Nomenclature for factors of the <scp>HLA</scp> system, update December 2012

Marsh, Steven G.E.

doi:10.1111/tan.12077

Cited by 7 publications

(1 citation statement)

References 12 publications

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“…HLA low/medium resolution typing for HLA-DRB1 and HLA–DQB1 alleles was performed using PCR-sequence specific oligonucleotide (SSO) Luminex LABTYPE ® SSO kits designed to recognize all the broad specificities based on the sequence databases from IMGT/HLA Database (database version 3.17.0) (http://www.ebi.ac.uk/ipd/imgt/hla/probe.html) [28, 29]. …”

Section: Methodsmentioning

confidence: 99%

Protective effect of HLA-DQB1 alleles against alloimmunization in patients with sickle cell disease

et al. 2016

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Background Alloimmunization or the development of alloantibodies to Red Blood Cell (RBC) antigens is considered one of the major complications after RBC transfusions in patients with sickle cell disease (SCD) and can lead to both acute and delayed hemolytic reactions. It has been suggested that polymorphisms in HLA genes, may play a role in alloimmunization. We conducted a retrospective study analyzing the influence of HLA-DRB1 and DQB1 genetic diversity on RBC-alloimmunization. Study design Two-hundred four multi-transfused SCD patients with and without RBC-alloimmunization were typed at low/medium resolution by PCR-SSO, using IMGT-HLA Database. HLA-DRB1 and DQB1 allele frequencies were analyzed using logistic regression models, and global p-value was calculated using multiple logistic regression. Results While only trends towards associations between HLA-DR diversity and alloimmunization were observed, analysis of HLA-DQ showed that HLA-DQ2 (p=0.02), -DQ3 (p=0.02) and -DQ5 (p=0.01) alleles were significantly higher in non-alloimmunized patients, likely behaving as protective alleles. In addition, multiple logistic regression analysis showed both HLA-DQ2/6 (p=0.01) and HLA-DQ5/5 (p=0.03) combinations constitute additional predictor of protective status. Conclusion Our data suggest that particular HLA-DQ alleles influence the clinical course of RBC transfusion in patients with SCD, which could pave the way towards predictive strategies.

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Section: Methodsmentioning

confidence: 99%