Non-contact ultrasound oocyte denudation

Mokhtare, Amir; Davaji, Benyamin; Xie, Philip; Yaghoobi, Mohammad Ali; Rosenwaks, Zev; Lal, Amit; Palermo, Gianpiero D.; Abbaspourrad, Alireza

doi:10.1039/d1lc00715g

Cited by 7 publications

(4 citation statements)

References 87 publications

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“…Recently, Mokhtare et al published an ultrasound oocyte denudation chip. 33 Although the introduced chip had a high speed in denudation, the safety of the oocyte in the face of the direct radiation of ultrasound waves from a highly close distance is still unknown, and this concern is a serious obstacle to its use in laboratories. Other works attempted oocyte cumulus cell denudation by employing suction force to remove cumulus cells around the oocyte.…”

Section: Resultsmentioning

confidence: 99%

Design and implementation of a lab-on-a-chip for assisted reproductive technologies

Safaefar,

Karamdel,

Veladi

et al. 2023

Bioimpacts

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Introduction: The microfluidic device is highly optimized to remove oocytes from the cumulus-corona cell mass surrounding them. Additionally, it effectively captures and immobilizes the oocytes, aiding in assessing their quality and facilitating the injection of sperm into the oocyte. In this study, a novel microfluidic chip was designed and manufactured using conventional soft lithography methods. Methods: This research proposes the utilization of a microfluidic chip as a substitute for the conventional manual procedures involved in oocyte denudation, trapping, and immobilization. The microfluidic chip was modeled and simulated using COMSOL Multiphysics® 5.2 software to optimize and enhance its design and performance. The microfluidic chip was fabricated using conventional injection molding techniques on a polydimethylsiloxane substrate by employing soft lithography methods. Results: A hydrostatic force was applied to guide the oocyte through predetermined pathways to eliminate the cumulus cells surrounding the oocyte. The oocyte was subsequently confined within the designated trap region by utilizing hydraulic resistance along the paths and immobilized by applying vacuum force. Conclusion: The application of this chip necessitates a lower level of operator expertise compared to enzymatic and mechanical techniques. Moreover, it is feasible to continuously monitor the oocyte's state throughout the procedure. There is a reduced need for cultural media compared to more standard approaches.

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Section: Resultsmentioning

confidence: 99%

Design and implementation of a lab-on-a-chip for assisted reproductive technologies

Safaefar,

Karamdel,

Veladi

et al. 2023

Bioimpacts

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“…It also can rely on oocyte denudation: where oocytes are denuded by mechanical and enzymatic treatment to be prepared for the injection during ICSI; and due to this, the nuclear maturity of the oocytes can be evaluated to deduce the most suitable ones [25]. Microfluidic chips could help automate and shorten the time required for denudation procedures, while also improving quality of oocytes due to lower shear stress and damage on them during the procedure [26] [27].…”

Section: Gamete Analysismentioning

confidence: 99%

Is the world ready for human germline genetic editing using CRISPR/Cas9?

Manne

2023

Preprint

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The use of gene editing (GE) technologies like CRISPR/Cas9 on human embryos constitutes a controversial application that would primarily rely on in vitro fertilization (IVF) and preimplantation genetic testing (PGT) technologies to be applicable clinically. In today's world, IVF is rapidly advancing through artificial intelligence, time-lapse microscopic cinematography, and microfluidics, thereby edging closer to this ethically complex application. PGT results, on the other hand, could possibly be unrepresentative of embryo genomes due to the normalized testing of extraembryonic cell lineages (that form the placenta), instead of testing embryonic cell lineages (that form the embryo): a critical error that is based on outdated science. While the use of CRISPR/Cas9 on human embryos has varied results in different studies, some approaches to the gene-editing tool could prove more effective on embryos than others. The discussion of to what extent this tool should be used has received significant attention in the scientific community. Although, a lack of global consensus gives no clear answer to this essential question for research to continue in this field. This paper will discuss IVF and PGT in the context of human germline gene editing using CRISPR/Cas9, as well as the gene-editing tool's applications, limitations, and ethical considerations on human embryos.

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“…15 Microfluidic devices have made it easier to study and select for sperm features by implementing gentle flows, 16,17 filter-like components, 18,19 and the possibility of automation. 20 These devices also include investigations of the four navigational mechanisms of sperm cells; thigmotaxis (swimming along boundaries), thermotaxis (swimming against the direction of a temperature gradient), chemotaxis (swimming against chemical gradients) and rheotaxis (swimming against the flow). 4 Chemotaxis and thermotaxis are short range mechanisms that guide sperm toward the egg in the oviduct and are only active in approximately 10% of the sperm population in mammalian species.…”

Section: Introductionmentioning

confidence: 99%

Faster sperm selected by rheotaxis leads to superior early embryonic development in vitro

Yaghoobi,

Abdelhady,

Favakeh

et al. 2024

Lab Chip

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Non-contact ultrasound oocyte denudation

Abstract: Cumulus removal (CR) is a central prerequisite step for many protocols involved in the assisted reproductive technology (ART) such as intracytoplasmic sperm injection (ICSI) and preimplantation genetic testing (PGT). The...

Cited by 7 publications

References 87 publications

Design and implementation of a lab-on-a-chip for assisted reproductive technologies

Design and implementation of a lab-on-a-chip for assisted reproductive technologies

Is the world ready for human germline genetic editing using CRISPR/Cas9?

Faster sperm selected by rheotaxis leads to superior early embryonic development in vitro

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