No clinically relevant differences between capillary and venous blood cell counts in adult haematological patients using a nonautomated lancet

Herrera-Rojas, Miguel Ángel; Tarín‐Arzaga, Luz; León, Andrés Gómez‐De; Larracoechea, Alberto Vázquez-Mellado de; Jaime‐Pérez, José Carlos; Cantú-Rodrı́guez, Olga Graciela; Gutiérrez-Aguirre, César Homero; Salazar-Riojas, Rosario; Valdes-Galvan, Mayra Judith; Gómez‐Almaguer, David

doi:10.1111/ijlh.12340

Cited by 6 publications

(13 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to our study, they found no clinically significant differences between these tubes for CBC use [16]. In the literature, there are several studies that compare capillary blood taken from a SVT and venous blood taken from a vacuum tube; however, the results are conflicting [17][18][19]. In our study, venous blood collection was used for both tubes.…”

Section: Discussioncontrasting

confidence: 94%

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

Ünal¹

2019

J Med Biochem

View full text Add to dashboard Cite

Comparison of small-volume tubes and vacuum blood tubes for complete blood count T he complete blood count (CBC) is one of the most commonly requested clinical laboratory tests [1]. Accurate measurement of the CBC is essential for the correct diagnosis, treatment, and follow-up of patients with hematological disorders. These measurements are now almost fully automated, and the right interpretation of the clinical significance of the results requires knowledge of the pre-analytical phases [2]. The blood collection tubes used for a CBC should contain anticoagulant ethylenediaminetetraacetic acid (EDTA), according to the recommendation of the Clinical & Laboratory Standards Institute (CLSI) [3]. EDTA salts are used for the anticoagulation of whole blood for hematological analyses because the cellular components of the blood are well preserved by EDTA [4]. Today, a CBC is typically performed with whole blood collected in tubes containing dipotassium EDTA (K2EDTA) as an anticoagulant. Blood collection tube additives and components are an important component of the pre-analytical phase in terms of a potential influence on laboratory test results. Although Objectives: A complete blood count (CBC) is one of the most commonly requested clinical laboratory tests. Vacuum blood tubes are used routinely, and now, new small-volume tubes (SVTs) containing dipotassium ethylenediaminetetraacetic acid (K2EDTA) are also in use. The aim of this research was to compare SVTs with vacuum blood tubes for use in a CBC. Methods: Venous blood samples were taken from 40 healthy volunteers and were collected in BD Vacutainer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) K2EDTA tubes and BD Microtainer (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) K2EDTA tubes. CBC parameters were analyzed using an ABX Pentra DF 120 device (Horiba, Ltd., Kyoto, Japan). Results: Red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and basophil (BASO) levels were found to be statistically significantly higher, while platelet (PLT) levels were determined to be statistically significantly lower in the SVT analyses compared with those of the vacuum blood tubes. When the percentage difference was compared with the total allowable error, RBC, HGB, HCT, MCH, MCHC, red cell distribution width, white blood cell count, neutrophil, lymphocyte, monocyte, eosinophil, and BASO values demonstrated a general trend of positive bias, while PLT values demonstrated a general trend of negative bias on a Bland-Altman bias plot. Conclusion: The results of this study demonstrated that venous blood samples from SVTs generated a significant bias in the CBC results. In addition, clotted samples were observed more frequently among those collected in SVTs in this study. The design of SVTs may have an important and detrimental effect on the accuracy and efficacy of the results of a CBC.

show abstract

Section: Discussioncontrasting

confidence: 94%

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

Ünal¹

2019

J Med Biochem

View full text Add to dashboard Cite

show abstract

“…During the incremental cycling tests, 95 KL capillary blood samples were collected immediately before, 5 and 7 min after rebreathing whereas in the RSA tests, a 2-mL venous blood sample was taken immediately before and 6 min after rebreathing. The different methods are believed to have a negligible effect on tHb-mass measurement (25). HbCO% also was measured directly after the RSA test but not after the incremental exercise test and so an estimation of the HbCO% during the last 2 min was made (26).…”

Section: Thb-mass Determination/co Administrationmentioning

confidence: 99%

The Performance Effects of Microdose Recombinant Human Erythropoietin Administration and Carbon Monoxide Rebreathing

Sutehall

Martin‐Rincon

Wang

et al. 2018

Curr Sports Med Rep

View full text Add to dashboard Cite

Frequent, low doses of recombinant human erythropoietin (rHuEpo) have been shown to increase the oxygen carrying capacity of an athlete and enhance endurance performance, although its effect on repeated sprint ability (RSA) remains unknown. If the mechanisms behind improved RSA performance reside within the augmented O 2 carrying capacity, then carbon monoxide (CO) inhalation should inhibit RSA. Purpose: The aim of this study was to assess the effects on maximal oxygen uptake (VO 2max ) and RSA of two interventions known to differentially influence blood oxygen carrying capacity. Methods: Fourteen endurance-trained individuals were administered microdoses of rHuEpo (20Y40 IU . kg j1 ) or placebo twice per week for 7 wk using a randomized, crossover design. VO 2max and RSA were measured at baseline and after rHuEpo administration. Total hemoglobin mass (tHb-mass) was measured twice at baseline (14 and 7 d before the first injection), three times during rHuEpo administration (10, 24, and 38 d after the first rHuEpo injection) and twice after the cessation of rHuEpo administration (7 and 21 d after the final injection) using the optimized CO rebreathing method. VO 2max and RSA also were assessed in a separate cohort of 11 Black lines represent the mean for all subjects. (A) Changes in VO 2max following rHuEpo administration, (B) VO 2max following placebo and CO rebreathing, (C) Changes in VT following rHuEpo administration and (D) Changes in VT following placebo or CO rebreathing.

show abstract

“…The concordance between measurements of fingerprick and venous blood samples using a standard hematology analyzer had been reported to be high, in another study evaluating the performance of a novel microfluidic impedance cytometer for CBC at the point‐of‐care (POC) 2 . No clinically relevant differences between CB and VB cell counts in adult hematological patients, using nonautomated lancet versus automated, have been also shown in a more recent study 7 . In the present study, although the higher CB values of Hb, Hct, WBC, RBC, MCV, and MCH and the lower CB values of Plt were confirmed in both analyzers, the differences were clinically nonsignificant, as it is easily concluded, comparing the median CB versus VB values of all parameters.…”

Section: Discussionmentioning

confidence: 93%

“…In a previous study, capillary hemoglobin (Hb), hematocrit (Hct), white blood cells (WBC), red blood cells (RBC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH) were all found significantly higher than the respective venous values, while Hb and platelet screening seemed equivalent with either CB or VB, using a hematology analyzer 4 . No clinically relevant differences between CB and VB cell counts in adult hematological patients, using a nonautomated lancet, have been shown by a different study 7 …”

Section: Introductionmentioning

confidence: 89%

“…4 No clinically relevant differences between CB and VB cell counts in adult hematological patients, using a nonautomated lancet, have been shown by a different study. 7 In this study, we assessed CB counts on classical hematology analyzers, as potential substitutes of VB counts, with an intention it to be applied in the daily decisions, like chemotherapy schedule modification and transfusion, in hospitalized patients. We compared simultaneously CB and VB counts on two different hematology analyzers, and we estimated the performance characteristics (sensitivity, specificity) of CB counts on clinical decision-making, using the measurements on hematology analyzers as reference measurements.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of automated capillary complete blood counts for routine clinical decision making in a large cohort of hematological patients, using Mindray BC‐3000 Plus Auto and Sysmex XE‐5000 hematology analyzers

Oudatzis¹,

Tsagarakis²,

Paterakis³

et al. 2020

Int J Lab Hematology

View full text Add to dashboard Cite

Introduction Venous blood (VB) sampling for complete blood count (CBC) via venipuncture is the basic method for the daily evaluation of hematological patients. However, several issues during this process, such as venipuncture difficulty and repetitive attempts, may cause pain, phlebitis, hematomas, inadequate sampling, and patient discomfort. Capillary blood (CB) sampling could be an alternative and less painful solution for the patient. The purpose of this study was the comparative evaluation of basic CBC parameters, as counted from venous and capillary blood samples. Methods During the period 06/2016‐06/2019 in which the study was conducted, 1634 automated counts of VB or CB were performed, derived from 425 hematological hospitalized patients. Bland‐Altman plots were performed to show the agreement of VB and CB counts of common hematological parameters (Hb, Hct, WBC, absolute neutrophil count‐[ANC], RBC, Plt, MCV, MCH), using two different hematology analyzers (Mindray BC‐3000 Plus Auto and Sysmex XE‐5000). Clinical significance of CB sampling was assessed by applying specific clinically significant cutoffs for Hb, ANC, and Plt. Results All measured parameters revealed a significant correlation (r > .9) between CB and VB samples, irrelatively of the hematology analyzer used. CB measurements of Hb, ANC, and Plt, at different clinically important cutoff levels, showed excellent sensitivity (87%‐100%), specificity (95%‐100%), positive predictive value, and negative predictive value (87%‐100% and 90%‐100%, respectively). Conclusion Capillary blood and VB counts in hematological patients were equivalent for most basic hematological parameters. Hb, ANC, and Plt CB counts revealed clinically significant performance, indicating that they can reliably substitute VB sampling in the day work.

show abstract

No clinically relevant differences between capillary and venous blood cell counts in adult haematological patients using a nonautomated lancet

Cited by 6 publications

References 8 publications

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

Comparison of small-volume tubes with vacuum blood tubes for complete blood count

The Performance Effects of Microdose Recombinant Human Erythropoietin Administration and Carbon Monoxide Rebreathing

Evaluation of automated capillary complete blood counts for routine clinical decision making in a large cohort of hematological patients, using Mindray BC‐3000 Plus Auto and Sysmex XE‐5000 hematology analyzers

Contact Info

Product

Resources

About