We report the first direct measurement of the helium isotope ratio, 3 He/ 4 He, outside of the Local Interstellar Cloud, as part of science verification observations with the upgraded CRyogenic InfraRed Echelle Spectrograph (CRIRES). Our determination of 3 He/ 4 He is based on metastable He i* absorption along the line-of-sight towards Θ 2 A Ori in the Orion Nebula. We measure a value 3 He/ 4 He = (1.77 ± 0.13) × 10 −4 , which is just ∼ 40 per cent above the primordial relative abundance of these isotopes, assuming the Standard Model of particle physics and cosmology, ( 3 He/ 4 He) p = (1.257 ± 0.017) × 10 −4 . We calculate a suite of galactic chemical evolution simulations to study the Galactic build up of these isotopes, using the yields from Limongi & Chieffi (2018) for stars in the mass range M = 8 − 100 M and Lagarde et al. (2011, 2012) for M = 0.8 − 8 M . We find that these simulations simultaneously reproduce the Orion and protosolar 3 He/ 4 He values if the calculations are initialized with a primordial ratio ( 3 He/ 4 He) p = (1.043 ± 0.089) × 10 −4 . Even though the quoted error does not include the model uncertainty, this determination agrees with the Standard Model value to within ∼ 2σ. We also use the present-day Galactic abundance of deuterium (D/H), helium (He/H), and 3 He/ 4 He to infer an empirical limit on the primordial 3 He abundance, ( 3 He/H) p ≤ (1.09 ± 0.18) × 10 −5 , which also agrees with the Standard Model value. We point out that it is becoming increasingly difficult to explain the discrepant primordial 7 Li/H abundance with non-standard physics, without breaking the remarkable simultaneous agreement of three primordial element ratios (D/H, 4 He/H, and 3 He/ 4 He) with the Standard Model values. * Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere under ESO programme(s) 107. 22U1.001, 194.C-0833.