol concentrations presented herein are comparable to those previously observed in human [40] and in rats [13, 14, 30, 38, 40]. Our results also show that ET rats ingested less liquid and had lighter kidneys than Control rats, corroborating a previous study that showed that ethanol consumption induces a decrease of urine volume and liquid intake [6].…”

Section: Discussionsupporting

confidence: 92%

“…Heavy ethanol consumption effects on BP were predictable on the basis of data from our [14] and others studies [8, 12]. In the present study, we extend those observations by showing that the hypertensive effect of ethanol consumption was not completely reversed after long-term ethanol withdrawal.…”

Section: Discussionsupporting

confidence: 87%

“…The daily amount of food and liquid intake by Control, ET and W rats is in line with previous research [13, 14, 30, 38] in which the same experimental model of alcohol consumption and withdrawal was used. The blood alcohol concentrations presented herein are comparable to those previously observed in human [40] and in rats [13, 14, 30, 38, 40].…”

Section: Discussionsupporting

confidence: 83%

“…The animals were submitted to an adaptation period during one week before measurements. BP measurements were recorded during the last two weeks of ethanol and withdrawal [14]. …”

Section: Methodsmentioning

confidence: 99%

“…Protein extraction and protein concentration determination were performed as previously described [14] using whole kidney homogenates. Immunoblotting was performed with the same rabbit anti-AT1R antibody at 1: 500 dilution and mouse anti-α-tubulin (T6199, RRID: AB_477583; Sigma-Aldrich, Madrid, Spain) at 1: 1000 dilution, followed by incubation with anti-rabbit (Sigma-Aldrich, Madrid, Spain) and anti-mouse (Vector Laboratories, Burlingame, CA) secondary antibodies conjugated to horseradish peroxidase at 1: 1000 dilution.…”

Section: Methodsmentioning

confidence: 99%

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Heavy Alcohol Consumption Effects on Blood Pressure and on Kidney Structure Persist After Long-Term Withdrawal

Leal

Jorge

Barbosa

et al. 2017

Kidney Blood Press Res

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Background/Aims: Heavy ethanol consumption is a risk factor for hypertension and prompts organ damage. There is no information regarding the impact of long-term heavy ethanol consumption on kidney structure and function linking to their hypertensive effects nor the repercussions after withdrawal. Methods: Rats were exposed to ethanol for 24 weeks and, afterwards, a group was assigned to withdrawal for 8 weeks. Blood pressure (BP) was measured and serum biochemical parameters were quantified. Glomerular volume density, areal density of glomerular tuft and renal corpuscles were determined. Angiotensin II type 1 receptor (AT1R) protein expression was evaluated. Results: Twenty-four weeks of ethanol consumption causes atrophy of renal corpuscles and glomeruli and reduces the volume of glomeruli. Glomerular changes induced by ethanol consumption were still evident after withdrawal. Renal AT1R levels were increased in ethanol-treated rats and returned to control levels during withdrawal. Ethanol consumption also induced an increase in BP, uric acid and albumin levels. Upon withdrawal, systolic and mean arterial pressures decreased, but were still higher than in controls rats. Conclusion: Ethanol consumption induces changes in glomerular morphology associated with increased BP and AT1R expression. Long-term withdrawal was inefficient to restore the structural integrity of renal corpuscles and in lowering systolic pressure.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 83%

“…The animals were submitted to an adaptation period during one week before measurements. BP measurements were recorded during the last two weeks of ethanol and withdrawal [14]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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