Nocardioides dongkuii sp. nov. and Nocardioides lijunqiniae sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) and leaves of dandelion (Taraxacum officinale), respectively, on the Qinghai–Tibet Plateau

Abstract

In the present study, four bacterial strains, two (S-713 T and 406) isolated from faecal samples of Tibetan antelopes and the other two (S-531 T and 1598) from leaves of dandelion collected on the Qinghai–Tibet Plateau of PR China, were analysed using a polyphasic approach. All four isolates were aerobic, rod-shaped, non-motile, oxidase-negative, Gram-stain-positive and catalase-positive. According to four phylogenetic trees, strain pairs S-713 T /406 and S-531 T /1598 form two independent branches belonging to the genus Nocardioides , and are closest to Nocardioides lianchengensis , Nocardioides dokdonensis , Nocardioides salarius , Nocardioides marinisabuli , Nocardioides psychrotolerans and Nocardioides szechwanensis . Although sharing MK8-(H 4 ) as their major isoprenoid quinone, strains S-713 T and S-531 T contained C 18 : 1 ω 9 c (24.64 and 16.34 %) and iso-C 16 : 0 (9.74 and 29.38 %), respectively, as their main fatty acids, with remarkable differences in their biochemical profiles but only slight ones in their optimal growth conditions. The chromosomes of strains S-713 T and S-531 T were 4 207 844 bp (G+C content, 73.0 mol%) and 4 809 817 bp (G+C content, 72.5 mol%), respectively. Collectively, the two strain pairs represent two separate novel species of the genus Nocardioides , for which the names Nocardioides dongkuii sp. nov. and Nocardioides lijunqiniae sp. nov. are proposed, with S-713 T (=JCM 33698 T =CGMCC 4.7660 T ) and S-531 T (=JCM 33468 T =CGMCC 4.7659 T ) as the respective type strains.

Identification of Nocardioides marmotae sp. nov. and Nocardioides faecalis sp. nov., two new members of the genus Nocardioides

“…MK-8(H 4 ) was the major respiratory quinone detected in strains zg-579 T and zg-536 T , with contents of 68.1 and 88.5 %, respectively. In addition, the two new type strains contained ll -diaminopimelic acid as the diagnostic diamino acid, which was in line with other known species within the genus Nocardioides [20, 27, 28, 50–54]. Strains zg-579 T and zg-536 T shared galactose and ribose in their cell wall, whereas glucose was also found in zg-579 T ; however, data about the cell-wall sugar components of their respective neighbours were not available.…”

“…S3b). Remarkably, the presence of phosphatidylcholine in strain zg-579 T enabled distinguishing it from its three neighbours [27,[50][51][52]. The identifiable polar lipids in zg-536 T were similar to those detected in N. nitrophenolicus JCM 10703 T [20], but were unknown for N. caeni DSM 23134 T and N. daeguensis 2C1-5 T .…”

“…S3b). Remarkably, the presence of phosphatidylcholine in strain zg-579 T enabled distinguishing it from its three neighbours [27, 50–52]. The identifiable polar lipids in zg-536 T were similar to those detected in N.…”

“…), plants ( Lamiophlomis rotate , Taraxacum officinale , etc. ), and soil, nine novel species within this genus had been identified [11–13, 25, 27, 28]. In this study, a polyphasic taxonomic characterization of another two novel species within this genus was conducted, which were collected from the faeces of Marmota himalayana .…”

Nocardioides mangrovi sp. nov., a novel endophytic actinobacterium isolated from root of Kandelia candel

“…Among these, the recently reported Nocardioides were separated from a variety of environments, such as Acrostichum aureum, Tagetes patula, soil of rhizosphere, tibetan antelope, dandelion, sewage sludge, cave, etc. [2][3][4][5][6][7]. Members of the genus Nocardioides are characterized as being Gram-positive and aerobic with a high DNA G+C content (68.7-74.9 mol%).…”