Nocardioides dongxiaopingii sp. nov., isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau

Zhang, Sihui; Wang, Xiaoxia; Yang, Jing; Lu, Shan; Lai, Xin‐He; Jin, Dong; Huang, Yu‐Yuan; Zhu, Wentao; Li, Junqin; Pu, Ji; Huang, Ying; Dong, Kui; Gui, Zhilun; Lei, Wenjing; Wang, Suping; Xu, Jianguo

doi:10.1099/ijsem.0.004161

Cited by 17 publications

(13 citation statements)

References 39 publications

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“…At the time of writing, this genus encompasses 122 validly published species with correct names (www.bacterio.net/genus/nocardioides). Nocardioides strains were isolated from various habitats including soil [2, 3], mud [4], marine sediment [5], glacier [6], caves [7], fresh water [8, 9], wastewater [10], faeces of wild animal [11–13], plant leaves [14] and roots [15]. Members of Nocardioides are Gram-stain-positive, rod- or coccus-shaped, non-acid-fast and either motile or non-motile with typical features including a high DNA G+C content (64.0–74.9 mol%), MK-8(H 4 ) as the predominant respiratory quinone, iso-C 16 : 0 as the major fatty acid and ll -2,6-diaminopimelic acid as the cell-wall peptidoglycan [16].…”

Section: Introductionmentioning

confidence: 99%

“…It provides a hugely varied habitat for a myriad of wild animals and plants, and a highly elevated region with low partial pressure of oxygen. To date, several novel bacterial species of the genus Nocardioides have been isolated from animal faecal or plant leaf samples collected from this plateau, such as Nocardioides houyundeii from Tibetan antelope [13], Nocardioides jishulii from Tibetan gazelle [11], Nocardioides yefusunii from Equus kiang (Tibetan wild ass) [12] and Nocardioides dongxiaopingii from the leaves of Lamiophlomis rotata [14].…”

Section: Introductionmentioning

confidence: 99%

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Nocardioides dongkuii sp. nov. and Nocardioides lijunqiniae sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) and leaves of dandelion (Taraxacum officinale), respectively, on the Qinghai–Tibet Plateau

Cheng

Jiao

Zhang

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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In the present study, four bacterial strains, two (S-713 T and 406) isolated from faecal samples of Tibetan antelopes and the other two (S-531 T and 1598) from leaves of dandelion collected on the Qinghai–Tibet Plateau of PR China, were analysed using a polyphasic approach. All four isolates were aerobic, rod-shaped, non-motile, oxidase-negative, Gram-stain-positive and catalase-positive. According to four phylogenetic trees, strain pairs S-713 T /406 and S-531 T /1598 form two independent branches belonging to the genus Nocardioides , and are closest to Nocardioides lianchengensis , Nocardioides dokdonensis , Nocardioides salarius , Nocardioides marinisabuli , Nocardioides psychrotolerans and Nocardioides szechwanensis . Although sharing MK8-(H 4 ) as their major isoprenoid quinone, strains S-713 T and S-531 T contained C 18 : 1 ω 9 c (24.64 and 16.34 %) and iso-C 16 : 0 (9.74 and 29.38 %), respectively, as their main fatty acids, with remarkable differences in their biochemical profiles but only slight ones in their optimal growth conditions. The chromosomes of strains S-713 T and S-531 T were 4 207 844 bp (G+C content, 73.0 mol%) and 4 809 817 bp (G+C content, 72.5 mol%), respectively. Collectively, the two strain pairs represent two separate novel species of the genus Nocardioides , for which the names Nocardioides dongkuii sp. nov. and Nocardioides lijunqiniae sp. nov. are proposed, with S-713 T (=JCM 33698 T =CGMCC 4.7660 T ) and S-531 T (=JCM 33468 T =CGMCC 4.7659 T ) as the respective type strains.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nocardioides dongkuii sp. nov. and Nocardioides lijunqiniae sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) and leaves of dandelion (Taraxacum officinale), respectively, on the Qinghai–Tibet Plateau

Cheng

Jiao

Zhang

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…The presence of meso -diaminopimelic acid was detected using the method described by Schumann [52] and the release of mycolic acids after acid hydrolysis was measured using TLC [53]. Analysis of whole-cell sugar was carried out using a previously described method [54].…”

Section: Full-textmentioning

confidence: 99%

Corynebacterium lizhenjunii sp. nov., isolated from the respiratory tract of Marmota himalayana, and Corynebacterium qintianiae sp. nov., isolated from the lung tissue of Pseudois nayaur

Zhou

Guo

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Four Gram-stain-positive, non-motile and asporous bacilli (strains ZJ-599T, ZJ-621, MC1420T and MC1482), isolated from animal tissue and environmental samples collected on the Qinghai-Tibet Plateau, PR China, were taxonomically characterized. Based on the results of 16S rRNA gene sequence analyses, the closest relatives of strains ZJ-599T and ZJ-621 were Corynebacterium endometrii LMM-1653T (97.5 %), Corynebacterium phocae M408/89/1T (96.5 %) and Corynebacterium flavescens OJ8T (96.3 %), whereas strains MC1420T and MC1482 were closest to Corynebacterium sanguinis CCUG 58655T (98.9 %), Corynebacterium mycetoides DSM 20632T (98.4 %) and Corynebacterium lipophiloflavum DSM 44291T (97.9 %). The results of rpoB gene sequence similarity analysis indicated that C. phocae M408/89/1T and C. sanguinis CCUG 58655T were closest to strains ZJ-599T/ZJ-621 (83.5 %) and MC1420T/MC1482 (91.8 %), respectively. The two novel type strains shared a similarity of 95.2 % in 16S rRNA and 81.3 % in rpoB gene sequences. The TAP-PCR DNA fingerprint and MALDI-TOF MS spectrum patterns clearly differentiated the novel isolates within and between each pair of strains. Strain ZJ-599T had 21.9–22.4 % digital DNA–DNA hybridization (dDDH) scores with C. endometrii LMM-1653T, C. phocae M408/89/1T and C. flavescens OJ8T, and 72.3–72.9 % of average nucleotide identity (ANI) with them. Similarly, strain MC1420T had 22.9–23.7 % dDDH values with C. sanguinis CCUG 58655T, C. mycetoides DSM 20632T and C. lipophiloflavum DSM 44291T, and 80.4–81.3 % ANI scores with them. Strain ZJ-599T had a 23.1 % dDDH value and 70.5 % ANI score with strain MC1420T, both below the corresponding thresholds for species delineation. Strains ZJ-599T and MC1420T both contain mycolic acids and have MK-8(H2) and MK-9(H2) as the predominant respiratory quinones, meso-diaminopimelic acid as the diagnostic diamino acid, and C18 : 1 ω9c as the main fatty acid. C17 : 1 ω8c and C15 : 1 ω8c were predominant in strain ZJ-599T in contrast to C17 : 1 ω7c being predominant in strain MC1420T. The main polar lipids in strain ZJ-599T were diphosphatidylglycerol, phosphatidylinositol and one unidentified glycolipid, while strain MC1420T had diphosphatidylglycerol, phosphatidylglycerol and one unidentified lipid as the major components. Since the two pairs of novel strains (ZJ-599T/ZJ-621, MC1420T/MC1482) distinctly differ from each other and from their nearest relatives, two novel species of the genus Corynebacterium are proposed, namely Corynebacterium lizhenjunii (type strain ZJ-599T=GDMCC 1.1779T=JCM 34341T) and Corynebacterium qintianiae (type strain MC1420T=GDMCC 1.1783T=JCM 34340T), respectively.

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“…Whole-cell sugar compositions were examined based on the methods described by Schleifer [38]. For detection of the amino acids in the peptidoglycan, the purified cell wall hydrolysates (6M HCl, 120 °C for 15 min) were analysed as previously described [31].…”

Section: Chemotaxonomic Analysismentioning

confidence: 99%

“…Oxygen requirement for growth was assessed by culturing cells under aerobic, anaerobic or aerobic plus 5 % CO 2 condition. The temperature, pH and NaCl tolerance range for cell growth were also tested as previously described [31] with BHI broth as the basal medium. Catalase and oxidase activities were determined using bubble production with 3 % (v/v) hydrogen peroxide solution and indophenol blue production with 1 % (w/v) tetramethyl- p -phenylenediamine, respectively.…”

Section: Phenotypic Analysesmentioning

confidence: 99%

Characterization of isolates of members of the genus Actinomyces from Marmota himalayana: description of Actinomyces faecalis sp. nov., Actinomyces respiraculi sp. nov., and Actinomyces trachealis sp. nov.

Zhou

Zhang

Gui

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Six novel strains (ZJ34T, ZJ561, ZJ750T, ZJ1629, zg-993T and zg-987) isolated from faeces and respiratory tracts of Marmota himalayana from the Qinghai–Tibet Plateau of PR China were characterized comprehensively. The results of analyses of the 16S rRNA gene and genome sequences indicated that the six strains represent three novel species of the genus Actinomyces , and are closely related to Actinomyces urogenitalis DSM 15434T (16S rRNA gene sequences similarities, 94.9–98.7 %), Actinomyces weissii CCUG 61299T (95.6–96.6 %), Actinomyces bovis CCTCC AB2010168T (95.7 %) and Actinomyces bowdenii DSM 15435T (95.2–96.4 %), with values of digital DNA–DNA hybridization less than 30.1 % when compared with their closest relatives but higher than 70 % within each pair of novel strains (ZJ34T/ZJ561, ZJ750T/ZJ1629 and zg-993T/zg-987). All the novel strains had C18 : 1 ω9c and C16 : 0 as the two most abundant major fatty acids. MK-9(H4) or MK-8(H4) was the sole or predominant respiratory quinone of strains ZJ34T, ZJ750T and zg-993T and their polar lipid profiles differed, but all had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphatidyl inositol mannoside as major components. ZJ750T shared identical peptidoglycan amino acid profile with ZJ34T (alanine, glutamic acid, lysine and ornithine) and the same whole-cell sugar composition with zg-993T (glucose, rhamnose and ribose). Strain zg-993T contained alanine, aspartic acid, glutamic acid, glycine and lysine in the peptidoglycan, and the only sugar in ZJ34T was ribose. The DNA G+C contents of the novel strains were within the range of 65.8–70.1 mol%. On the basis of the results from the aforementioned analyses, the six novel strains were classified as representing three novel species of genus Actinomyces , for which the names Actinomyces faecalis sp. nov. [type strain ZJ34T (=GDMCC 1.1952T=JCM 34355T)], Actinomyces respiraculi sp. nov. [type strain ZJ750T (=GDMCC 1.1950T=JCM 34356T)] and Actinomyces trachealis sp. nov. [type strain zg-993T (=GDMCC 1.1956T=JCM 34357T)] were proposed, respectively.

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Nocardioides dongxiaopingii sp. nov., isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau

Cited by 17 publications

References 39 publications

Nocardioides dongkuii sp. nov. and Nocardioides lijunqiniae sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) and leaves of dandelion (Taraxacum officinale), respectively, on the Qinghai–Tibet Plateau

Nocardioides dongkuii sp. nov. and Nocardioides lijunqiniae sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) and leaves of dandelion (Taraxacum officinale), respectively, on the Qinghai–Tibet Plateau

Corynebacterium lizhenjunii sp. nov., isolated from the respiratory tract of Marmota himalayana, and Corynebacterium qintianiae sp. nov., isolated from the lung tissue of Pseudois nayaur

Characterization of isolates of members of the genus Actinomyces from Marmota himalayana: description of Actinomyces faecalis sp. nov., Actinomyces respiraculi sp. nov., and Actinomyces trachealis sp. nov.

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