Nobiletin Inhibits Expression of Inflammatory Mediators and Regulates JNK/ERK/p38 MAPK and PI3K/Akt Pathways in Human Osteoarthritic Chondrocytes

Liu, Jianwei; Chong-wei, Hao; Wang, Zhaohui; Jin, Dan

doi:10.4314/tjpr.v15i3.15

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…In comparison with that of the blank group, the mRNA and protein expressions of IL‐1β, TNF‐α, p38, ERK, and JNK were all downregulated in the miR‐24 mimics group, while an opposite trend was shown to occur in the miR‐24 inhibitors group. Liu et al demonstrated that IL‐1β is a major catabolic factor in association with OA and IL‐1β stimulation could cause enhancement in the expressions of JNK, ERK, and p38. Increasing and piling up evidence suggested that OA is associated with the local inflammation and low‐grade system and recently it has been demonstrated that high expressions of IL‐1β, IL‐6, and TNF‐α were also found in patients diagnosed with OA .…”

Section: Discussionmentioning

confidence: 99%

Retracted: Effects of microRNA‐24 targeting C‐myc on apoptosis, proliferation, and cytokine expressions in chondrocytes of rats with osteoarthritis via MAPK signaling pathway

Liu

Zhang

et al. 2017

J of Cellular Biochemistry

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To investigate whether microRNA-24 (miR-24) targeting C-myc affects chondrocytes of rats with osteoarthritis (OA) via the MAPK signaling pathway. Thirty rats were assigned as a sham group and an OA group (established as OA rat models by cutting the anterior cruciate ligaments and removing 1/3 medial meniscus). TUNEL staining and immunohistochemistry were conducted for cell apoptosis index (AI) and positive expression rate of C-myc protein. Enzyme-linked immuno sorbent assay (ELISA) was carried out for serum level of IL-1β and TNF-α. Primary chondrocytes were assigned into the blank, negative control (NC), miR-24 mimics, miR-24 inhibitors, siRNA-C-myc, and miR-24 inhibitors+siRNA-C-myc groups. The expressions of miR-24, C-myc, p38, ERK, JNK, IL-1β, and TNF-α in tissues and cells were detected using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and Western blotting. CCK8 assay and flow cytometry were performed for cell proliferation and apoptosis. The OA group showed higher IL-1β, TNF-α, AI, and C-myc than the sham group. C-myc is a target gene of miR-24. Compared with the blank group, the miR-24 mimics and siRNA-C-myc groups showed reduced expression of C-myc, IL-1β, TNF-α, p38, p-p38, ERK, p-ERK, JNK, and p-JNK, apoptosis rate yet increased cell proliferation; however, the miR-24 inhibitors group exhibited an opposite trend. The miR-24 inhibitors+siRNA-C-myc group presented a same tendency compared to the siRNA-C-myc group. Upregulated miR-24 downregulates C-myc could suppress apoptosis and promote proliferation of chondrocytes to prevent the occurrence and subsequent progression of OA via inactivating the MAPK signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Retracted: Effects of microRNA‐24 targeting C‐myc on apoptosis, proliferation, and cytokine expressions in chondrocytes of rats with osteoarthritis via MAPK signaling pathway

Liu

Zhang

et al. 2017

J of Cellular Biochemistry

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“…However, the side-effects need to be improved. The main components of BWBDS such as amygdalin, cryptochlorogenic acid, nobiletin have been reported to have anti-inflammatory potential in inhibiting IL-6, IL-1 β , TNF- α and relate to signal pathways like phosphatidyl-inositol 3-kinase/serine-threonine kinase, mitogen-activated protein kinase 33 , 34 , 35 , 36 , 37 , 38 . Recent studies showed that administration of Ginseng have been shown to increase the abundance of Lactobacillus 39 , 40 .…”

Section: Discussionmentioning

confidence: 99%

Herbal formula BaWeiBaiDuSan alleviates polymicrobial sepsis-induced liver injury via increasing the gut microbiota Lactobacillus johnsonii and regulating macrophage anti-inflammatory activity in mice

Fan

Mai²,

Zuo³

et al. 2023

Acta Pharmaceutica Sinica B

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“…Nobiletin was shown to block pro-MMP-9 production and reduce its mRNA and exhibit reduced PGE2 caused by IL-1β in the synovial cells [34]. Nobiletin significantly improved the viability of human osteoarthritic chondrocytes stimulated with IL-1β and remarkably reduced the levels of nitrite, IL-6, and PGE2 through the inhibition of the JNK/ERK/p38 MAPK and PI3K/Akt pathways [35]. Combining our findings and this research indicated SE-mediated chondrocyte protection correlated with the downregulation of inflammation.…”

Section: Discussionmentioning

confidence: 99%

Dietary Supplements of Shiikuwasha Extract Attenuates Osteoarthritis Progression in Meniscal/ligamentous Injury and Obese Rats

2019

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Osteoarthritis (OA), also called degenerative joint disease, is characterized by joint cartilage loss and is strongly linked to obesity. Medicine to alleviate pain is currently the only treatment. Shiikuwasha extract (SE) has been reported to possess valuable bioactive substances exhibiting anti-inflammatory, antiobesity, and anticancer effects. Research is limited to the use of SE in the treatment of OA and obesity. We performed both anterior cruciate ligament transections and medial meniscectomies to induce OA on Sprague–Dawley rats after 11 weeks of a high fat diet followed by 9 weeks of oral SE administration (300, 600, and 1500 mg/kg). This study showed that SE treatment could reduce weight gain and joint pain. Additionally, SE significantly decreased triglycerides and total cholesterol in plasma of the S1500 group but increased high-density lipoprotein cholesterol in the plasma of the S600 group. Meanwhile, plasma levels of tumor necrosis factor alpha (TNF-α) was significantly reduced in the S1500 groups. Histopathological findings confirmed administration of SE attenuated cartilage degeneration. Immunohistochemistry examination demonstrated that caspase 3 and phospho-Janus kinase 2 (p-JAK2) expression levels on chondrocytes were downregulated by SE treatment. Our findings demonstrate that SE can alleviate OA progression by improving obesity.

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Nobiletin Inhibits Expression of Inflammatory Mediators and Regulates JNK/ERK/p38 MAPK and PI3K/Akt Pathways in Human Osteoarthritic Chondrocytes

Cited by 9 publications

References 27 publications

Retracted: Effects of microRNA‐24 targeting C‐myc on apoptosis, proliferation, and cytokine expressions in chondrocytes of rats with osteoarthritis via MAPK signaling pathway

Retracted: Effects of microRNA‐24 targeting C‐myc on apoptosis, proliferation, and cytokine expressions in chondrocytes of rats with osteoarthritis via MAPK signaling pathway

Herbal formula BaWeiBaiDuSan alleviates polymicrobial sepsis-induced liver injury via increasing the gut microbiota Lactobacillus johnsonii and regulating macrophage anti-inflammatory activity in mice

Dietary Supplements of Shiikuwasha Extract Attenuates Osteoarthritis Progression in Meniscal/ligamentous Injury and Obese Rats

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