Nobiletin induces brown adipocyte-like phenotype and ameliorates stress in 3T3-L1 adipocytes

Lone, Jameel; Parray, Hilal Ahmad; Yun, Jong Won

doi:10.1016/j.biochi.2017.11.021

Cited by 47 publications

(38 citation statements)

References 44 publications

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“…Furthermore, C. tumida contains polymethoxylated flavones, such as nobiletin and tangeretin (26). It was suggested that nobiletin induces the activation of HIB1B cells, a brown adipocyte cell line derived from a mouse brown fat tumor and mitochondrial biogenesis of 3T3-L1 white adipocytes (27). In addition, chronic nobiletin suppressed body fat and plasma TG in an obese mice model by suppressing PPARg, fatty acid synthase, and sterol regulatory element-binding protein-1c in the liver (28).…”

Section: Discussionmentioning

confidence: 99%

Dietary Intake of Immature Citrus tumida Hort. ex Tanaka Peels Suppressed Body Weight Gain and Fat Accumulation in a Mouse Model of Acute Obesity

Satô

Goto

Inoue

et al. 2019

Journal of Nutritional Science and Vitaminology

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Citrus fruits have several potential benefits for maintaining our health. In this study, we investigated the anti-obesity effects of immature Citrus tumida hort. ex Tanaka (C. tumida) peels using an acute obesity mice model. C57BL/6J male mice were divided into 2 groups; HFD-LL subjected to a high fat diet (HFD) and constant light exposure (LL), and HFDC-LL subjected to a HFD containing immature peel powder of C. tumida (5% w/w) and LL. Dietary ingestion of immature C. tumida peels significantly suppressed body weight gain following decreased epidydimal, perirenal, and subcutaneous fat weights. Blood levels of triglyceride and total cholesterol in the HFDC-LL were significantly lower than those in the HFD-LL group; however, there was no significant difference in food or calorie intake between the 2 groups. These results suggested that immature C. tumida peels have a beneficial effect on the prevention of obesity and metabolic syndrome via its biochemical activities of lipid metabolism.

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Section: Discussionmentioning

confidence: 99%

Dietary Intake of Immature Citrus tumida Hort. ex Tanaka Peels Suppressed Body Weight Gain and Fat Accumulation in a Mouse Model of Acute Obesity

Satô

Goto

Inoue

et al. 2019

Journal of Nutritional Science and Vitaminology

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“…However, there has been no study focused on the effect of nicotine on beige adipocytes in the 3T3-L1 cells. Related research has confirmed the ability of the 3T3-L1 cell line to express beige-related genes [11]. The 3T3-L1 cell line was chosen in our study to explore the effects of nicotine on beige adipocytes at different differentiation stages.…”

Section: Introductionmentioning

confidence: 98%

Nicotine induces a dual effect on the beige-like phenotype in adipocytes

Chen

Xiang

Zhang

et al. 2019

ARCH BIOL SCI BELGRA

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Nicotine, the main component of cigarette smoke, affects white/brown adipocytes. Few studies have concentrated on beige adipocytes. In this study, 3T3-L1 cells were differentiated in the presence of nicotine (25, 50 and 100 µmol/L) during early differentiation and maintenance stages. Cell viability and the state of lipid droplets were assessed by the MTT assay and Oil Red O, respectively, and the expression of beige-related genes and proteins was examined by RT-qPCR, Western blotting and flow cytometry. Nicotine did not alter adipocyte differentiation; however, it increased the expression of peroxisome proliferator-activated receptor gamma (PPARγ) protein during early differentiation and maintenance. Nicotine treatment during early differentiation downregulated gene and protein expression of PPARγ coactivator 1-alpha (PGC-1α), uncoupling protein 1 (UCP1) and cluster of differentiation 137 (CD137), and gene expression of Cbp/p300 interacting transactivator with Glu/ Asp rich carboxy-terminal domain 1 (Cited1), transmembrane protein 26 (Tmem26), and short stature homeobox 2 (Shox2). Nicotine treatment during the maintenance stage upregulated these beige-related genes/proteins. Nicotine treatment of immature adipocytes damaged beige function through a decrease in PGC-1α/UCP1 expression, but nicotine treatment of mature adipocytes or both immature and mature cells enhanced beige functioning. Nicotine induced beige-like phenotype dysfunction in 3T3-L1 adipocytes. This process may affect thermogenesis in adipose tissue and cause a dysfunction in fat metabolism.

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“…19 However, nobiletin (NOB), a polymethoxyflavone abundantly in oranges peel, has been reported to exhibit several biological activities with simple structural and small molecular size. 20,21 Previous studies have shown that nobiletin has an anti-tumor promoting influence on diverse kinds of cancer cells and inhibits inflammatory pathways. 22,23 One of the molecular mechanisms could be regulation of RANKL-induced osteoclastogenesis.…”

Section: Introductionmentioning

confidence: 99%

Nobiletin-loaded micelles reduce ovariectomy-induced bone loss by suppressing osteoclastogenesis

Wang¹,

Xie²,

Ai³

et al. 2019

IJN

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Background: Nobiletin (NOB), a polymethoxy flavonoid, possesses anti-cancer and antiinflammatory activities, has been reported that it played role in anti-osteoporosis treatment. However, previous research did not focus on practical use due to lack of hydrophilicity and cytotoxicity at high concentrations. The aim of this study was to develop a therapeutic formulation for osteoporosis based on the utilization of NOB. Methods: In this study, NOB-loaded poly(ethylene glycol)-block-poly(e-caprolactone) (NOB-PEG-PCL) was prepared by dialysis method. The effects on osteoclasts and antiosteoporosis functions were investigated in a RANKL-induced cell model and ovariectomized (OVX) mice. Results: Dynamic light scattering and transmission electron microscopy examination results revealed that the NOB-PEG-PCL had a round shape, with a mean diameter around 124 nm. The encapsulation efficiency and drug loading were 76.34±3.25% and 7.60±0.48%, respectively. The in vitro release of NOB from NOB-PEG-PCL showed a remarkably sustained releasing characteristic and could be retained at least 48 hrs in pH 7.4 PBS. Anti-osteoclasts effects demonstrated that the NOB-PEG-PCL significantly inhibited the formation of tartrateresistant acid phosphatase (TRAP)-positive multinuclear cells stimulated by RANKL. Furthermore, the NOB-PEG-PCL did not produce cytotoxicity on bone marrow-derived macrophages (BMMs). The mRNA expressions of genetic markers of osteoclasts including TRAP and cathepsin K were significantly decreased in the presence of NOB-PEG-PCL. In addition, the NOB-PEG-PCL inhibited OC differentiation of BMMs through RANKL-induced MAPK signal pathway. After administration of the NOB-PEG-PCL, NOB-PEG-PCL prevented bone loss and improved bone density in OVX mice. These findings suggest that NOB-PEG-PCL might have great potential in the treatment of osteoporosis. Conclusion: The results suggested that NOB-PEG-PCL micelles could effectively prevent NOB fast release from micelles and extend circulation time. The NOB-PEG-PCL delivery system may be a promising way to prevent and treat osteoporosis.

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Nobiletin induces brown adipocyte-like phenotype and ameliorates stress in 3T3-L1 adipocytes

Cited by 47 publications

References 44 publications

Dietary Intake of Immature <i>Citrus tumida</i> Hort. ex Tanaka Peels Suppressed Body Weight Gain and Fat Accumulation in a Mouse Model of Acute Obesity

Dietary Intake of Immature <i>Citrus tumida</i> Hort. ex Tanaka Peels Suppressed Body Weight Gain and Fat Accumulation in a Mouse Model of Acute Obesity

Nicotine induces a dual effect on the beige-like phenotype in adipocytes

<p>Nobiletin-loaded micelles reduce ovariectomy-induced bone loss by suppressing osteoclastogenesis</p>

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