Non-chemical treatments for preventing the postharvest fungal rotting of citrus caused by Penicillium digitatum (green mold) and Penicillium italicum (blue mold)

Papoutsis, Konstantinos; Mathioudakis, Matthaios M.; Hasperué, Joaquín H.; Ziogas, Vasileios

doi:10.1016/j.tifs.2019.02.053

Cited by 195 publications

(131 citation statements)

References 108 publications

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“…Synthetic fungicides, which have noticeable health or environmental risks, are still the main method used to control them in current fruit storage. Although many nonchemical treatments, mainly including biocontrol agents, natural compounds, UV, hot water treatment, and salts have been used for controlling postharvest fungal diseases on fruit [142,143], these diseases still lead to huge economic losses worldwide every year. Increasing evidence reveals that investigating molecular mechanisms of plant-pathogen interactions is essential for developing novel and safer strategies for durably controlling plant diseases.…”

Section: Discussionmentioning

confidence: 99%

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

et al. 2020

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As the major postharvest disease of citrus fruit, postharvest green mold is caused by the necrotrophic fungus Penicillium digitatum (Pd), which leads to huge economic losses worldwide. Fungicides are still the main method currently used to control postharvest green mold in citrus fruit storage. Investigating molecular mechanisms of plant–pathogen interactions, including pathogenicity and plant resistance, is crucial for developing novel and safer strategies for effectively controlling plant diseases. Despite fruit–pathogen interactions remaining relatively unexplored compared with well-studied leaf–pathogen interactions, progress has occurred in the citrus fruit–Pd interaction in recent years, mainly due to their genome sequencing and establishment or optimization of their genetic transformation systems. Recent advances in Pd pathogenicity on citrus fruit and fruit resistance against Pd infection are summarized in this review.

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Section: Discussionmentioning

confidence: 99%

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

et al. 2020

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“…At this point, development of alternative human/ecofriendly measures to agrochemicals has been an important subject for the scientific world [13]. In this context, numerous studies have been conducted to develop human/ecofriendly alternatives and suggested many biomaterials or measures for the prevention of the postharvest losses: hot water dipping (HWD) [14], hot air treatment (HAT) [15], salts [16], light irradiation [17], modified atmosphere packaging (MAP) [18], edible coatings [19,20], plant extracts [21][22][23][24], essential oils [25,26], chitosan [27,28], and propolis [29,30]. Among these, propolis has an important role in prevention of food loss and waste, thus helping to ensure food safety and security.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Composition of Propolis and Its Efficacy in Maintaining Postharvest Storability of Fresh Fruits and Vegetables

Kahramanoğlu

Okatan

Wan

2020

Journal of Food Quality

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Propolis, also called “bee-glue,” is a natural resinous substance produced by honeybees from plant exudates, beeswax, and bee secretions in order to defend the hives. It has numerous phenolic compounds with more than 250 identified chemical compounds in its composition, which are also known to significantly vary according to the plant sources and season. Moreover, it has a long history in the traditional and scientific medicine as having antibacterial, anticancer, anti-inflammatory, anti-infective, and wound healing effects since 300 BC. In addition to its nutritional and health-promoting effects, it has been reported to improve the postharvest storability of fresh fruits, vegetables, and processed food products. Herein, the biochemical composition and the efficacy of propolis in maintaining the postharvest storability of fresh food products were discussed to provide comprehensive guide to farmers and food processing and storage sectors and to scientists. This review paper also highlights the important points to which special attention should be given in further studies in order to be able to use propolis to develop biopreservatives industrially and for quality preservation during storage.

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“…After harvest, citrus fruits are commercially handled in packing houses in order to maintain their postharvest quality, to increase their shelf life, as well as to reduce losses due to pathogen infection. Citrus blue mold, caused by Penicillium italicum, is one of the most destructive pathogens of postharvest citrus fruits, resulting in losses of up to 80% [2].…”

Section: Introductionmentioning

confidence: 99%

Silencing Dicer-Like Genes Reduces Virulence and sRNA Generation in Penicillium italicum, the Cause of Citrus Blue Mold

Yin

Zhu

Jiang

et al. 2020

Cells

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The Dicer protein is one of the most important components of RNAi machinery because it regulates the production of small RNAs (sRNAs) in eukaryotes. Here, Dicer1-like gene (Pit-DCL1) and Dicer2-like gene (Pit-DCL2) RNAi transformants were generated via pSilent-1 in Penicillium italicum (Pit), which is the causal agent of citrus blue mold. Neither transformant showed a change in mycelial growth or sporulation ability, but the pathogenicity of the Pit-DCL2 RNAi transformant to citrus fruits was severely impaired, compared to that of the Pit-DCL1 RNAi transformant and the wild type. We further developed a citrus wound-mediated RNAi approach with a double-stranded fragment of Pit-DCL2 generated in vitro, which achieved an efficiency in reducing Pi-Dcl2 expression and virulence that was similar to that of protoplast-mediated RNAi in P. italicum, suggesting that this approach is promising in the exogenous application of dsRNA to control pathogens on the surface of citrus fruits. In addition, sRNA sequencing revealed a total of 69.88 million potential sRNAs and 12 novel microRNA-like small RNAs (milRNAs), four of which have been predicated on target innate immunity or biotic stress-related genes in Valencia orange. These data suggest that both the Pit-DCL1 and Pit-DCL2 RNAi transformants severely disrupted the biogenesis of the potential milRNAs, which was further confirmed for some milRNAs by qRT-PCR or Northern blot analysis. These data suggest the sRNAs in P. italicum that may be involved in a molecular virulence mechanism termed cross-kingdom RNAi (ck-RNAi) by trafficking sRNA from P. italicum to citrus fruits.

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Non-chemical treatments for preventing the postharvest fungal rotting of citrus caused by Penicillium digitatum (green mold) and Penicillium italicum (blue mold)

Cited by 195 publications

References 108 publications

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

Biochemical Composition of Propolis and Its Efficacy in Maintaining Postharvest Storability of Fresh Fruits and Vegetables

Silencing Dicer-Like Genes Reduces Virulence and sRNA Generation in Penicillium italicum, the Cause of Citrus Blue Mold

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