Non-enzymatic antioxidant status and modulation of lipid peroxidation in the muscles of Labeo rohita by sub lethal exposure of CuSO4

Jena, Srikanta; Behera, Madhumita; Dandapat, Jagneshwar; Mohanty, Nakulananda

doi:10.1007/s11259-008-9188-x

Cited by 17 publications

(8 citation statements)

References 29 publications

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“…As evidence, inhibition of liver GSH was seen in three spined sticklebacks (Gasterosteus aculeatus) upon exposure to copper sulfate (Sanchez et al, 2005). In support of this study, Jena et al, 2009 showed the depletion of muscle GSH after copper sulphate exposure. The significant reduction in the GSH level is mainly due to direct copper interference with GSH synthesis by inhibition of glutathione reductase.…”

Section: Glutathione (Gsh)supporting

confidence: 77%

A critical review on integrating multiple fish biomarkers as indicator of heavy metals contamination in aquatic ecosystem

Hemmadi¹

2017

IJB

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An immense amount of data is available on biomarkers related to different eco-toxicants. But data on contaminant-specific biomarkers in fishes is sparse. Traditionally, detection and quantification of heavy metals in sediment, water, and biota gave us valuable information on the quantity and the type of heavy metal present in the ecosystem. This information can be utilized to select a heavy metal specific biomarker. For an instance, if Cadmium (Cd), Zinc (Zn) and Cupper (Cu) are at high concentration, then Metallothionein (MT) can be a good candidate biomarker. Along with this, Superoxide dismutase (SOD) is a very potent indicator of Iron (Fe) and Mercury (Hg) contamination and also Catalase (CAT) is specific for Cadmium (Cd) and Zinc (Zn) exposure. For these kinds of selection of biomarker, the researchers should know heavy metals type specific biomarker. This review is the small effort towards cumulating the heavy metals type specific biomarker. This demonstrates the exposure and effects of heavy metals in fishes by integrating the heavy metal quantification and biomarker selection.

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Section: Glutathione (Gsh)supporting

confidence: 77%

A critical review on integrating multiple fish biomarkers as indicator of heavy metals contamination in aquatic ecosystem

Hemmadi¹

2017

IJB

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“…Gravato et al (2006) attribute the depletion of GSH to direct copper interference with GSH synthesis, inhibition of glutathione reductase, and the participation of GSH as a substrate in detoxification reactions. The depletion of GSH in fish muscle after copper sulphate exposure has also been reported (Jena et al, 2009).…”

Section: Coppermentioning

confidence: 81%

Metals as a cause of oxidative stress in fish: a review

Ševčíková¹,

Modrá²,

Slaninova³

et al. 2011

Vet. Med.

319

123

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ABSTRACT:This review summarizes the current knowledge on the contribution of metals to the development of oxidative stress in fish. Metals are important inducers of oxidative stress in aquatic organisms, promoting formation of reactive oxygen species through two mechanisms. Redox active metals generate reactive oxygen species through redox cycling, while metals without redox potential impair antioxidant defences, especially that of thiol-containing antioxidants and enzymes. Elevated levels of reactive oxygen species lead to oxidative damage including lipid peroxidation, protein and DNA oxidation, and enzyme inactivation. Antioxidant defences include the enzyme system and low molecular weight antioxidants. Metal-binding proteins, such as ferritin, ceruloplasmin and metallothioneins, have special functions in the detoxification of toxic metals and also play a role in the metabolism and homeostasis of essential metals. Recent studies of metallothioneins as biomarkers indicate that quantitative analysis of mRNA expression of metallothionein genes can be appropriate in cases with elevated levels of metals and no evidence of oxidative damage in fish tissue. Components of the antioxidant defence are used as biochemical markers of oxidative stress. These markers may be manifested differently in the field than in results found in laboratory studies. A complex approach should be taken in field studies of metal contamination of the aquatic environment. Keywords: ROS; metallothioneins; glutathion; superoxide dismutase; antioxidant defenceList of abbreviations ALA-D = aminolevulinic acid dehydratase; BNF = β-naphthoflavone; CAT = catalase; GPx = glutathione peroxidise; GR = glutathione reductase; GSH = glutathione; GSSG = glutathione disulphide; GST = glutathione S-transferase; LPO = lipid peroxidation; MDA = malondialdehyde; MTs = metallothioneins; NADPH = nicotinamidadeninedinucleotide phosphate (reduced); ROS = reactive oxygen species; SOD = superoxide dismutase

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“…An increased ASC pool and ASC/DHA value is an indicator of the adaptive response to limit the oxidative damage in fish [46], [85]. The high levels of reduced [ASC] in response to HEA connote an advantage for the carp and goldfish to resist oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Anti-Oxidative Defences Are Modulated Differentially in Three Freshwater Teleosts in Response to Ammonia-Induced Oxidative Stress

et al. 2014

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Oxidative stress and the antioxidant response induced by high environmental ammonia (HEA) were investigated in the liver and gills of three freshwater teleosts differing in their sensitivities to ammonia. The highly ammonia-sensitive salmonid Oncorhynchus mykiss (rainbow trout), the less ammonia sensitive cyprinid Cyprinus carpio (common carp) and the highly ammonia-resistant cyprinid Carassius auratus (goldfish) were exposed to 1 mM ammonia (as NH4HCO3) for 0 h (control), 3 h, 12 h, 24 h, 48 h, 84 h and 180 h. Results show that HEA exposure increased ammonia accumulation significantly in the liver of all the three fish species from 24 h–48 h onwards which was associated with an increment in oxidative stress, evidenced by elevation of xanthine oxidase activity and levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Unlike in trout, H2O2 and MDA accumulation in carp and goldfish liver was restored to control levels (84 h–180 h); which was accompanied by a concomitant increase in superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase activity and reduced ascorbate content. Many of these defence parameters remained unaffected in trout liver, while components of the glutathione redox cycle (reduced glutathione, glutathione peroxidase and glutathione reductase) enhanced to a greater extent. The present findings suggest that trout rely mainly on glutathione dependent defensive mechanism while carp utilize SOD, CAT and ascorbate as anti-oxidative sentinels. Hepatic cells of goldfish appear to utilize each of these protective systems, and showed more effective anti-oxidative compensatory responses towards HEA than carp, while trout were least effective. The present work also indicates that HEA exposure resulted in a relatively mild oxidative stress in the gills of all three species. This probably explains the almost complete lack of anti-oxidative responses in branchial tissue. This research suggests that oxidative stress, as well as the antioxidant potential clearly differ between salmonid and cyprinid species.

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Non-enzymatic antioxidant status and modulation of lipid peroxidation in the muscles of Labeo rohita by sub lethal exposure of CuSO4

Cited by 17 publications

References 29 publications

A critical review on integrating multiple fish biomarkers as indicator of heavy metals contamination in aquatic ecosystem

A critical review on integrating multiple fish biomarkers as indicator of heavy metals contamination in aquatic ecosystem

Metals as a cause of oxidative stress in fish: a review

Anti-Oxidative Defences Are Modulated Differentially in Three Freshwater Teleosts in Response to Ammonia-Induced Oxidative Stress

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