Nocardia puris sp. nov.

Yassin, A. F.; Sträubler, Bettina; Schümann, Peter; Schaal, K. P.

doi:10.1099/ijs.0.02593-0

Cited by 37 publications

(17 citation statements)

References 35 publications

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“…Since that time, several of the new species are represented by only one strain or a few strains, so their occurrence in humans still has to be assessed (6,8,11,12,17,28).…”

Section: Discussionmentioning

confidence: 99%

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Distribution of Nocardia Species in Clinical Samples and Their Routine Rapid Identification in the Laboratory

Wauters¹,

Avesani²,

Charlier³

et al. 2005

J Clin Microbiol

100

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Eighty-six. A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, ␥-glutamyl aminopeptidase, ␣-mannosidase, and ␣-glucosidase were found to be highly discriminating and could be used to develop an identification scheme.Nocardia species are isolated with an increased frequency from clinical specimens, especially in specimens from immunocompromised patients (19). The taxonomy of the genus has dramatically been revised during the last decade, and at least 30 valid species have been reported, besides a number of unnamed genomospecies (18). Not all of them have been found in humans, and Nocardia asteroides, previously most frequently isolated from clinical specimens, has proved to be heterogeneous and has been divided into several species (18). More recently, additional species of human origin have been described (6, 8, 9, 11, 12, 17, 28).The routine identification of Nocardia strains at the species level is difficult in the laboratory. This, and the nomenclature changes, may explain that species distribution in clinical isolates has been poorly documented up to now, and even recent surveys still report the "N. asteroides complex" as the most frequent Nocardia species isolated in humans (7,10,16,19). Identification studies have not been systematically carried out since the several recent taxonomic changes.The aim of this study was to assess the species distribution of a large number of Nocardia isolates and to propose simple and rapid identification tests that may be helpful to identify the species most commonly encountered in clinical material. MATERIALS AND METHODSBacterial strains. Eighty-six Nocardia strains isolated from clinical specimens in Belgium were collected for the study. Most strains were isolated during the past decade, but a few were received before 1990. They were isolated by several laboratories in different parts of the country. All the Nocardia isolates were included in the study to avoid any bias in the selection of the strains. Only one strain per patient was considered. The clinical origins of the isolates were as follows: 36 strains were isolated from the respiratory tract, 18 from pyogenic lesions and wounds, 8 from blood, 4 from brain abscesses, and 1 from cerebral fluid. Nineteen were of unknown origin.The type strains of the most relevant species were included for phenotypic comparison as well as reference strains of some less common species. Nocardia species and strains are listed in Table 1.Sequencing of the 16S rRNA gene. The full-length 16S rRNA gene sequences (Ϯ1,400 nucleotides) of all strains were determined as described previously (24), and sequences were compared to those of the type strains deposited in the GenBank database.Cellular fatty acids were analyzed by gas-liquid chromatography as outlined elsewhere (22).Phenotypic characterization. Strains were cultured on tryptic soy blood agar plates at 35°C. They were examined for partial acid-fastness, presenc...

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“…Since that time, several of the new species are represented by only one strain or a few strains, so their occurrence in humans still has to be assessed (6,8,11,12,17,28).…”

Section: Discussionmentioning

confidence: 99%

“…Not all of them have been found in humans, and Nocardia asteroides, previously most frequently isolated from clinical specimens, has proved to be heterogeneous and has been divided into several species (18). More recently, additional species of human origin have been described (6,8,9,11,12,17,28).…”

mentioning

confidence: 99%

Distribution of Nocardia Species in Clinical Samples and Their Routine Rapid Identification in the Laboratory

Wauters¹,

Avesani²,

Charlier³

et al. 2005

J Clin Microbiol

100

View full text Add to dashboard Cite

show abstract

“…According to Yassin et al (2003), numerous members of the genus Nocardia have distinctive phenotypic characteristics, which justify their classification as separate species, but they show limited divergence within their 16S-rDNA gene sequences. An example of this can be seen in the 99.3% 16S-rDNA similarity of Nocardia veterana and Nocardia africana (Yassin et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Nocardia gamkensis sp. nov.

Roes

Meyers

2006

Antonie van Leeuwenhoek

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A novel actinomycete, strain CZH20(T), was isolated from a soil sample taken from the banks of the Gamka River in the Swartberg Nature Reserve, Western Cape Province, South Africa. Strain CZH20(T) was identified as a member of the genus Nocardia by a polyphasic approach. Strain CZH20(T) could be differentiated from other members of the genus Nocardia on the basis of physiology and 16S-rRNA gene sequence analysis. It exhibited weak antibiosis against Mycobacterium aurum A+. Organic solvent extracts of the culture filtrate and mycelial mass of CZH20(T) exhibited moderate antibiotic activity against Mycobacterium smegmatis LR222 and Mycobacterium tuberculosis H37Rv. The name Nocardia gamkensis is proposed, with the type strain CZH20(T) (=DSM 44956(T) =NRRL B-24450(T)).

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“…Conville et al (6), for example, recommend that care should be used in the interpretation of results of restriction enzyme profiles of the hsp gene alone, as several species, N. africana, N. nova, and N. veterana, have the same enzymatic profile as well as the same antibiotic susceptibility profile. In addition, difficulties have been encountered in the identification of Nocardia isolates solely by comparison of 16S rRNA gene sequences (6,7,21,32). Previously, strains having Ͻ3% differences between their 16S rRNA gene sequences were considered the same species (24).…”

Section: Discussionmentioning

confidence: 99%

Rapid Identification of Nocardia farcinica Clinical Isolates by a PCR Assay Targeting a 314-Base-Pair Species-Specific DNA Fragment

et al. 2004

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Nocardia farcinica is the most clinically significant species within the Nocardia asteroides complex. Differentiation of N. farcinica from other members of N. asteroides complex is important because this species characteristically demonstrates resistance to several extended-spectrum antimicrobial agents. Traditional phenotypic characterization of this species is time-and labor-intensive and often leads to misidentification in the clinical microbiology laboratory. We previously observed a 409-bp product for all strains of N. farcinica by using randomly amplified polymorphic DNA analysis with the primer DKU49. In this investigation, the 409-bp fragment was sequenced and then used to design a specific primer pair, Nf1 (16-mer) and Nf2 (16-mer), complementary to the 409-bp fragment. PCR amplification of genomic DNA from 28 N. farcinica isolates with Nf1 and Nf2 generated a single intense 314-bp fragment. The specificity of the assay with these primers was verified, since there were no PCR amplification products observed from heterologous nocardial species (n ‫؍‬ 59) or other related bacterial genera (n ‫؍‬ 41). Restriction enzyme digestion using CfoI and direct sequencing of the 314-bp fragment further confirmed the specificity of the assay for N. farcinica. This highly sensitive and specific PCR assay provides a rapid (within 1 day of obtaining DNA) method for identification of this medically important emerging pathogen. Rapid diagnosis of N. farcinica infection may allow for earlier initiation of effective therapy, thus improving patient outcome.

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Nocardia puris sp. nov.

Cited by 37 publications

References 35 publications

Distribution of Nocardia Species in Clinical Samples and Their Routine Rapid Identification in the Laboratory

Distribution of Nocardia Species in Clinical Samples and Their Routine Rapid Identification in the Laboratory

Nocardia gamkensis sp. nov.

Rapid Identification of Nocardia farcinica Clinical Isolates by a PCR Assay Targeting a 314-Base-Pair Species-Specific DNA Fragment

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