Node and midline defects are associated with left-right development in<i>Delta1</i>mutant embryos

Przemeck, Gerhard K. H.; Heinzmann, Ulrich; Beckers, Johannes; Angelis, Martin Hrabé de

doi:10.1242/dev.00176

Cited by 94 publications

(91 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1A with 1B). A lack of a precise correlation between the expression of nodal and pitx2 in the LPM was already described in the context of delta-like 1 (Dll1) mouse mutants (Krebs et al, 2003;Przemeck et al, 2003;Raya et al, 2003). Altogether, these results show that Notch signalling regulates pitx2 expression in a Nodal/Spaw independent manner.…”

Section: Deltad Is the Notch Ligand That Defines Laterality In Zebrafishmentioning

confidence: 63%

“…However, there are differences in the mode of action of this molecular signalling pathway between mammals and fish. In the mouse, the analysis of Notch pathway mutants reveals that nodal induction in the murine node is impaired (Krebs et al, 2003;Przemeck et al, 2003;Raya et al, 2003;Takeuchi et al, 2007), whereas the expression of the secreted Nodal antagonist cerl2 does not seem to be affected (Krebs et al, 2003;Przemeck et al, 2003;Raya et al, 2003;Takeuchi et al, 2007). By contrast, the expression of spaw is normal in zebrafish embryos treated with DAPT (a Notch signalling inhibitor) but the expression of the secreted Nodal antagonist charon is severely affected in the KV (Gourronc et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Notch signalling has been implicated in LR patterning in vertebrates, largely through the activation of Nodal expression in the murine node (Krebs et al, 2003;Przemeck et al, 2003;Raya et al, 2003;Takeuchi et al, 2007). In zebrafish, Notch signalling seems to activate charon expression in KV (Gourronc et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Notch signalling regulates left-right asymmetry through ciliary length control

et al. 2010

View full text Add to dashboard Cite

SUMMARYThe importance of cilia in embryonic development and adult physiology is emphasized by human ciliopathies. Despite its relevance, molecular signalling pathways behind cilia formation are poorly understood. We show that Notch signalling is a key pathway for cilia length control. In deltaD zebrafish mutants, cilia length is reduced in Kupffer´s vesicle and can be rescued by the ciliogenic factor foxj1a. Conversely, cilia length increases when Notch signalling is hyperactivated. Short cilia found in deltaD mutants reduce the fluid flow velocity inside Kupffer´s vesicle, thus compromising the asymmetric expression of the flow sensor charon. Notch signalling brings together ciliary length control and fluid flow hydrodynamics with transcriptional activation of laterality genes. In addition, our deltaD mutant analysis discloses an uncoupling between gut and heart laterality.

show abstract

Section: Deltad Is the Notch Ligand That Defines Laterality In Zebrafishmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Notch signalling regulates left-right asymmetry through ciliary length control

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The primary embryonic organizer, Spemann's organizer in the amphibian embryo, Hensen's node in rabbit and mouse, and the embryonic shield in the teleost fish embryo, from which the dorsal forerunner cells and hence Kupffer's vesicle (KV) are derived, thus sets the stage for the development of the PNC/GRP/KV or its equivalent in other vertebrates, which func-tionally may turn out to be primarily involved in LR axis development. Experimental manipulation of the organizer thus affects all three body axes, while defects in PNC/GRP/KV cause laterality defects without affecting the main body axes (Herrmann and Kispert, 1994;Nonaka et al, 1998;Krebs et al, 2003;Przemeck et al, 2003;Abdelkhalek et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo

Feistel

Blum

2006

Developmental Dynamics

View full text Add to dashboard Cite

Motile monocilia play a pivotal role in left-right axis determination in mouse and zebrafish embryos. Cilia with 9؉0 axonemes localize to the distal indentation of the mouse egg cylinder ("node"), while Kupffer's vesicle cilia in zebrafish show 9؉2 arrangements. Here we studied cilia in a prototype mammalian embryo, the rabbit, which develops via a flat blastodisc. Transcription of ciliary marker genes

show abstract

“…Whether the choice of Hensen's node zone c cells to enter either the floor plate or the notochord differentiation pathway results from intrinsic cell fate decision and/or from extrinsic signals cannot be deduced from the previous data. However, recent results in several animal models are in favor of a balance between notochord and floor plate cells by cell fate decision at the level of the organizer (Appel et al, 1999;Lopez et al, 2003;Przemeck et al, 2003). Notch-Delta signaling is a good candidate for controlling this process.…”

Section: Hensen's Node Cell Fate and Differentiationmentioning

confidence: 99%

Cellular dynamics and molecular control of the development of organizer-derived cells in quail-chick chimeras

Charrier¹,

Catala²,

Lapointe³

et al. 2005

Int. J. Dev. Biol.

View full text Add to dashboard Cite

Malformations affecting the nervous system in humans are numerous and various in etiology. Many are due to genetic deficiencies or mechanical accidents occurring at early stages of development. It is thus of interest to reproduce such human malformations in animal models. The avian embryo is particularly suitable for researching the role of morphogenetic movements and genetic signaling during early neurogenesis. The last ten years of research with Nicole Le Douarin in the Nogent Institut have brought answers to questions formulated by Etienne Wolff at the beginning of his career, by showing that Hensen's node, the avian organizer, is at the source of all the midline cells of the embryo and ensures cell survival, growth and differentiation of neural and mesodermal tissues. KEY WORDS: neurulation, Hensen's node, floor plate, quail-chick chimera, caudal dysplasia ForewordEtienne Wolff, the founder of the Nogent Institute, was a pioneer in teratogenic studies. He was the first to use the chick embryo as an animal model to construct phenocopies of human developmental malformations (Wolff, 1936). During his PhD thesis under the supervision of Paul Ancel in Strasbourg University, he experimentally reproduced cyclopia and symmelia, two dramatic malformations occasionally observed in human embryos, affecting organ bilaterality. He obtained phenocopies of such malformations in bird embryos by performing axial organ destruction using X irradiation at early stages of development (Fig. 1). Sixty years after these pioneer experiments, with Nicole Le Douarin, a former student of Etienne Wolff and his successor at the head of the Nogent Institute, we used the quail-chick cell labeling technique (Le Douarin, 1969) and microsurgical excision to elucidate the role of the avian organizer, Hensen's node, during the development of the central nervous system in the avian embryo. The results that we obtained confirmed and extended Wolff's observations and, like them, could be related to known human malformations. In the present paper we will review several aspects of our recent work, the aim of which was to improve our general understanding of the early development of the vertebrate nervous system. The first stages of neurogenesis: «primary» versus «secondary» neurulationIn birds, as in other chordates, a region of the primitive ectoderm is precociously induced to form the neurectoderm (Stern, 2002 for a review). Neural induction leads to the formation of a prismatic cell layer organized into the so-called neural plate. This plate will undergo a series of subsequent morphogenetic movements called neurulation, giving rise to the neural tube, i.e., the primordium of the central nervous system (CNS). Vertebrate neurulation proceeds according to two different mechanisms regarding the type of cell movements implicated (Catala et al., 1995;Colas and Schoenwolf, 2001 and references therein). In the first, which affects the anterior moiety of the bird embryo, the neural plate bends due to the formation of several hinge points (one me...

show abstract

Node and midline defects are associated with left-right development inDelta1mutant embryos

Cited by 94 publications

References 83 publications

Notch signalling regulates left-right asymmetry through ciliary length control

Notch signalling regulates left-right asymmetry through ciliary length control

Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo

Cellular dynamics and molecular control of the development of organizer-derived cells in quail-chick chimeras

Contact Info

Product

Resources

About