Non-destructive identification of physical damage in mung bean (Vigna radiata L.) seeds by X-ray image analysis

Abstract

Global demand for pulses such as the mung bean has grown in the last years. For successful production of these crops it is necessary to use high quality seeds. Methodologies based on X-ray image analysis have been used as a complementary tool to evaluate the physical quality of seeds due to their speed and potential for automation. The aim of this study was to evaluate the efficiency of X-ray analysis for non-destructive evaluation of the physical quality of Vigna radiata seeds and to relate the variables obtained with their physiological potential. For this, seeds from eight lots were X-rayed and subsequently subject to germination test. In total, 18 physical and physiological parameters were determined. The X-ray image analysis was efficient for evaluating the internal morphology of Vigna radiata seeds and allowed the identification of various damage types. However, it was not possible to relate the physical variables to the seed quality as the lots presented similar germination percentage. Physical variables such as solidity and circularity are related to percentage of root protrusion and length of seedling hypocotyl. Low relative densities indicate deteriorated tissues, related to severe morphological damage and non-viable seeds.

X-ray, multispectral and chlorophyll fluorescence images: innovative methods for evaluating the physiological potential of rice seeds

“…In rice seeds, with the lots under study, this parameter showed no correlation with the physiological potential (Figure 5B). Similar results were also observed in mung bean (Vigna radiata L.) seeds by Machado et al (2020), who found that the relative density depends on characteristics of the seed of the studied species, such as oil and carbohydrate contents, thickness, among others.…”

FRUIT AND SEED MORPHOLOGY, AND GERMINATION OF Quesnelia quesneliana (BRONGNIART) L.B. SMITH

“…The germinative behavior of Q. quesneliana can be complemented by the t50, uniformity of germination, mean germination time, and germination speed coefficient (Figure 3). Recently, these and other similar indexes have been often used to assess the vigor of agricultural species such as Brachiaria grass (MEDEIROS et al, 2020a), melon (MEDEIROS et al, 2020b), mung bean (MACHADO et al, 2020), and others. However, although there was no comparison between different lots, they are unexplored in Bromeliads and may bring future contributions to research with these species.…”

Época de colheita e beneficiamento na qualidade de sementes de Crambe abyssinica durante o armazenamento

“…O estudo da morfometria das sementes pode trazer benefícios para programas de melhoramento genético (TANABATA et al, 2012). Informações desse tipo demonstram alta relação com o vigor das plântulas JAVORSKI et al, 2018;MEDEIROS et al, 2020).…”

“…Em sementes de melão (Cucumis melo L.), a análise de raios-X permitiu a observação das estruturas internas e a diferenciação de cultivares. Nessa espécie, a densidade relativa e densidade integrada, que são variáveis relacionadas ao número de pixels na imagem, se destacaram em predizer sobre o potencial fisiológico das sementes, o que demonstra o nicho de variáveis que pode ser retirado dessa análise (MEDEIROS et al, 2020).…”

“…Nos últimos anos cresceram as pesquisas com uso de métodos baseados em imagens radiográficas, demonstrando a eficiência da técnica na avaliação da qualidade de sementes. Algumas espécies estudas são: brócolis -Brassica oleracea; abóbora -Cucurbita moschata(ANTONIO et al, 2016); jiló -Solanum gilo(PRADO-ALVES et al, 2018); feijão mungu verde -Vigna radiata(MACHADO et al, 2020); pimenta Habanero -Capsicum chinenses(MEDEIROS et al, 2020a) e melão -Cucumis melo(MEDEIROS et al, 2020b).…”