No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix metalloproteinase‐2/9 in osteosarcoma cell lines

Abstract

Pulsed electromagnetic fields (PEMF) could enhance the cytocidal effects of chemotherapeutic drugs on malignant tumor cell lines, but metastasis effects of PEMF on tumor cells have not been investigated. We investigated the effects of PEMF exposure on the expression levels of some metastasis-related molecules, including integrin α subunits (α1, α2, α3, α4, α5, α6, αv), integrin β subunits (β1, β2, β3, β4), CD44, and matrix metalloproteinase-2/9 (MMP-2/9) in four human osteosarcoma cell lines (HOS, MG-63, SAOS-2, NY) and two mouse osteosarcoma cell lines (DOS, LM8) by using FACScan analysis, gelatin zymography, and Western blot analysis. Our results indicate that PEMF exposure has no effect on the expression of some molecules that are associated with tumor cell invasion and metastasis, and therefore suggest that PEMF exposure may be safely applied to chemotherapy for osteosarcoma.

Pulsed Electromagnetic Field Stimulation Promotes Anti-cell Proliferative Activity in Doxorubicin-treated Mouse Osteosarcoma Cells

“…The possibility of PEMF affecting the intracellular microenvironment of LM8 cells has been previously raised (10,12); thus, we evaluated whether PEMF would accelerate the pharmacological activity of doxorubicin. In this study, the doxorubicin-treated cells immediately after PEMF stimulation for 1 h did not change regarding cell proliferation.…”

P-selectin expression in a colon tumor model exposed by sinusoidal electromagnetic fields

“…Zhang et al (24) have shown no effects of pulsed EMF on the expression of another CAM, integrin, in osteosarcoma cell lines. Further studies should be performed to verify the findings of this study, including new methods for P-selectin detection in vivo and in vitro.…”

Quasi-optical Systems Based on Periodic Structures

“…The spectra Ω nκ of the problem (62), (CS1) - (CS4), and also of the equivalent problem (66) for the dielectric layer with the induced piecewise continuous permittivity at the frequencies nκ of excitation and generation, consist of not more than countable sets of isolated points, without accumulation points in the finite part of each of the surfaces H nκ , n = 1, 2, 3. The resolvents of the spectral problems at these points are poles of finite order.…”

“…Although extremely low electromagnetic fields have been shown to exert beneficial effecets on cartilage tissue (65,66), Lin and Lin investigated the effect of pulsed EMF exposure on osteoblast cells, associated with decreased proliferation and mineralization (67). Okudan, Suslu and co-workers reported the influences of 50 Hz and 0 Hz (static) electric fields (EF), on intact rat bones, as evaluated by dual energy X-ray absorbtion (DEXA) measurements on bone content and density when the animals were continuously exposed in utero and neonatally to EFs.…”

“…The graphs of the eigen-fields of type H 0,0,4 are labeled by #1, the graphs of the eigen-fields of type H 0,0,10 by #2. The numerical results presented in this paper were obtained using an approach based on the description of the wave scattering and generation processes in a non-linear, cubically polarisable layer by a system of non-linear integral equations (49), and of the corresponding spectral problems by the non-trivial solutions of the integral equations (66). We have considered an excitation of the non-linear layer defined by the condition (71).…”