Non-enzymatic electrochemical approaches to cholesterol determination

Derina, K. V.; Короткова, Е. И.; Barek, Jiřı́

doi:10.1016/j.jpba.2020.113538

Cited by 27 publications

(13 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, detecting the Cho sensitively and accurately is critical for disease control and prevention. Several methods for determining Cho have been published, including high performance liquid chromatography (HPLC), colorimetry, fluorimetry, and electrochemistry methods. − Among them, the electrochemical method is the most popular due to its rapidity, simplicity, low cost, satisfactory reproducibility, and user-friendliness. − …”

Section: Introductionmentioning

confidence: 99%

Radix Pueraria Flavonoids Assisted Green Synthesis of Reduced Gold Nanoparticles: Application for Electrochemical Nonenzymatic Detection of Cholesterol in Food Samples

et al. 2022

View full text Add to dashboard Cite

Using radix pueraria flavonoids (RPFs) as a reducing and stabilizing agent, we report a simple, cost-effective, and ecologically friendly green synthesis technique for gold nanoparticles (AuNPs) in the present study. Ultraviolet−visible (UV) spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) investigations were used to characterize the AuNPs. The results demonstrated that the produced AuNPs were nearly spherical and that their particle sizes had a mean diameter of 4.85 ± 0.75 nm. The "Green" AuNPs, exhibiting remarkable peroxidase-like activity and Michaelis−Menten kinetics with high affinity for H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB), were effectively applied to the fabrication of a sensitive nonenzymatic enhanced electrochemical sensor for the detection of cholesterol (Cho). Under optimum circumstances, it was possible to establish two linear ranges of 1−100 and 250−5000 μmol/L with a detection limit of 0.259 μmol/L (signal/noise ratio (S/N) = 3). The suggested sensor was utilized with satisfactory findings to determine the amount of Cho in food samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Radix Pueraria Flavonoids Assisted Green Synthesis of Reduced Gold Nanoparticles: Application for Electrochemical Nonenzymatic Detection of Cholesterol in Food Samples

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Even though they perform satisfying for cholesterol detection, most of them are burdensome, time-consuming, require sample pre-treatment, a high-cost instrumental set-up, difficult standardization, and experienced personnel to operate. An electrochemical sensing approach overcomes these disadvantages and the worth of cholesterol (bio)sensors is already recognized and visible from the vast research in this field summarized in comprehensive reviews [ 11 , 12 , 13 , 14 ]. Most of the electrochemical assays for cholesterol are enzyme-based, where cholesterol oxidase (ChOx) is employed as the sensing elements and different electron mediators provide an appropriate potential gradient for electron transfer between the enzyme’s active site and electrode [ 13 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Innovative Non-Enzymatic Electrochemical Quantification of Cholesterol

Carp¹,

Pinteala²,

Arvinte³

2022

Sensors

View full text Add to dashboard Cite

The use of the Liebermann–Burchard reaction in this study has been explored in the development of a simple, reliable, and robust quantitative electrochemical method to assay cholesterol, and hence provide a good alternative to colorimetric methods. The optimization of batch mode operation for electrochemical oxidation of cholesterol in the Liebermann–Burchard reagents included the applied potential and acidic volume. Tested using chronoamperometry, the developed method showed a high sensitivity (14.959 μA mM−1) and low detection limit (19.78 nM) over a 0.025–3 mM concentration range, with remarkable linearity (R2  =  0.999), proving an analytical performance either higher or comparable to most of the cholesterol sensors discussed in literature. The influence of possible interfering bioactive agents, namely, glucose, uric acid, ascorbic acid, KCl and NaCl, has been evaluated with no or negligible effects on the measurement of cholesterol. Our study was directed at finding a new approach to chemical processing arising from the use of external potential as an additional level of control for chemical reactions and the transfer of electrons between surfaces and molecules. Finally, the optimized method was successfully applied for the determination of cholesterol content in real blood samples.

show abstract

“…The American Heart Association suggests the amount of cholesterol for adults between 40 and 75 years of age to be between 1.81-4.90 mM (0.7-1.89 mg mL -1 ). 5 Too low cholesterol levels (hypocholesterolemia), on the other hand, result in anaemia and hepatopathy. 2 Determination of the exact level of cholesterol in serum remains a major diagnostic challenge.…”

Section: Introductionmentioning

confidence: 99%

“…Non-enzymatic sensors with the same detection limits and cholesterol specificity are valuable alternatives, but are still not widely developed. 5,12,13 While cholesterol undergoes direct electrochemical oxidation on platinum electrodes, 14,15 this approach seems not well-adapted for direct cholesterol sensing. The use of a displacement indicator assay (IDA), by exploiting surfaceconfined host-guest interactions, represents an elegant approach for enzyme-free electrochemical cholesterol sensing.…”

Section: Introductionmentioning

confidence: 99%

Non-enzymatic electrochemical cholesterol sensor based on strong host-guest interactions with a polymer of intrinsic microporosity (PIM) with DFT study

et al. 2021

View full text Add to dashboard Cite

Advances in materials science has accelerated the development of diagnostic tools with the last decade witnessing the development of enzyme-free sensors, owing to the improved stability, low cost, and simple fabrication of component materials. However, the specificity of non-enzymatic sensors for certain analytes still represents a challenging task, for example, the determination of cholesterol level in blood is vital due to its medical relevance. In this work, a reagent displacement assay for cholesterol sensing in serum samples was developed. It is based on coating of a glassy carbon electrode with a polymer of intrinsic micro-porosity (PIM) that forms a host-guest complex with methylene blue (MB). In the presence of cholesterol, the MB electroactive probe was displaced due to the stronger association of cholesterol guest to the PIM host. The decrease in the oxidative current was proportional to the cholesterol concentration achieving a detection limit of approximately 0.1 nM.

show abstract

Non-enzymatic electrochemical approaches to cholesterol determination

Cited by 27 publications

References 76 publications

Radix Pueraria Flavonoids Assisted Green Synthesis of Reduced Gold Nanoparticles: Application for Electrochemical Nonenzymatic Detection of Cholesterol in Food Samples

Radix Pueraria Flavonoids Assisted Green Synthesis of Reduced Gold Nanoparticles: Application for Electrochemical Nonenzymatic Detection of Cholesterol in Food Samples

Innovative Non-Enzymatic Electrochemical Quantification of Cholesterol

Non-enzymatic electrochemical cholesterol sensor based on strong host-guest interactions with a polymer of intrinsic microporosity (PIM) with DFT study

Contact Info

Product

Resources

About