Non-aqueous capillary electrophoresis with red light emitting diode absorbance detection for the analysis of basic dyes

Fakhari, Ali Reza; Breadmore, Michael C.; Macka, Mirek; Haddad, Paul R.

doi:10.1016/j.aca.2006.07.066

Cited by 37 publications

(20 citation statements)

References 20 publications

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“…In the area of on-capillary detection including CE, LEDs have been used in miniaturised low-cost detection systems, both photometric [29][30][31][32][33][34][35][36][37][38][39][40] and LED induced fluorimetric (LED-IF) [41][42][43][44][45], with advantages especially for portable CE instruments [46]. A number of LED-IF detection designs for microfluidic chip-based CE [47][48][49][50] systems have been reported as well.…”

Section: ) Australian Centre For Research On Separation Science and mentioning

confidence: 99%

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovičová

Akther

Maaskant

et al. 2015

Analytica Chimica Acta

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Graphical Abstract (please choose)A new generation of integrated miniaturized fibre-coupled solid-state light sources based on microfabricated light emitting diode micro-array (µ-LED), micropackaged with a custom band-pass optical interference filter deposited at the end of an optical fibre, is presented and in this work and demonstrated as excitation light source for fluorescence detection in capillary electrophoresis. ALTERNATIVE IMAGES: Key words:Micro-light emitting diode array; light source; optical fibre; capillary electrophoresis; fluorescence detection; separations. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 *Manuscript (including figures, tables, text graphics and associated captions) Click here to view linked References

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Section: ) Australian Centre For Research On Separation Science and mentioning

confidence: 99%

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovičová

Akther

Maaskant

et al. 2015

Analytica Chimica Acta

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“…For derivatised acids, the solvent must also be compatible with the labelling reagent. We have previously examined a number of different nonaqueous solvents for the separation of a mixture of basic blue dyes, such as NB, by CE [27]. We observed excellent separations of these dyes in ACN, MeOH, EtOH and DMSO in an acetic acid/ammonium acetate electrolyte, but poor separations in other solvents such as DMF, THF and acetone.…”

Section: Nacementioning

confidence: 87%

Separation of Nile Blue‐labelled fatty acids by CE with absorbance detection using a red light‐emitting diode

et al. 2007

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The separation of fatty acids derivatised with Nile Blue (NB) by CE with detection using a red light-emitting diode (LED) was examined. NB was selected as the derivatisation agent due to its high molar absorption coefficient of 76,000 M(-1) cm(-1) at 633 nm, making it well suited for sensitive absorbance detection using a red 635 nm LED. NB-labelled fatty acids were separated by both MEKC using SDS micelles, i-PrOH and n-BuOH and by NACE in a number of solvents including MeOH, EtOH and ACN. The sensitivity of NACE was superior to MEKC, with detection limits of 5x10(-7)-7x10(-7) M obtained for each acid, approximately 20 times lower than the MEKC method. The NACE detection limits are approximately 100 times lower than previous reports on the separation of fatty acids by CE using indirect absorbance detection, ten times lower than using indirect fluorescence detection and are inferior only to those obtained using precapillary derivatisation and direct fluorescence detection. The efficiency of the NACE method was also superior to MEKC and allowed the separation of unsaturated fatty acids to be examined, although it was not possible to baseline-resolve linoleic (C18:2) and linolenic (C18:3) acids in a reasonable time. The method was used to analyse the fatty acid profile of two edible oils, namely sunflower and sesame oils, after alkali hydrolysis, where it was possible to identify both the saturated and unsaturated fatty acids in each sample.

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“…A comprehensive overview of CE applications can be found in the review by Cruces-Blanco et al [5]. In the time frame covered by the present review, Fakhari et al [42] reported a method using nonaqueous CE for the separation of five hydrophobic basic blue dyes (namely: methylene blue, toluidine blue, Brilliant cresyl blue, Nile blue, Victoria blue) followed by detection of absorption of radiation emitted by a red light-emitting diode(LED; maximum at 635 nm). Under optimized conditions, separation occurred in 55 mM ammonium acetate 600 mM acetic acid in methanol.…”

Section: Forensic Drugs Toxicants and Dyesmentioning

confidence: 97%

Recent advances in the applications of CE to forensic sciences (2005–2007)

Tagliaro

Bortolotti

2007

Electrophoresis

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The present article reviews the applications of CE in forensic science covering the period from 2005 until the first part of 2007. The overview includes the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques in the following fields: (i) forensic drugs, toxicants and dyes, (ii) small ions of forensic interest (iii) explosives, (iv) forensic DNA, and (v) other biopolymers of forensic interest.

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Non-aqueous capillary electrophoresis with red light emitting diode absorbance detection for the analysis of basic dyes

Cited by 37 publications

References 20 publications

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Separation of Nile Blue‐labelled fatty acids by CE with absorbance detection using a red light‐emitting diode

Recent advances in the applications of CE to forensic sciences (2005–2007)

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