No Detection of Streptococcus gallolyticus and Helicobacter pylori in Colorectal Cancer Tissue Samples in Shiraz, Iran

Mahmoudvand, Shahab; Zamani, Khosrow; Safaei, Akbar; Khashei, Reza; Motamedifar, Mohammad; Azizi, Zohre; Sarvari, Jamal

doi:10.17795/ijcp-6337

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…However, none of our patients had a history of bacteremia/bacterial-endocarditis and this may explain the lack of difference in abundance of S. gallolyticus between two groups. In agreement with our results, Mahmoudvand et al (2017) reported no association between S. gallolyticus and colorectal cancer in paraffin-embedded biopsy specimens [ 20 ]. Only in studies conducted by Abdulamir et al (2010) and Farajzadeh Sheikh et al (2020), S. gallolyticus was detected with higher frequency in CRC patients with and without a history of bacterial endocarditis/bacteremia compared to healthy controls [ 21 , 22 ].…”

Section: Discussionsupporting

confidence: 93%

High frequency of enterotoxigenic Bacteroides fragilis and Enterococcus faecalis in the paraffin-embedded tissues of Iranian colorectal cancer patients

et al. 2021

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Background The association between specific bacteria and colorectal cancer (CRC) has been proposed. Only a few studies have, however, investigated this relationship directly in colorectal tissue with conflicting results. So, we aimed to quantitate Streptococcus gallolyticus, Fusobacterium spp, Enterococcus faecalis and enterotoxigenic Bacteroides fragilis (ETBF) in formalin-fixed and paraffin-embedded (FFPE) colorectal tissue samples of Iranian CRC patients and healthy controls. Methods A total of 80 FFPE colorectal tissue samples of CRC patients (n = 40) and healthy controls (n = 40) were investigated for the presence and copy number of above bacterial species using quantitative PCR. Relative quantification was determined using ΔΔCT method and expressed as relative fold difference compared to reference gene. Results Relative abundance and copy number of E. faecalis and ETBF were significantly higher in CRC samples compared to control group. E. faecalis was more prevalent than ETBF in tumor samples. Frequency of ETBF and E. faecalis in late stages (III/IV) of cancer was significantly higher than early stages (I/II). We did not detect a significant difference in abundance of S. gallolyticus and Fusobacterium spp between two groups. Conclusion Our study revealed the higher concentration of E. faecalis and ETBF in FFPE samples of CRC patients than controls. However, additional investigations on fecal and fresh colorectal cancer tissue samples are required to substantiate this correlation.

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Section: Discussionsupporting

confidence: 93%

High frequency of enterotoxigenic Bacteroides fragilis and Enterococcus faecalis in the paraffin-embedded tissues of Iranian colorectal cancer patients

et al. 2021

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“…Interestingly, we found that 60% of the patients with CRC were infected with Sgg (P = 0.006). In contrast, Mahmoudvand et al reported 0% (0 out of 6) in colorectal adenocarcinoma biopsy tissues [14]. The differences between these studies could be explained by different population genetics or socioeconomic backgrounds of the studied cohorts.…”

Section: Resultsmentioning

confidence: 83%

“…DNA was stored in a freezer at − 20 °C until used. DNA extraction and PCR controls were the β-globin sequence as previously described [14]. To design the PCR primers, the DNASTAR Lasergene software (https://www.dnastar.…”

Section: Methodsmentioning

confidence: 99%

“…Understanding the mechanisms underlying CRC development requires sophisticated studies. Many studies, including recent meta-analyses have shown that Sgg is more common in patients with CRC than in healthy subjects although contradictory studies also abound [12][13][14][15][16]. Collective in vitro knowledge implicates Sgg in the CRC development because they are thought to enhance the proliferation of cancer cells through the β-catenin signaling pathway as studied in the murine colon cells [6].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Streptococcus gallolyticus in tumor samples of Iranian patients diagnosed with colorectal cancer

Kamali

Abadi

et al. 2022

BMC Res Notes

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Objective Clinical outcomes of infection by S. gallolyticus have not been investigated extensively. We aimed to determine the prevalence of S. gallolyticus in tumor specimens obtained from Iranian patients diagnosed with colorectal cancer. Polymerase chain reaction was used to confirm the presence of S. gallolyticus in patients’ tissue samples. Results Of 176 patients, 65 were diagnosed with colorectal cancer whereas 111 did not have any colon disease. No correlation was found between age, colonization with S. gallolyticus, gender, or risk factors. Overall, 72 (40%) patients carried S. gallolyticus; only 29% of the patients without colorectal cancer were positive for S. gallolyticus. Diagnosis of colorectal cancer and presence of S. gallolyticus significantly correlated (P = 0.006; odds ratio = 1.46; 95% CI = 1.21–3.87). Among the patients with colorectal cancer, 39 (60%) were positive with S. gallolyticus (P = 0.006) whereas 33 of 111 (29.7%) control subjects were positive for S. gallolyticus (P > 0.05); thus, 70.3% of the control subjects were not infected with S. gallolyticus. We found a high prevalence of S. gallolyticus among an Iranian cohort of patients with colorectal cancer. Despite previous reports, we report a positive correlation between colorectal cancer and S. gallolyticus colonization.

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“…Deparaffinization and DNA extraction were done as previously described [ 19 , 20 ]. Polymerase chain reaction (PCR) with consensus primers PCO3/PCO4 (Table 1 ) was performed to amplify β-globin gene fragments to confirm the quality of extracted DNA.…”

Section: Main Textmentioning

confidence: 99%

No detection of EBV, BKV and JCV in breast cancer tissue samples in Iran

et al. 2019

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ObjectiveThe most common cancer amongst women is breast cancer. Reports on the role of EBV, BKV, and JCV in the development of breast cancer are controversial. Hence, the aim of this study was to determine the frequency of EBV, BKV, and JCV in malignant breast tumors in comparison with benign ones.ResultsA total of 300 breast biopsy tissues were included, of which 150 were malignant and 150 benign. After deparaffinization, tissues were subjected to DNA extraction. β-globin gene was amplified by PCR to evaluate the quality of extracted DNA. In house PCRs assay was performed to detect EBV, JCV, and BKV genome fragment. The mean age of malignant and benign groups was 45.0 ± 9.4 and 35.2 ± 12.1 years old. Out of 150 malignant samples, 146 were ductal, two lobular and two samples both invasive ductal and lobular carcinoma. In the benign group, 96, 52 and two samples were fibroadenoma, fibrocystic, and adenosis types, respectively. Genomic DNA fragment of EBV, BKV, and JCV was not found in any of the malignant and benign breast tissues.ConclusionAccording to our finding, there is the possibility that EBV, BKV, and JCV are not involved in breast cancer pathogenesis.

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No Detection of Streptococcus gallolyticus and Helicobacter pylori in Colorectal Cancer Tissue Samples in Shiraz, Iran

Cited by 7 publications

References 26 publications

High frequency of enterotoxigenic Bacteroides fragilis and Enterococcus faecalis in the paraffin-embedded tissues of Iranian colorectal cancer patients

High frequency of enterotoxigenic Bacteroides fragilis and Enterococcus faecalis in the paraffin-embedded tissues of Iranian colorectal cancer patients

Identification of Streptococcus gallolyticus in tumor samples of Iranian patients diagnosed with colorectal cancer

No detection of EBV, BKV and JCV in breast cancer tissue samples in Iran

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